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Preparation for repairing petroleum hydrocarbon pollutants and application of preparation

A technology of petroleum hydrocarbons and pollutants, applied in the field of soil remediation, to achieve the effects of improving efficiency, saving resources, and reducing production costs

Pending Publication Date: 2019-12-20
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the existing problems in the oil-contaminated soil remediation technology of chemical leaching and microbial agents, and provides a preparation for remediating petroleum hydrocarbon pollutants and its application to improve the efficiency of microbial agents in remediating contaminated soil

Method used

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  • Preparation for repairing petroleum hydrocarbon pollutants and application of preparation
  • Preparation for repairing petroleum hydrocarbon pollutants and application of preparation
  • Preparation for repairing petroleum hydrocarbon pollutants and application of preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of Immobilized Microbial Materials

[0036] (1) Biochar is prepared from agricultural waste biomass corn stalks as raw material. Remove impurities from the straw, dry it naturally for 7 days after cleaning, and preliminarily pulverize it to a size of 1-2 cm, dry it at 50 °C for 4 hours, put the straw into a muffle furnace after drying, and pass high-purity nitrogen for 20 minutes before heating to form an oxygen-free environment, the heating rate of 5 °C / min was heated to 300, 400, and 500 °C, respectively, and pyrolyzed at each temperature for 2 hours to obtain biochar materials with different pyrolysis temperatures; Save for backup.

[0037] (2) Resuscitate and culture Acinetobacter HC8-3S and Dijianella CN-3 stored in -80°C refrigerator, respectively, transfer the resuscitated bacteria liquid to 2216E medium, at 30°C, 150rpm Expand the culture in a constant temperature shaker to obtain bacterial suspensions of the two strains of bacteria, and mix the tw...

Embodiment 2

[0043] Remediate contaminated soil, such as figure 1 shown:

[0044] 1) Surfactant to clean contaminated soil

[0045] Pulverize the oil-contaminated soil, remove impurities, dry it outdoors for 8 hours, store it in a square shallow dish, add a cleaning solution, (the cleaning solution is a cleaning solution, a single-surfactant cleaning solution or a mixed-surfactant cleaning solution), and place the shallow dish in the Stir at room temperature for 24h under agitator, let it settle naturally after mixing, discard the liquid phase, take out the solid phase and air-dry in a dark and ventilated place, seal the shallow dish for later use, and measure the content of petroleum hydrocarbons in the soil (see figure 2 ).

[0046] Among them, the final concentration of surfactant in the washing solution is 3g / L, the final concentration of auxiliary agent is 5g / L, the mass ratio of surfactant is SDS:TW-80:TX-100=2:2:1, and the mass of auxiliary agent is The ratio of sodium lignosulf...

Embodiment 3

[0052] The bacterial liquid obtained in the step (2) of the above embodiment 1, the biochar obtained in the step (1), and the immobilized microbial material obtained above at a pyrolysis temperature of 500 ° C are respectively mixed with the contaminated soil, degraded, and the content of petroleum hydrocarbons in the soil is determined, ( see Figure 4 )

[0053] At the same time, the above-mentioned immobilized microbial material obtained at a pyrolysis temperature of 500 ° C was added to the oil-contaminated soil after washing with the cleaning solution, and then water was added to make the soil moisture content reach 25%, an appropriate amount of normal soil and wood chips were added, and the mixture was fully stirred. The shallow dish is placed under natural conditions, the temperature is 20-26 ℃, and the water is regularly replenished, turned evenly, and NH4Cl and K2HPO4 are added to make the N and P concentrations in the soil reach 2000 mg / kg and 200 mg / kg respectively;...

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Abstract

The invention belongs to the technical field of soil remediation, and particularly relates to a preparation for repairing petroleum hydrocarbon pollutants and an application of the preparation. The preparation is prepared through fixing microorganisms in charcoal materials obtained through splitting, wherein the single carrying capacity of the microorganisms in the coal materials is 3.2-6.5*10<10>cfu / g, and the microorganisms are acinetobacter sp. HC8-3S and Dietzia sp.N3ZF-1 CN-3. Surfactant cleaning liquid compounded from the preparation is high in cleaning efficiency and small in soil remaining. The prepared charcoal immobilized microorganism material has the advantages that the fixing rate is high, the production cost is low, soil microecological environment improvement is promoted, and secondary pollution is not caused to environment, and the preparation is used for repairing soil polluted by petroleum and petroleum products.

Description

technical field [0001] The invention belongs to the technical field of soil remediation, in particular to a preparation for remediating petroleum hydrocarbon pollutants and its application. Background technique [0002] Oil is one of the important energy sources in modern industry, and various leakage pollution incidents occur frequently in the process of oil exploration, transportation, storage and processing, destroying the soil ecosystem, enriching through the food chain, endangering human health, and the problem of soil oil pollution is becoming more and more prominent. . [0003] At present, it can be disposed of by landfill, incineration, extraction and other methods, but these methods all have problems such as high price and secondary pollution. Microbial remediation technology is the use of indigenous microorganisms to absorb, transform, remove or degrade organic pollutants, and is an important method for bioremediation to deal with oil pollution. At present, there...

Claims

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Application Information

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IPC IPC(8): C12N11/14B09C1/02B09C1/10C12R1/01
CPCC12N11/14B09C1/02B09C1/10B09C2101/00
Inventor 彭健胡晓珂孙延瑜宋虹
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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