Rapid PCR amplification kit as well as application method thereof

Abstract

The invention provides a rapid PCR amplification kit as well as an application method thereof. The kit comprises DNA polymerase, dNTPs, internal reference gene primers and buffer fluid lines, whereinthe internal reference gene primers comprise an internal parameter A fragment and an internal parameter B fragment, the internal parameter A fragment comprises an upstream primer F and a downstream primer R, and the internal parameter B fragment comprises an upstream primer F and a downstream primer R. According to the rapid PCR amplification kit as well as the application method thereof, combinedwith Phire heat to start IIDNA polymerase, amplification of longer fragment targets can be realized, mistake rate is lower, a PCR enhancer is added into the kit, a high-level structure of a nucleotide can be opened, the kit has the characteristics by being convenient, saving money and improving efficiency, and rapid amplification of trace DNA samples is realized through one-step PCR detection.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a rapid PCR amplification kit and an application method thereof. Background technique [0002] PCR is the abbreviation of polymerase chain reaction. It is a molecular biology technique used to amplify specific DNA fragments. Its research has a profound impact on various fields of biology, and its relationship with medicine, agriculture, bioengineering, etc. Very closely, for example, PCR technology involves new ways of breeding in agriculture, medical detection and treatment of genetic diseases, emerging industries based on genetic engineering, forensic evidence identification, paternity testing, etc. Rapid PCR includes two parts: rapid nucleic acid extraction and PCR amplification. The classic PCR amplification kit includes PCR buffer system, dNTPs, DNA polymerase, etc. In order to prepare the PCR reaction solution in the existing kits, different reagents are repeat...

AI Technical Summary

Problems solved by technology

However, due to the little DNA residue at the crime scene and the traces of forensic evidence DNA collected are extremely small, the reaction sensitivity of conventional PCR amplification methods is low, it is difficult to capture effective DNA information, and the amplification effect is poor, which directly leads to subsequent DNA testing. The detection rate is low, STR typing fails, and criminal individuals cannot be identified

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