Method for artificially cultivating batryticated silkworms based on Beauveria bassiana

Abstract

The invention provides a method for artificially cultivating batryticated silkworms based on Beauveria bassiana. The method is characterized in that batryticated silkworms artificially cultivated arecylindrical in shape and wrinkled and flexural, each batryticated silkworm is 4.30+ / -0.35 cm in length and 0.5+ / -0.1 cm in diameter, ten thousands of batryticated silkworms weigh 7-8 kg, milky white powdery mycelia and conidia are present on the surface of the batryticated silkworms, the batryticated silkworm are stiff and brittle, their ends are flat and smooth, the batryticated silkworms are inbright amber color, silk gland rings are visualized, and the content of ammonium oxalate tested through high-performance liquid chromatography is 6.00-6.20%; the batryticated silkworms are made by means of preparing a spore suspension inoculum, cultivating Beauveria bassiana, preparing an infected spore liquid, and preparing Beauveria bassiana spores for artificial production of batryticated silkworms. The method herein has the advantages that the method is simple and has low requirement on the production environment and production equipment, production cost is reduced greatly, pollution by sundry bacteria from the production is relieved, separating spores and a culture substrate after drying is not required, subpackaging directly to obtain products is available, nearly all spores are recyclable, the method is very suitable for rapid production of Beauveria bassiana in small-sized enterprises and production institutes and can provide improved economic benefit and social benefit.

Description

technical field [0001] The invention relates to a method for cultivating Beauveria bassiana, in particular to a method for artificially cultivating Beauveria bassiana. Background technique [0002] white silkworm ( Bombyx batryticatus ), also known as Bombyx mori, Bombyx mori, are naturally infected (or artificially inoculated) with Beauveria bassiana ( Beauveria ) and dead dry whole worm, white silkworm as a traditional Chinese medicine, used to treat a variety of diseases, has a medicinal history of thousands of years in China, and is also widely used in Japan and Korea. Modern pharmacological research has found that the silkworm has a variety of active pharmaceutical ingredients, which can inhibit bacteria, anticonvulsant, anticoagulant, antithrombotic, promote fibrinolysis, inhibit tumor, hypnotize, lower lipid, lower blood sugar, etc. Modern medical practice research shows that the silkworm has a therapeutic effect on the treatment of mumps, epilepsy and sequelae of ...

AI Technical Summary

Problems solved by technology

Especially in recent years, research has found that the silkworm has the function of stimulating the adrenal cortex and health care, which greatly broadens the scope of use of the silkworm, and the consumption has increased sharply, resulting in a continuous rise in its price.

In addition to purchasing a large number of silkworms from various sericulture areas in my country every year, our country also produces white silkworms professionally in factories, but this is also difficult to meet the needs of the medical department.

At present, some domestic enterprises artificially infect and produce B. larvae. There are several problems: (1) The stability of the strain is not high, and most of them are spores eluted directly from natural or artificially infected B. larvae for reinoculation , the strains are not purified and optimized, and the pathogenicity is affected by many factors such as environment and climate. The time from inoculation to death of infected silkworms is not concentrated, the infection rate of silkworms is low, the yield of silkworms is low, and the ossification time is not uniform , the quality is unstable, and the yield is low; (2) Due to the restriction of the preservation method of Beauveria bassiana spore powder, the number of live spores and spore vigor will decrease significantly with the increase of time. Therefore, in actual production, there are often post-inoculation silkworm Due to the low incidence rate and long duration of the disease, the silkworms cannot die as scheduled before the silkworms are matured, causing great economic losses to the silkworm production.

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