A tumor cell detection chip based on microfluidic technology

Abstract

The invention discloses a cancer early detection chip based on microfluidic technology, which includes a rapid sorting module for circulating tumor cells, a uniform mixing module for detection samples, a quantitative packaging module for mixed reagents, and a fluorescence detection module for discrete droplets, and the four modules are connected in sequence ;Using the characteristics of circulating tumor cells to carry tumor information, combined with inertial microfluidic cell sorting technology, chaotic microchannel mixing technology, and liquid drop quantitative packaging technology, a set of rapid sorting of circulating tumor cells, uniform mixing of test samples, and mixed reagents The tumor cell detection chip, which integrates four modules of quantitative encapsulation and discrete droplet fluorescence detection, realizes the rapid detection of circulating tumor cells from peripheral blood and completes the detection of tumor cells.

Description

technical field [0001] The invention relates to a tumor cell detection method and chip based on microfluidic technology, which has the advantages of accuracy, economy and speed. The invention belongs to the application of microfluidic technology in the medical field and the field of tumor cell detection. Background technique [0002] In the process of cancer pathogenesis, tumor cell metastasis is the main reason for the failure of tumor treatment and the death of cancer patients. The main way for tumor cells to transfer from primary solid tumors to other locations to form secondary tumors is through peripheral blood circulation. Clinical detection of circulating tumor cells (CTCs) in blood is of great significance for assessing cancer risk and monitoring treatment effects. European and American countries took the lead in entering the field of circulating tumor cell detection, and made breakthroughs in mechanism exploration and experimental verification, but are still in the...

AI Technical Summary

Problems solved by technology

The flow cytometry method has the advantage of being able to quantitatively detect circulating tumor cells (CTCs), but for rapid and general tumor detection, the flow cytometry detection method has related deficiencies: ① detection is expensive and difficult to popularize

But there are also deficiencies: ① The detection is unstable and susceptible to interference

Due to the instability of mRNA, the contamination of epithelial cells during sampling, the abnormal expression of tumor markers in peripheral blood, and the interference of pseudogenes, many factors can cause false positive results.

②The detection cost is expensive

Since RT-PCR technology belongs to genetic detection technology, its development is not yet perfect, and the detection cost is relatively high. The cost of a single individual detection is 8,000-10,000 yuan per time, which restricts the promotion and application of this technology.

③ Quantitative detection cannot be realized

Most instruments adopt active microfluidic technology that requires external energy field input such as electric field and magnetic field to realize rapid detection of tumor cells in whole blood samples. Compared with passive and active detection, the detection rate is faster, but the input of external energy will affect the The cells in the blood sample are destroyed and the biocompatibility is poor, which ultimately affects the detection accuracy to a certain extent

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