Breeding method of tomato recessive genetic male sterility maintainer line

Abstract

The invention discloses a breeding method of a tomato recessive genetic male sterility maintainer line. The method comprises the following steps that a first expression cassette for expressing a pollen fertility protein and a second expression cassette for expressing a labeled protein are introduced into a tomato recessive genetic male sterility line to obtain transgenic tomatoes, wherein sterility of the tomato recessive genetic male sterility line is caused by the function loss of the pollen fertility protein or inhibition of the expression of the pollen fertility protein, meanwhile, the transgenic tomatoes meeting the conditions A1), A2) and A3) are the tomato recessive genetic male sterility maintainer line, A1) refers to male fertility, A2) refers to expression of the labeled protein,and A3) refers to insertion of the first expression cassette and the second expression cassette in the same locus of the same chromosome of the tomato recessive genetic male sterility line; the maintainer line serves as a male parent, and the sterility line serves as a female parent for hybridization; a strain capable of expressing the labeled protein in hybrid offspring is a male fertility maintainer line, and a strain incapable of expressing the labeled protein is a sterility line. The method has important application value.

Description

technical field [0001] The invention belongs to the field of genetic breeding, and in particular relates to a breeding method for tomato recessive male sterility maintainer lines. Background technique [0002] Tomato (Solanum lycopersicum) belongs to Solanaceae tomato genus, is an important economic crop and model plant, and also the solanaceous vegetable crop with the largest planting area and high output value in Beijing. Strengthening tomato-related basic research and applied research is of great significance for promoting the construction of urban modern agriculture in Beijing. [0003] As a strictly self-pollinated crop, tomato has obvious heterosis, high hybrid uniformity and strong stress resistance, so hybrids are mainly used in production. At present, tomato hybrid seed production is mainly carried out by artificial castration. Using the male sterile line as the female parent to produce hybrid seeds can save the process of manual detasseling, reduce costs, improve...

AI Technical Summary

Problems solved by technology

Various tomato male sterile materials available in natural resources have their own advantages and disadvantages, which limit their application in hybrid seed production

Although the type with exposed stigma has difficulty in self-pollination, in the case of group planting, pollination between adjacent plants and adjacent flowers is prone to occur, so the natural self-pollination rate is relatively high. From the perspective of sterile lines, self-pollination cannot be completely avoided. possibility of delivery

The non-dehiscing type can basically avoid the possibility of self-fertilization, but because the style is shorter than the stamen anther tube, artificial pollination is still required

Stamenless or stamen-degenerated types can also completely avoid selfing, but the agronomic traits in such materials are generally poor and difficult to apply (Susan et al., 1997; Chen Yuhui et al., 2004; Xing Hucheng et al., 2004)

At present, more than 40 pollen abortion types found in natural resources have great application potential in tomato hybrid seed production, but such materials are generally controlled by a pair of recessive nuclear genes, most of which have not yet been cloned (Susan et al., 1997; Jeong et al., 2014), also limits the application

To sum up, the various male sterile materials currently available in tomato cannot be effectively used in the production of hybrid seeds, and tomato hybrid seed production is still mainly carried out by artificial emasculation.

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