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Use of aquaporin 4 as a drug target for depression

An aquaporin and depression technology, applied in the field of biomedicine, can solve the problems of unclear pathogenesis of depression, hypotheses of abnormal function and neurotrophic factors, limited treatment drugs, etc.

Active Publication Date: 2021-08-31
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the pathogenesis of depression has not been clearly analyzed. There are multiple biological hypotheses for the occurrence of depression, including monoaminergic neurotransmitter hypothesis, brain reward pathway damage hypothesis, hypothalamus-pituitary-adrenaline axis (HPA axis) Hypothesis of abnormal function and neurotrophic factor hypothesis, etc., resulting in very limited alternative drug targets and effective drugs for the treatment of depression

Method used

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  • Use of aquaporin 4 as a drug target for depression
  • Use of aquaporin 4 as a drug target for depression
  • Use of aquaporin 4 as a drug target for depression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Establishing a depression model

[0035] 120 SPF grade KM mice were adaptively fed for 1 week and randomly divided into normal group and model group. Two weeks after modeling, the model group was randomly divided into a depression model group, a stilbene glycoside group, an emodin group, an emodin glucoside group, an emodin group, and an injection-made Shouwu powder administration group according to body weight.

[0036] Chronic mild unpredictable stimuli include 10 kinds: tail pinch 5 minutes / time, horizontal shock 20 minutes / time, deprivation of water (12 hours), swimming in ice water (4°C), fasting for 12 hours, mouse cage (tilted Angle of 45 degrees) inclined, wet bedding (not too wet), fasting and water restriction, placement of foreign objects (paper scraps, orange peels, etc.), odor stimulation.

[0037] Simulate the chronic low-intensity stress received by humans in daily life, and ensure that one different stimulus is arranged every day, the order i...

Embodiment 2

[0042] Embodiment 2: Injection administration and sample collection

[0043] 1. Administration by injection

[0044] After the mouse model was established, the drug injection was started, wherein the injection drug powder group was formulated with 5g / kg powder, and the stilbene glycoside group, rhubarb group, emodin glucoside group, and emodin group were formulated with 100 mg / kg drug . Continuous injection for 15 days.

[0045] 2. Eyeball blood collection, hippocampus tissue extraction

[0046] All mice were subjected to the eyeball blood test, 1 mL of blood was collected, centrifuged for 20 min, and the speed was 3000 rpm. Take the supernatant and store in the refrigerator. Take out the brain tissue, cut off the cerebellum, separate the brain along the midline, put the cerebral cortex down and the inner structure up on the ice pack, and carefully peel off the white matter with curved forceps. The hippocampus is crescent-shaped inside near the side of the cerebellum. Sto...

Embodiment 3

[0047] Example 3: Detection of the expression level of aquaporin 4

[0048] 1. The expression level of AQP4 in serum

[0049] 1. ELISA

[0050] (1) Experimental method, comprising the following steps:

[0051] S1: Let the antigen bind to the surface of the solid phase carrier first, and ensure the immunological activity of the antigen;

[0052] S2: Let the antigen or antibody be linked with an enzyme that can mark the antigen antibody and maintain the activity of the enzyme to form an enzyme-labeled antigen or antibody;

[0053] S3: When starting the experimental detection, react the labeled specimen with the enzyme-labeled antigen and the antigen on the solid phase carrier in the first step.

[0054] S4: Washing to separate the complexes formed by the reaction from other impurities. Then there is a certain proportional relationship between the amount of enzyme reaction in the sample and the amount of enzyme bound on the solid phase. Because after adding the enzyme-reacti...

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Abstract

The invention discloses the application of aquaporin 4 as a drug target for preventing and treating depression. The research results of the present invention show that AQP4 has a direct correlation with the onset and treatment of depression. Depression can block the expression of AQP4 in serum and reduce its content. , the content of AQP4 increased; after suffering from depression, the expression of AQP4 in the hippocampus was relatively reduced, and after treatment with drugs such as stilbene glycoside and emodin, the expression of AQP4 increased. AQP4 is a new therapeutic target for depression.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the use of aquaporin 4 as a drug target for depression. Background technique [0002] Depression is a common psychiatric disease. According to the statistics of the World Health Organization, depression has become the fourth largest disease in the world and the second largest disease burden in China. It is predicted that by 2020, it may become the second largest disease after coronary heart disease. Diseases account for 15% of the global burden of disease, and the lifetime incidence of depression is between 6% and 8%. With the gradual aging of the population, the incidence of depression among people over 60 years old will be as high as 20% to 50% %. Depressed patients are high-risk groups of suicide, and there is always the risk of suicide from the onset of the disease to the recovery period. Depression is under-diagnosed and effectively cured. At present, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68A61K49/00
CPCA61K49/0008G01N33/68G01N2500/00G01N2800/28
Inventor 吴晓青李金圣蒋合众毛建飞
Owner SOUTHWEST JIAOTONG UNIV
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