Method for rapid aseptic separation of edible fungi haploids

Abstract

The invention discloses a method for rapid aseptic separation of edible fungi haploids. The method for rapid aseptic separation of the edible fungi haploids includes the following specific steps thatfirst, mycelia is placed in a fluid nutrient medium for culturing to obtain mature mycelium; second, a staining mycelium solution or staining protoplast suspension is prepared; third, the sorting parameters of diploids and haploids are determined; and fourth, hyphae haploids or protoplast haploids are separated and selected to obtain. The method for rapid aseptic separation of the edible fungi haploids has the advantages of overcoming the technical problems of low separation and selection rate of traditional haploids and long cultivation period. The cultivation process of the method for rapidaseptic separation of the edible fungi haploids can realize aseptic separation, avoid the haploid infection and has a short cultivation period at the same time, the haploids obtained through separation has a high yield and can be directly used as the raw material for cross breeding, especially suitable for haploids separation of mushroom, mushroom, fungus and other diploid edible fungi, and lay afoundation for the cultivation of new edible fungi.

Description

technical field [0001] The invention relates to edible fungus breeding technology, in particular to an aseptic rapid separation method of edible fungus haploids. Background technique [0002] Edible mushrooms are delicious, nutritious, and have high edible value. With the improvement of people's living standards, the people's consumption demand for edible mushrooms is increasing, and the requirements for edible mushroom species are becoming more and more diverse. There are more than 30 edible mushroom cultivation species in Henan alone. Therefore, there is an urgent need to develop and breed new edible mushroom varieties to fill the market gap, meet the needs of market development, and meet the consumption needs of the public. [0003] Edible fungi breeding technologies mainly include hybrid breeding, mutation breeding, transgenic breeding, etc. Hybrid breeding is the fastest, most effective and most widely used breeding method in the production of edible fungi. In hybrid b...

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Problems solved by technology

[0003] Edible fungus breeding techniques mainly include hybrid breeding, mutation breeding, transgenic breeding, etc. Hybrid breeding is the fastest, most effective and most widely used breeding method in the production of edible fungi. In hybrid breeding, obtaining a large number of haploids is the basis of hybrid breeding. The existing ways to obtain haploids of edible fungi mainly include single spore isolation and protoplast sorting. Single spore isolation includes spore collection, multiple gradient dilution, coating , cultivation, selection, identification and other steps to cultivate monokaryotic hyphae with cell walls (i.e. hyphae haploid), the whole cultivation cycle takes about 25 days, which takes a long time and the sorting rate is low; protoplast sorting includes hyphae Human body culture, preparation of protoplasts, transfer culture, manual picking of colonies, regeneration culture, microscopic examination, and finally mononuclear protoplasts without cell walls (that is, protoplast haploids), the workload of manual picking during the entire cultivation process Large, the success rate of picking is low, so that the haploid yield after regeneration culture is only 30% to 60%, which is far from meeting the needs of cultivating new varieties of edible fungi

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