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Preparation method of three-dimensional porous antibacterial silk fibroin/graphene/titanium dioxide bone tissue engineering scaffold

A technology of bone tissue engineering and titanium dioxide, used in tissue regeneration, pharmaceutical formulations, prostheses, etc., can solve the problems of poor antibacterial properties, unsatisfactory mechanical properties of scaffolds, and poor osteoinductive ability, and achieves promotion of dispersion and improved mechanical properties. Performance, easy-to-get results

Active Publication Date: 2019-10-22
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Purpose of the invention: In view of the problems that the mechanical properties of the RSF composite scaffold are not ideal as a scaffold for bone tissue engineering, the osteoinductive ability is not strong, and the antibacterial property is not obvious, the present invention provides a three-dimensional porous antibacterial silk fibroin / graphene / titanium dioxide bone Fabrication method of tissue engineering scaffold using rGO@TiO 2 Composite nanoparticles synergistically strengthen RSF scaffolds, which can not only improve the mechanical properties of RSF scaffolds, but also endow the scaffolds with antibacterial and osteoinductive properties

Method used

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  • Preparation method of three-dimensional porous antibacterial silk fibroin/graphene/titanium dioxide bone tissue engineering scaffold

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Embodiment 1

[0029] 1) The boiling mass percentage is 0.5%~1% sodium carbonate (Na 2 CO 3 ) aqueous solution to degumming silkworm cocoons, after degumming, dissolve it in LiBr aqueous solution with a molar concentration of 9.0±0.5 mol / L, remove impurities and LiBr through centrifugation, filtration, and dialysis to obtain RSF aqueous solution, and concentrate it to RSF mass fraction 2% solution A for use. The dialysis is to put the RSF solution after centrifugal suction filtration into a cellulose dialysis bag, after sealing, immerse in deionized water to start dialysis, the dialysis temperature is 6~12°C, the dialysis time is 72 h, and the water change cycle is 3~12 ℃. 4 h.

[0030] 2) Mix 0.5-4 mg / mL GO dispersion, 10-30% TiO 2 The hydrosol was blended with 25% by volume ethanol in a volume of 100 mL to control the concentration of GO and TiO 2 The mass ratio is 1:5, and after it is dispersed uniformly in an ultrasonic disperser, rGO@TiO is synthesized by a hydrothermal method. 2 C...

Embodiment 2

[0036] The difference between embodiment 2 and embodiment 1 is that embodiment 2 controls rGO@TiO 2 The mass ratio to RSF is 1:50, while Example 1 controls rGO@TiO 2 Be 1:100 with the mass ratio of RSF, other operations are identical with embodiment 1, as follows:

[0037] 1) The boiling mass percentage is 0.5%~1% sodium carbonate (Na 2 CO 3 ) aqueous solution to degumming silkworm cocoons, after degumming, dissolve it in LiBr aqueous solution with a molar concentration of 9.0±0.5 mol / L, remove impurities and LiBr through centrifugation, filtration, and dialysis to obtain RSF aqueous solution, and concentrate it to RSF mass fraction 2% solution A for use. The dialysis is to put the RSF solution after centrifugal suction filtration into a cellulose dialysis bag, after sealing, immerse in deionized water to start dialysis, the dialysis temperature is 6~12°C, the dialysis time is 72 h, and the water change cycle is 3~12 ℃. 4 h.

[0038] 2) Mix 0.5-4 mg / mL GO dispersion, 10-3...

Embodiment 3

[0044] The difference between embodiment 3 and embodiment 2 is that embodiment 3 controls rGO@TiO 2 The mass ratio to RSF is 1:20, while Example 2 controls rGO@TiO 2 The mass ratio with RSF is 1:50, and other operations are identical with embodiment 2, as follows:

[0045] 1) The boiling mass percentage is 0.5%~1% sodium carbonate (Na 2 CO 3 ) aqueous solution to degumming silkworm cocoons, after degumming, dissolve it in LiBr aqueous solution with a molar concentration of 9.0±0.5 mol / L, remove impurities and LiBr through centrifugation, filtration, and dialysis to obtain RSF aqueous solution, and concentrate it to RSF mass fraction 2% solution A for use. The dialysis is to put the RSF solution after centrifugal suction filtration into a cellulose dialysis bag, after sealing, immerse in deionized water to start dialysis, the dialysis temperature is 6~12°C, the dialysis time is 72 h, and the water change cycle is 3~12 ℃. 4 h.

[0046] 2) Mix 0.5-4 mg / mL GO dispersion, 10-3...

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Abstract

The invention relates to a preparation method of a three-dimensional porous antibacterial silk fibroin / graphene / titanium dioxide bone tissue engineering scaffold, comprising the following steps: 1) degumming silkworm cocoons and carrying out subsequent treatment to obtain a 1-4% aqueous solution of RSF; 2) ultrasonically dispersing a graphite oxide (GO) dispersion, titanium dioxide (TiO2) hydrosoland ethanol and blending, synthesizing rGO(at)TiO2 composite nanoparticles by a hydrothermal method, and washing and drying to obtain a powder; 3) ultrasonically dispersing the rGO(at)TiO2 powder inwater, and blending the rGO(at)TiO2 powder into the aqueous solution of RSF in 1); 4) concentrating the mixed solution in 3) until the mass percentage of RSF is 4-15%; 5) adding the aqueous solution of ethanol to the mixed solution of RSF / rGO(at)TiO2, carrying out mechanical stirring, and standing to form a gel; and 6) superfreezing the gel and freeze-drying to prepare the RSF / rGO(at)TiO2 three-dimensional porous tissue engineering scaffold. By using the rGO(at)TiO2 composite nanoparticles for synergetic enhancement of the RSF material, mechanical properties of the RSF scaffold are improved, and the scaffold also can be endowed with antibacterial property, osteoinductive capability and other functions.

Description

technical field [0001] The invention relates to the fields of tissue engineering and biomedical materials, in particular to a method for preparing a three-dimensional porous antibacterial silk fibroin / graphene / titanium dioxide bone tissue engineering scaffold. Background technique [0002] According to recent surveys, the number of people with bone defects caused by traffic accidents or orthopedic diseases in my country exceeds 3 million people every year, and the current effective method for clinical treatment of bone defects is to implant bone tissue engineering scaffolds into the defect, by simulating extracellular matrix and Construct a suitable microenvironment to induce bone cell growth, proliferation and differentiation. [0003] Regenerated silk fibroin (RSF) is a natural polymer material with a wide range of sources and low price, and has the advantages of good biocompatibility, degradability and low inflammatory response. Compared with other similar natural polymer...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/52A61L27/54A61L27/44
CPCA61L27/443A61L27/446A61L27/52A61L27/54A61L27/56A61L2300/102A61L2300/108A61L2300/404A61L2300/412A61L2300/45A61L2300/624A61L2430/02C08L89/00
Inventor 张超叶玮柳森刘静静丁红燕王心如
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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