One-step type embryo culture liquid
An embryo culture solution and embryo culture technology, applied in the field of one-step embryo culture solution, can solve the problems of harmful decomposition products and achieve the effects of protecting cells, promoting safety, and inhibiting the decomposition and utilization of harmful products
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Embodiment 1
[0033] One-step embryo culture medium
[0034] Material name Formula content Sodium chloride 99.937mmol / L potassium chloride 5.531mmol / L magnesium sulfate 1.0mmol / L glucose 1.0mmol / L Gentamicin 9.5mg / L Ethylenediaminetetraacetic acid 0.01mmol / L sodium pyruvate 10.51mmol / L Alanyl Glutamine 27.49mmol / L taurine 0.48mmol / L D-sorbitol 0.23mmol / L calcium chloride 1.8mmol / L Sodium dihydrogen phosphate 0.15mmol / L Potassium dihydrogen phosphate 0.15mmol / L sodium bicarbonate 0.64mmol / L (±)-α-lipoic acid 0.078mmol / L Acetyl L-Carnitine HCl or Acetyl L-Carnitine 0.078mmol / L N-acetyl-L-cysteine 0.785mmol / L 4-Hydroxyethylpiperazineethanesulfonic acid HEPES 9.225mmol / L 3-(N-Morpholinyl) propanesulfonic acid sodium salt MOPS-Na 9.165mmol / L essential amino acid 1.6mmol / L non-essential amino acids 0.9mmol / L serum albumin 0.5g / L ser...
Embodiment 2
[0036] One-step embryo culture medium
[0037] Material name Formula content Sodium chloride 89.937mmol / L potassium chloride 5.013mmol / L magnesium sulfate 0.898mmol / L glucose 0.899mmol / L Gentamicin 5.0mg / L Ethylenediaminetetraacetic acid 0.009mmol / L sodium pyruvate 9.459mmol / L Alanyl Glutamine 24.741mmol / L taurine 0.432mmol / L D-sorbitol 0.207mmol / L calcium chloride 1.621mmol / L Sodium dihydrogen phosphate 0.135mmol / L Potassium dihydrogen phosphate 0.135mmol / L sodium bicarbonate 0.576mmol / L (±)-α-lipoic acid 0.055mmol / L Acetyl L-Carnitine HCl or Acetyl L-Carnitine 0.044mmol / L N-acetyl-L-cysteine 0.702mmol / L 4-Hydroxyethylpiperazineethanesulfonic acid HEPES 8.125mmol / L 3-(N-Morpholinyl) propanesulfonic acid sodium salt MOPS-Na 8.125mmol / L essential amino acid 0.7mmol / L non-essential amino acids 0.5mmol / L serum albumin 0....
Embodiment 3
[0039] One-step embryo culture medium
[0040]
[0041]
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