Probiotic fermented food for regulating intestinal health and preparation method thereof
A technology for regulating intestinal tract and probiotics, applied to bacteria, lactobacilli, and small cocci used in food preparation, can solve problems such as inability to guarantee adaptability and product effect, large loss of active ingredients, and easy contamination of products. Achieve the effect of rich metabolite content, maintain intestinal health, and excellent health care effect
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Embodiment 1
[0048] Example 1: Preparation method of probiotic fermented functional food that regulates intestinal health
[0049] 1. Determination of the inoculation amount of probiotics
[0050] (1) Wash the soybeans and dry them in an oven at 60°C for 5-6 hours, and grind them through a 100-mesh sieve to obtain soybean powder;
[0051] (2) Preparation of activated medium: add 12 g of soybean powder per 100 ml of water to obtain activated medium; sterilize the activated medium under the conditions of 121 ° C for 20 minutes;
[0052] (3) Configure fermentation medium: get soybean flour: water=1: 1.4 (mass volume ratio), mix and place in a 6T solid-state fermentation tank for sterilization to obtain a solid-state fermentation medium for subsequent use; 6T solid-state fermentation tank sterilization conditions For, 121℃, 20min;
[0053] (4) Strain activation: Lactobacillus reuteri, Bifidobacterium longum, Pediococcus lactis, Lactobacillus plantarum, Bifidobacterium animalis, Lactobacillus...
Embodiment 2
[0098] Embodiment 2: Experimental research on improving intestinal flora structure Experimental method:
[0099] 1. Preparation of probiotic fermented food for regulating intestinal health: after preparing probiotic fermented food according to Example 1-4, freeze-dry in a vacuum freeze dryer for 30 hours.
[0100] 2. Experimental animals: 90 male Kunming mice weighing 18-22 g and weaned for one month were purchased from the Experimental Animal Center of the Fourth Military Medical University of the Chinese People's Liberation Army.
[0101] 3. Experimental reagents: Bifidobacterium selective medium (BBL), Lactobacillus selective medium (Lbs), eosin methylene blue agar, sodium azide-crystal violet-escin agar, tryptone-sulfite - Cycloserine agar base (TSC), D-cycloserine solution, physiological saline.
[0102] 4. Experimental procedure: 90 mice were equally divided into blank group, experimental group 1, and experimental group 2. The feces of mice in each group were asepticall...
Embodiment 3
[0110] Embodiment 3: clinical treatment effect
[0111] The probiotic fermented food obtained in Example 1-4 is used for clinical trials after being freeze-dried in a vacuum freeze dryer for 30h, 20 patients in each case, half and half men and women, orally for seven days, twice a day, each time 5g, the therapeutic effect is shown in Table 6.
[0112] Table 6 The therapeutic effect of this patented product on different intestinal diseases
[0113] disease cure markedly effective efficient invalid diarrhea 20 0 0 0 constipate 20 0 0 0 colitis 18 2 0 0
[0114] Judging from the results of clinical experiments, the probiotic fermented food invented by this patent has excellent therapeutic effects on various intestinal diseases, and has a two-way regulating effect on diarrhea and constipation.
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