Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Micro-cuttage and non-tube rootage technique for tissue subculture rootless test-tube plantlets of lonicera edulis lurcz

A test-tube seedling and exogenous rooting technology, which is applied in the field of micro-insertion bottle exo-rooting of T. chinensis tissue culture sub-generation rootless test-tube seedlings, can solve the problems of poor rooting development, difficulty in large-scale and rapid propagation, and transplantation survival rate of T. chinensis tissue culture seedlings Low problems such as reducing aseptic operation and medium production materials and energy consumption, diluting cultivation costs, and high transplant survival rate

Pending Publication Date: 2019-07-12
黑龙江伊蓝生物科技有限公司
View PDF12 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technique of rooting out of the bottle by micro-cuttage of the tissue-cultured plantlets of L. indigo plantlets provided by the present invention solves the technical problems that the rooting in the bottle of the tissue-cultured plantlets of L. indigo fruit is poorly developed, the survival rate of transplantation is low, the operation procedures are cumbersome, and the cultivation period is long. , slow reproduction speed, low reproduction coefficient, high cultivation cost, difficult to scale rapid breeding

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0017] [Specific embodiments] The specific embodiments of the present invention will be described in further detail below.

[0018] (1) Transitional seedling hardening: Gently remove the subcultured clustered rootless test-tube seedlings that have not entered the single plant rooting culture in the tissue culture room together with the culture bottle from the culture rack, quickly move them to the solar greenhouse, and temper under natural astigmatism. Seedlings to enhance the adaptability of rootless test tube seedlings to the environment. The temperature is controlled at 18°C ​​to 25°C, spray mist around the culture bottle at noon every day to moisturize and cool down, seal the seedlings for 1d to 3d, loosen the cover for 1d to 2d, and open the cover for 2h to 5h.

[0019] (2) Make a substrate bed: In the greenhouse, plan the substrate bed according to the north-south direction, the bed width is 110cm (the cutting bed surface is 100cm, and a 5cm protective belt is left on ea...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a seedling cultivation technique, in particular to a micro-cuttage and non-tube rootage technique for tissue subculture rootless test-tube plantlets of lonicera edulis lurcz,and belongs to the technical field of plants. The technical problems in tube rootage of the tissue subculture plantlets of the lonicera edulis lurcz, including maldevelopment, low survival rate of transplantation, complicated operation procedure, long cultivation period, low reproduction speed, low reproduction coefficient, high cultivation cost and difficulty in large-scale rapid reproduction. The micro-cuttage and non-tube rootage technique includes transitional acclimatization of the tissue subculture rootless test-tube plantlets of the lonicera edulis lurcz, preparation of a medium bed, micro-body cuttage, post-cuttage management, multiplication by the plantlets, transplanting and breeding, and the like. The micro-cuttage and non-tube rootage technique is suitable for large-scale rapidreproduction of the tissue subculture plantlets of the lonicera edulis lurcz.

Description

[0001] 【Technical field】 [0002] The invention relates to a nursery stock cultivation technology, in particular to the technology of exogenous rooting in micro-inserted vases for subcultured rootless test-tube seedlings of L. indigo fruit, belonging to the plant [technical field]. [0003] 【Background technique】 [0004] Indigo Honeysuckle ( Lonicera edulis Lurcz), also known as blue indigo fruit, black blind fruit, etc., is a small perennial deciduous shrub of Lonicera genus Lonicera, 1.0-1.5 m high, often in sunny and humid places such as forest edges, forests, shrubs, grasslands, and watersides. companion. Rich in vitamins, organic acids, sugars, flavonoids, minerals and various trace elements, it can be eaten fresh or processed into fruit juice, jam, fruit wine, etc. It has the effects of clearing away heat and detoxification, improving liver detoxification function, anti-tumor, anti-fatigue, lowering blood fat, stabilizing blood pressure, and promoting physical develop...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01G2/10A01G31/00A01G17/00
CPCA01G2/10A01G31/00A01G17/005
Inventor 韦庆和史福忠于海鸥刘桂森鲍淮
Owner 黑龙江伊蓝生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products