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Fingerprint spectrum detection method for ixeris chinensis medicinal material and fingerprint spectrum of ixeris chinensis medicinal material

A technology of fingerprints and detection methods, which is applied in the field of fingerprint detection methods and fingerprints of the medicinal material C. chinensis, achieving the effects of good reproducibility, high precision and good stability

Active Publication Date: 2019-10-01
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are relatively few reports on the research on the fingerprints of C.

Method used

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  • Fingerprint spectrum detection method for ixeris chinensis medicinal material and fingerprint spectrum of ixeris chinensis medicinal material
  • Fingerprint spectrum detection method for ixeris chinensis medicinal material and fingerprint spectrum of ixeris chinensis medicinal material
  • Fingerprint spectrum detection method for ixeris chinensis medicinal material and fingerprint spectrum of ixeris chinensis medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Establishment of the Fingerprint Spectrum of the Medicament

[0038] 1 Instruments and experimental materials

[0039] 1.1 Reagent

[0040]The herbs used in the experiment were collected from various producing areas in Guangxi. Take the medicinal materials of A. chinensis, remove weeds, branches, soil and other sundries and moldy medicinal materials, keep the medicinal parts such as flowers, leaves, stems, roots, etc., and crush them into coarse powder (through No. 2 sieve). The sample was identified by Professor Wei Songji from the School of Pharmacy, Guangxi University of Traditional Chinese Medicine, as the genus of Asteraceae, Asteraceae. See Table 1 for origin.

[0041]

[0042] 1.2 Reagents

[0043] The reference substances and reagents used in the experiment are shown in Table 2-1~Table 2-2.

[0044]

[0045]

[0046] 1.3 Instruments

[0047] The instruments used in the experiment are shown in Table 3

[0048]

[0049] 2 Experimental ...

Embodiment 2

[0117] A method for detecting the fingerprints of a medicinal material of the basilisk, comprising the following steps:

[0118] (1) Preparation of mixed reference solution: Accurately weigh 11.59mg of chlorogenic acid reference substance, 6.72mg of caffeic acid reference substance, and 13.60mg of luteolin reference substance, put them in 25ml measuring bottles respectively, add appropriate amount of methanol to ultrasonically dissolve and Dilute to the mark, shake well, and then accurately measure 1.5ml of the above-mentioned chlorogenic acid stock solution, 1.5ml of caffeic acid stock solution and 6.0ml of luteolin stock solution, put them in the same 10ml measuring bottle and dilute to volume with methanol, shake well, The mixed reference solution was obtained; each 1ml of the prepared mixed reference solution contained 66.9 ug of chlorogenic acid, 40.35 ug of caffeic acid, and 306.78 ug of luteolin;

[0119] (2) Preparation of the test solution: Take 2g of the sample of Ku...

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Abstract

The invention relates to the technical field of fingerprint spectrums of traditional Chinese medicinal materials, and in particular to a fingerprint spectrum detection method for an ixeris chinensis medicinal material and a fingerprint spectrum of the ixeris chinensis medicinal material. The method comprises the following steps of (1) preparation of mixed reference solution; (2) preparation of test solution; (3) HPLC detection; (4) establishment of a contrast fingerprint spectrum; and (5) quality control of the fingerprint spectrum. By utilizing the fingerprint spectrum detection for the ixeris chinensis medicinal material and the fingerprint spectrum of the ixeris chinensis medicinal material, the quality of the ixeris chinensis medicinal material can be monitored and the authenticity ofthe ixeris chinensis medicinal material can be distinguished, so that the reality, security, effectiveness, stability and consistency of the medicinal material are ensured, a comprehensive evaluationmethod is provided for the quality control of the ixeris chinensis, scientific basis is provided for the resource development of the ixeris chinensis medicinal material, and a quality evaluation system of the ixeris chinensis medicinal material is completed.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, in particular to a method for detecting the fingerprints of the medicinal material C. chinensis and the fingerprints thereof. Background technique [0002] Although traditional Chinese medicine has a history of thousands of years of application and development, how to effectively evaluate the quality of Chinese medicinal materials has always been the focus and difficulty of research and application of Chinese medicinal materials. With the development of science and technology, fingerprint quality control technology has been proposed in the field of traditional Chinese medicine and has been widely recognized, and its application in the quality control of traditional Chinese medicine is also increasing; The atlas that can indicate the characteristics of the Chinese herbal medicine obtained by certain analytical means and testing instruments is one of ...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/86
CPCG01N30/8631G01N30/8686G01N30/88G01N2030/8809
Inventor 梁爽朱华赵立春黄健军卢森华刘亦真吴秀彩
Owner GUANGXI UNIV OF CHINESE MEDICINE
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