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Photosynthetic bacteria strain, biocontrol agent, biocontrol fermentation liquid, preparation method and application thereof

A technology of photosynthetic bacteria and biocontrol agents, applied in the field of photosynthetic bacteria strains, to achieve the effects of no phytotoxicity of crops, short cultivation time, and environmental friendliness

Active Publication Date: 2019-10-22
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Based on this, it is necessary to provide a photosynthetic bacterial strain, a biocontrol agent, a biocontrol fermentation liquid, a preparation method and an application for the prevention and treatment of citrus canker.

Method used

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  • Photosynthetic bacteria strain, biocontrol agent, biocontrol fermentation liquid, preparation method and application thereof
  • Photosynthetic bacteria strain, biocontrol agent, biocontrol fermentation liquid, preparation method and application thereof

Examples

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preparation example Construction

[0032] A preparation method of photosynthetic bacteria biocontrol agent for the prevention and treatment of citrus canker disease, comprising the following steps:

[0033] Activation: Cultivate the preserved species of photosynthetic bacterial strains according to the plate culture method, and cultivate until a single brown colony appears;

[0034] Seed culture: the brown-red single colony is connected to the photosynthetic bacteria liquid medium for seed culture to the logarithmic growth phase to obtain the bacterial liquid;

[0035] Production culture: The bacteria solution is inoculated into the photosynthetic bacteria production medium at 5% of the total volume of the photosynthetic bacteria strain, and the photosynthetic bacteria biocontrol agent is prepared by production and cultivation to the logarithmic growth phase, such as figure 2 Shown.

[0036] When activated, the preserved species of Rhodobacter capsulata A18 strain was cultured according to the plate culture method unti...

Embodiment 1

[0051] A strain of photosynthetic bacteria, its name is Rhodobacter capsulata A18 strain.

[0052] The main biological characteristics are: culturing on a double-layer solid flat medium for 2 to 4 days to form brownish-red round colonies with neat and smooth edges and a colony diameter of 0.2mm to 0.60mm; cultured in liquid medium for 4 days, brownish red; Grows well under aerobic conditions; physiological and chemical characteristics are negative for VP reaction and methyl red reaction, H 2 S reaction, gelatin liquefaction, urease test and indole test were all positive. The optimum growth temperature was 28℃~32℃ and pH=7.0.

Embodiment 2

[0054] A photosynthetic bacteria biocontrol agent, using the Rhodobacter capsularis A18 of Example 1 as a raw material, and prepared by activation, seed culture, and production culture. The specific preparation method includes the following steps:

[0055] A photosynthetic bacteria biocontrol agent, which uses the Rhodobacter capsularis A18 strain of Example 1 as a raw material and is prepared through activation, seed culture, and production culture. The specific preparation method includes the following steps:

[0056] (1) Activation: The preserved species of Rhodobacter capsularis A18 strain is cultured according to the double-layer plate culture method, and cultivated until a single red colony appears; the medium used is a photosynthetic bacteria solid medium, and the formula is: peptone 5g / L, Glucose 1g / L, YE2.5g / L, (NH 4 ) 2 SO 4 1.8g / L, Na 2 CO 3 1.0g / L, K 2 HPO 4 0.4g / L, MgSO 4 0.5g / L, agar powder 18g / L, pH=7.0.

[0057] (2) Seed culture: Put the brown-red single colony cu...

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Abstract

The invention discloses a photosynthetic bacteria strain, a biocontrol agent, biocontrol fermentation liquid, a preparation method and an application thereof. The photosynthetic bacterial strain is rhodobacter capsulatus A18. (1) The photosynthetic bacteria rhodobacter capsulatus A18 of the invention has simple production process, short cultivation time and low cost, and the strain can be preparedby a simple, rapid and low-cost operation method to obtain the biocontrol agent and the biocontrol fermentation broth for preventing and controlling citrus canker disease. (2) The biocontrol agent and the biocontrol fermentation broth prepared by the photosynthetic bacteria rhodobacter capsulatus A18 of the invention are environmentally friendly, and non-toxic to humans and animals, have no phytotoxicity to crops, are simple and convenient to apply, and are not easy to cause drug resistance for insect disease.

Description

Technical field [0001] The invention relates to the technical field of biological pesticides, in particular to photosynthetic bacterial strains, biocontrol bacteria agents, biocontrol fermentation broths and preparation methods and applications for the prevention and treatment of citrus canker. Background technique [0002] As a fruit tree planted widely in the world, citrus has strong adaptability and is widely planted in various countries in the world. In citrus production, due to pests and diseases, huge economic losses have been brought to the citrus industry. Among them, citrus canker is one of the most dangerous diseases of citrus, and it is also one of the most important and common diseases in citrus producing areas in my country, especially in Guangdong, Guangxi, Hunan, Hubei, and Fujian provinces. Moreover, in the past ten years, the occurrence and prevalence of citrus canker has been increasing. Citrus canker, as a bacterial disease, is a citrus disease caused by Xant...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P1/00C12R1/01
CPCA01N63/00C12R2001/01C12N1/205A01N63/10
Inventor 程菊娥苏品刘勇张德咏郑立敏王忠勇周波
Owner HUNAN PLANT PROTECTION INST
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