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Fermenting medium and method for producing spores by liquid submerged fermentation of Purpureocillium lilacinum

A liquid submerged fermentation and fermentation medium technology, applied in the field of bioengineering, to achieve the effects of shortening the fermentation cycle, promoting production and maturation, and promoting sporulation and spore maturation

Inactive Publication Date: 2019-10-25
KINGENTA ECOLOGICAL ENG GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on the control conditions of the liquid submerged fermentation technology of Paecilomyces lilacinus. Based on the different dissolved oxygen requirements in the growth stage of the bacteria and the spore formation and maturity stages, it is applied to the control of fermentation parameters to improve the production of spores. rate, there are no reports on this

Method used

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  • Fermenting medium and method for producing spores by liquid submerged fermentation of Purpureocillium lilacinum
  • Fermenting medium and method for producing spores by liquid submerged fermentation of Purpureocillium lilacinum
  • Fermenting medium and method for producing spores by liquid submerged fermentation of Purpureocillium lilacinum

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Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1: a kind of fermented medium of Paecilomyces lilacinus liquid fermentation produces spore, contains the material of following proportioning: sucrose 45g / L, soluble starch 40g / L, soybean powder 20g / L, potassium dihydrogen phosphate 4g / L L, anhydrous calcium chloride 0.1g / L, magnesium sulfate heptahydrate 0.3g / L, cobalt chloride hexahydrate 0.01g / L, ferric sulfate heptahydrate 0.05g / L, manganese sulfate monohydrate 0.005g / L, seven Zinc sulfate hydrate 0.014g / L.

Embodiment 2

[0026] Embodiment 2: a kind of fermented medium of Paecilomyces lilacinus liquid fermentation produces spore, contains the following material of proportioning: glucose 80g / L, soluble starch 23g / L, cottonseed powder 35g / L, potassium dihydrogen phosphate 2g / L L, calcium chloride anhydrous 0.4g / L, magnesium sulfate heptahydrate 0.1-g / L, cobalt chloride hexahydrate 0.037g / L, iron sulfate heptahydrate 0.01g / L, manganese sulfate monohydrate 0.016g / L, Zinc sulfate heptahydrate 0.005g / L.

Embodiment 3

[0027] Embodiment 3: a kind of fermented medium of Paecilomyces lilacinus liquid fermentation produces spore, contains the material of following proportioning: sucrose 45g / L, soluble starch 23g / L, soybean powder 20g / L, potassium dihydrogen phosphate 4g / L L, anhydrous calcium chloride 0.3g / L, magnesium sulfate heptahydrate 0.3g / L, cobalt chloride hexahydrate 0.037g / L, iron sulfate heptahydrate 0.05g / L, manganese sulfate monohydrate 0.016g / L, seven Zinc sulfate hydrate 0.014g / L.

[0028] Embodiment: 4: the impact comparison of different fermentation medium formulas on sporulation effect

[0029] (1) Fermentation medium formula:

[0030] Formula 1: glucose 80g / L, soybean powder 35g / L, potassium dihydrogen phosphate 10g, zinc sulfate 0.1g;

[0031] Formula 2: glucose 80g / L, tryptone 16g / L, potassium dihydrogen phosphate 10g, zinc sulfate 0.1g;

[0032] Formula 3: glucose 80g / L cottonseed cake powder 50g / L, potassium dihydrogen phosphate 10g, zinc sulfate 0.1g;

[0033] Formula...

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Abstract

The invention provides a fermenting medium for producing spores by liquid submerged fermentation of Purpureocillium lilacinum and a production method of the fermenting medium. The fermenting medium comprises 45-80 g / L of sucrose or glucose, 23-40 g / L of soluble starch, 20-35 g / L of soybean flour or cottonseed powder, 2-4 g / L of monopotassium phosphate, 0.1-0.4 g / L of anhydrous calcium chloride, 0.1-0.3 g / L of magnesium sulfate heptahydrate, 0.01-0.037 g / L of cobalt chloride hexahydrate, 0.01-0.05 g / L of iron sulfate heptahydrate, 0.005-0.016 g / L of manganese sulfate monohydrate, and 0.005-0.014 g / L of zinc sulfate heptahydrate. Fermenting time of the liquid submerged fermentation is 55-65 h; fermenting temperature is 26-28 DEG C; dissolved oxygen control is subjected to two-stage control;dissolved oxygen value is controlled to 45-60% in the 0-24-hour bacterial growth stage of 0-24 hours, and the dissolved oxygen value is controlled to 10-15% in the 24-65-hour spore formation and maturing stage. The fermenting cycle for producing spores by liquid submerged fermentation of Purpureocillium lilacinum is shortened evidently; spore yield is increased greatly; fermentation cost of Purpureocillium lilacinum is reduced; the produced spores can well kill root-knot nematodes.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a fermentation medium for producing spores by liquid submerged fermentation of Paecilomyces lilacinus and a method for rapidly producing spores by using the same to carry out liquid submerged fermentation of Paecilomyces lilacinus. Background technique [0002] Paecilomyces lilacinus belongs to the subphylum Deuteromycotina, Cyclosporium, Hyphospora, Polyporaceae, and Paecilomyces genus. It has outstanding performance in plant disease control and control, and is currently the main strain used in production. The main feature of Paecilomyces lilacinus is that the conidiophores are upright, phialides are usually arranged in 2-4 rounds on the spores, the base is bottle-shaped or nearly spherical, the conidia are monospore clusters, and the conidia are elliptical to spindle Shape, smooth or slightly rough wall, 2.5-3.0um*2.0-2.2um, transparent and colorless when single, purple ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12R1/79
CPCC12N1/14C12N3/00
Inventor 顾志光杨佳林孟祥坤胡兆平徐问凤林连兵
Owner KINGENTA ECOLOGICAL ENG GRP
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