Fluorescent microspheres and preparation method and fluorescence coding method thereof

Abstract

The invention relates to fluorescent microspheres and a preparation method and a fluorescent coding method thereof. The preparation method comprises the following steps: mixing microspheres with a silane reagent and a solvent for activation to obtain activated microspheres, with the silane reagent being at least one of an epoxy silane reagent, a chlorosilane reagent, an isocyanate silane reagent,a butanedioic anhydride silane reagent and a chlorosulfonyl phenyl silane reagent; and carrying out a coupling reaction on the activated microspheres and fluorescent dye in a solvent to obtain the fluorescent microspheres. According to the preparation method, one or more types of fluorescent dye are coupled to surfaces of the activated microspheres, so that one or more different fluorescence signals can be obtained. Meanwhile, the mass-volume ratio of each fluorescent dye is adjusted, so that one or more microspheres with different fluorescence and different intensities can be obtained, namelyfluorescent coded microspheres are obtained. The prepared fluorescent microspheres have the advantages of large fluorescent dye coding space, high fluorescent dye intensity and the like, and the defect that the fluorescence intensity of fluorescent coded microspheres prepared by traditional preparation methods cannot meet the requirement is avoided.

Description

technical field [0001] The invention relates to the technical field of biomolecular labeling, in particular to a fluorescent microsphere and a method for its preparation and fluorescent coding. Background technique [0002] Suspension microarray technology based on fluorescent microspheres and their fluorescent codes has the ability to simultaneously screen and quantify multiple proteins and cytokines in the same sample, and has high research and application value in the field of disease diagnosis. The core technology of the suspension microarray technology is the fluorescent-encoded microspheres with unique labeling signals during the detection process. In order to make the fluorescent-encoded microspheres applicable to high-throughput multi-indicator detection systems, this requires that the fluorescent-encoded microspheres have high fluorescence intensity, Uniform size, easy surface functionalization, and good biocompatibility. [0003] The traditional preparation of flu...

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Problems solved by technology

However, the coding space of fluorescent dyes in these preparation methods is small, and the intensity of labeled fluorescent dyes is low, which cannot meet the needs of biochemical detection.

[0004] In addition, fluorescent dyes are essentially organic substances containing unsaturated bonds, which are easily oxidized by oxygen in air or water, resulting in fluorescence quenching

When fluorescent microspheres or fluorescent-encoded microspheres prepared by traditional methods are exposed to samples such as biological and aqueous solutions, the fluorescence is easily quenched due to the presence of a large number of oxygen molecules in these samples, which in turn affects the detection of fluorescent signals by the instrument.

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