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Method for detecting hydrogen peroxide and relevant target object on the basis of nanoprobe

A nano-probe and detection method technology, which is applied in the direction of measuring devices, material analysis through optical means, instruments, etc., can solve the problems of large error in detection results, cumbersome and time-consuming operations, etc.

Active Publication Date: 2019-11-05
FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional uric acid detection mainly relies on electrochemical methods, chemiluminescence methods, and spectrophotometric methods. These methods cannot avoid the interference of complex components in blood, and the detection requires pre-processing such as centrifugation and cleaning. Not only is the operation cumbersome and time-consuming, but also Causes problems such as large errors in actual test results

Method used

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  • Method for detecting hydrogen peroxide and relevant target object on the basis of nanoprobe
  • Method for detecting hydrogen peroxide and relevant target object on the basis of nanoprobe
  • Method for detecting hydrogen peroxide and relevant target object on the basis of nanoprobe

Examples

Experimental program
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Effect test

preparation example 1

[0085] Preparation of water-soluble NaCeF without oleic acid ligand by acid washing 4 :Tb 3+ Fluorescent Nanomaterials:

[0086] Take 15mL of absolute ethanol solution, add dropwise an appropriate amount of hydrochloric acid to make it pH=1; add 20mg of NaCeF 4 :Tb 3+ Nanoparticles (see figure 2 (shown) was dissolved in the above ethanol solution, ultrasonicated for 30 minutes; after centrifugation, washed several times with absolute ethanol and distilled water; the cleaning process was centrifuged at a speed of 15,000 rpm for 10 minutes, and the finally obtained nanoparticles were transparent after acid washing, and finally dispersed in 4 mL of deionized water.

[0087] Among them, NaCeF 4 :Tb 3+ Nanoparticles can be prepared by the method disclosed in the prior art, such as using the method in the literature: HLian, Y Dai, D Yang, Z Cheng, C Li, et al, Nanoscale, 2014, 6(16):9703-9712.

[0088] The prepared water-soluble NaCeF without oleic acid ligand 4 :Tb 3+ The...

Embodiment 1

[0090] The present embodiment is used for the biological detection of hydrogen peroxide, and the following are specific operation steps:

[0091] (1) Use a polystyrene 96-well plate as the carrier used for detection, and add 8 rows of 100 μL of NaCeF obtained in Preparation Example 1 to the set microwells. 4 :Tb 3+ Aqueous solution of nanoparticles, NaCeF per row 4 :Tb 3+ The concentration of the aqueous solution of nanoparticles is 200, 100, 50, 25, 10, 5, 2.5, 1 μg / mL, and 12 groups of 100 μL of hydrogen peroxide with different concentrations are added to each row, and the concentrations are 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078, 0.039, 0.019, 0μM; 12 groups of mixed solutions were obtained for each row; uniformly oscillated in a constant temperature box at 37 degrees Celsius for 2 hours, and the luminous intensity of 8 rows of 12 groups of mixed solutions was measured respectively. The luminescence quenching efficiency of the mixed solution; when the luminescence ...

Embodiment 2

[0095] This embodiment is used to generate the concentration detection of the related substances of the hydrogen peroxide system (taking the detection of uric acid as an example), and the specific operation process is as follows:

[0096] (1) Use a polystyrene 96-well plate as the carrier used for detection, and add 100 μL of NaCeF to the pre-set microwells 4 :Tb 3+ Add 50 μL of uric acid with different concentrations to the aqueous solution of nanoparticles, the concentrations are 900, 300, 100, 33.33, 11.11, 3.704, 1.234, 0.411, 0.137, 0.047, 0.015, 0 μM; then add 50 μL of 0.011U / mL uricase buffer solution (pH=8.7), to obtain 12 groups of mixed solutions; shake evenly in a thermostat at 37 degrees Celsius for 3 hours, the reaction stopped, and measure the luminous intensity of the 12 groups of mixed solutions respectively; the luminous intensity obtained by the blank group was the strongest, As the concentration of uric acid increases, the luminous intensity gradually decre...

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Abstract

The invention belongs to the technical field of biological detection, and discloses a method for detecting a hydrogen peroxide and a relevant target object on the basis of a nanoprobe. The method adopts a water-soluble rare earth doped NaCeF4 nanomaterial as a fluorescence probe, rare earth ion luminescence is quenched through the oxidation-reduction reaction of the hydrogen peroxide and a ceriumion, and the change of doped rare earth ion fluorescence intensity is used for realizing the detection of hydrogen peroxide concentration. The method can be used for detecting the hydrogen peroxide instandard solution or reactants in an enzymatic reaction for generating the hydrogen peroxide, and the detection of the hydrogen peroxide, biological enzymes or zymolyte (such as uric acid) in serumscan be realized. The method has the advantages of being convenient in operation, good in anti-interference performance, quick, sensitive, economic, practical and the like, a theoretical foundation andtechnical support are provided for solving the real-time detection of the relevant substances of uric acid and hydrogen peroxide generation systems in a complex system, and the method has certain clinic application potential.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for detecting hydrogen peroxide and related targets based on nano-probes. Background technique [0002] Hydrogen peroxide (H 2 o 2 , commonly known as hydrogen peroxide) solution is a colorless and odorless liquid, and is currently a strong oxidizing agent widely used in the paper and textile industries. However, hydrogen peroxide is banned from being added to food because it is a toxic chemical that is extremely oxidizing and corrosive. At present, the detection of hydrogen peroxide mainly adopts electrochemical methods, chromatography and spectrophotometry, etc., which have the problems of complicated process and insufficient sensitivity, and there is still a lack of simple and quick methods for its trace detection. [0003] It is worth noting that many physiological activities of the human body are related to hydrogen peroxide, such as uric acid will p...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 涂大涛雷夏莲陈学元刘易小东余少桦宫仲亮
Owner FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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