High-quality bran raw material and preparation method and application thereof
A high-quality, bran-based technology, applied in the field of bioengineering, can solve the problems of fermentation to increase the processing applicability of active substances, special flavor substances, etc., and achieve the effects of increasing water solubility index, increasing protein content, and improving swelling degree
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Embodiment 1
[0031] 1. Wash and filter out the mixed flour in the wheat bran purchased in the market, dry it, beat it into powder, pass it through a 40-mesh sieve, and sterilize it in an autoclave at 121°C for 20 minutes, and set aside;
[0032] 2. Preserve the slant-preserved Strain of Phylonotus red margin (provided by the Vegetable Institute of Jiangsu Academy of Agricultural Sciences) in a water bath at 30°C for 2 minutes, quickly pick 2 rings and transfer them to a 30mL laboratory to prepare a PDA liquid medium, and cultivate them in a shaker for 2 days ( 26°C, 120rpm / min), the concentration of spores in the bacterial liquid reaches 1×10 8 CFU / mL. Add distilled water to the prepared wheat bran at a ratio of 160mL / 100g, and bacteria solution at a ratio of 10μL / g, mix well, pack 60g / bag into 15cm*25cm aerobic bacteria bags, and incubate in an incubator at 26°C After 6 days, take out and dry to obtain high-quality bran.
[0033] The high-quality bran that obtains is carried out the men...
Embodiment 2
[0046] 1. Wash and filter out the mixed flour in the wheat bran purchased in the market, dry it, beat it into powder, pass it through a 40-mesh sieve, and sterilize it in an autoclave at 121°C for 20 minutes, and set aside;
[0047] 2. Preserve the strain of Bailing mushroom (provided by Vegetable Institute of Jiangsu Academy of Agricultural Sciences) on a slant in a 30°C water bath for 2 minutes, quickly pick 5 rings and transfer them to 30mL PDA liquid medium, and culture them in a shaker for 1 day (26°C, 120rpm / min ), the bacterial concentration reached 2×10 8 CFU / mL. Add distilled water at a ratio of 180mL / 100g and bacteria solution at a ratio of 15μL / g to the prepared wheat bran, and mix evenly. Pack 40g / bag into 15cm*25cm aerobic bacteria bags and cultivate in an incubator at 26°C. 6 days, take out and dry.
[0048] Measure with reference to the method of embodiment 1, assay result is as follows image 3 : The water absorption index, water solubility index and expansi...
Embodiment 3
[0050] 1. Wash and filter out the mixed flour in the wheat bran purchased in the market, dry it, beat it into powder, pass it through a 40-mesh sieve, and sterilize it in an autoclave at 121°C for 20 minutes, and set aside;
[0051] 2. Store the Auricularia auricula strain (provided by the Vegetable Institute of Jiangsu Academy of Agricultural Sciences) in a 30°C water bath for 2 minutes, pick 2 rings and transfer it to 30mL PDA liquid medium, and culture it in a shaker for 1 day (26°C, 120rpm / min). The concentration of bacteria solution reaches 5×10 8 CFU / mL. Add distilled water to the prepared wheat bran at a ratio of 120rL / 100g, add bacterial liquid at a ratio of 10μL / g, and mix evenly. Pack 60g / bag into 15cm*25cm full-oxygen bacteria bags and cultivate them in an incubator at 26°C for 4 day, take it out to dry.
[0052] Measure with reference to the method of embodiment 1, assay result is as follows Figure 5 : The water absorption index of fermented bran is 4.97% lower...
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