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Method for establishing fingerprint of ginseng fruit medicinal material and standard fingerprint thereof

A technology of fingerprint spectrum and establishment method, which is applied to the establishment method of ginseng fruit medicinal material fingerprint spectrum and the field of standard fingerprint spectrum, can solve the problem of no legal quality control standard for medicinal materials, and achieves perfect quality control technology, easy operation, avoidance of singleness and limited effect

Active Publication Date: 2019-11-01
吉林省集安益盛药业股份有限公司
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Problems solved by technology

[0003] Ginseng fruit is the mature fruit of ginseng. Studies have shown that its active ingredients are ginsenosides, and its content is about 4 times that of the underground rhizome. A variety of drugs have been used clinically, but the Chinese Pharmacopoeia has not included the relevant content of the medicinal material of ginseng fruit, and there is currently no legal quality control standard for the medicinal material

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  • Method for establishing fingerprint of ginseng fruit medicinal material and standard fingerprint thereof
  • Method for establishing fingerprint of ginseng fruit medicinal material and standard fingerprint thereof
  • Method for establishing fingerprint of ginseng fruit medicinal material and standard fingerprint thereof

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Embodiment 1

[0066] (1) Experimental materials:

[0067] The 40 batches of ginseng fruit medicinal materials were collected from different areas of Baishan City, Jilin Province in August 2017. The specific sample information is shown in Table 1:

[0068] Table 1 Sample information of 40 batches of ginseng fruit medicinal materials

[0069]

[0070]

[0071] (2) Instruments and reagents:

[0072] High performance liquid chromatography: Waters 2695 high performance liquid chromatography (Waters, USA); Waters2996PDA diode array detector (Waters, USA); Empower chromatographic workstation (Waters, USA); METTLERAB135-S (Mettler-Torto Lido Instruments (Shanghai) Co., Ltd.); analytical acetonitrile was chromatographically pure (Sigma).

[0073] The remaining reagents were analytically pure (Beijing Chemical Reagent Factory); water was double distilled water, prepared by Millipore pure water system (Millipore, Milford, MA, USA); ginsenoside Re (Ginsenoside Re, abbreviated as Re, batch numbe...

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Abstract

The invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, and particularly relates to a method for establishing a fingerprint of a ginseng fruit medicinal material and a standard fingerprint thereof. The method for establishing the fingerprint of the ginseng fruit medicinal material provided by the invention includes the following steps of: extractingthe ginseng fruit medicinal material by using methanol, and then performing high performance liquid chromatography analysis on the obtained extracting solution, selecting a suitable mobile phase, controlling a gradient elution program, establishing an HPLC fingerprint, and combining a principal component analysis method to better evaluate the quality of the ginseng fruit medicinal material and ensure the consistency of the quality of the ginseng fruit medicinal material. The method provided by the invention is simple and convenient to operate, is accurate and reliable, can make up for the deficiencies of an existing quality control technology, better improves the quality control technology of the ginseng fruit medicinal material, avoids the singleness and limitation of quality control, provides a scientific basis for the quality control of the ginseng fruit medicinal material, and lays a foundation for improving the quality control level of the ginseng fruit medicinal material.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, in particular to a method for establishing fingerprints of ginseng fruit medicinal materials and a standard fingerprint thereof. Background technique [0002] Ginseng (Panaxginseng C.A.Meyer) is a perennial herb of the Araliaceae genus Panax. , soothe the nerves and improve intelligence, etc. It is mostly used for physical weakness, cold limbs, weak pulse, spleen deficiency, lack of food, lung deficiency, asthma and cough, thirst for body fluid wounds, internal heat and thirst, deficiency of Qi and blood, chronic illness and weakness, palpitations, insomnia, Treatment of diseases such as impotence and uterine cold. Ginseng is one of the important specialties in my country, and it is also a precious medicinal material that is well-known both at home and abroad. It is known as the "King of Herbs". Its active ingredients are mainly saponins and polysa...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/36
CPCG01N30/02G01N30/06G01N30/36
Inventor 刘建明许旭东吴海峰佟晓乐刘红董新
Owner 吉林省集安益盛药业股份有限公司
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