Micro-cuttage and non-tube rootage technique for tissue subculture rootless test-tube plantlets of lonicera edulis lurcz
A test-tube seedling and exogenous rooting technology, which is applied in the field of micro-insertion bottle exo-rooting of T. chinensis tissue culture sub-generation rootless test-tube seedlings, can solve the problems of poor rooting development, difficulty in large-scale and rapid propagation, and transplantation survival rate of T. chinensis tissue culture seedlings Low problems such as reducing aseptic operation and medium production materials and energy consumption, diluting cultivation costs, and high transplant survival rate
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[0017] [Specific embodiments] The specific embodiments of the present invention will be described in further detail below.
[0018] (1) Transitional seedling hardening: Gently remove the subcultured clustered rootless test-tube seedlings that have not entered the single plant rooting culture in the tissue culture room together with the culture bottle from the culture rack, quickly move them to the solar greenhouse, and temper under natural astigmatism. Seedlings to enhance the adaptability of rootless test tube seedlings to the environment. The temperature is controlled at 18°C to 25°C, spray mist around the culture bottle at noon every day to moisturize and cool down, seal the seedlings for 1d to 3d, loosen the cover for 1d to 2d, and open the cover for 2h to 5h.
[0019] (2) Make a substrate bed: In the greenhouse, plan the substrate bed according to the north-south direction, the bed width is 110cm (the cutting bed surface is 100cm, and a 5cm protective belt is left on ea...
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