Application of high-temperature-resistant pleurotus ostreatus in production of ergothioneine

A technology of Pleurotus pellagra and ergothioneine, applied in the direction of microorganisms, microorganisms, fungi, etc., can solve the problems of slow growth and long fermentation time, and achieve the goals of reducing energy consumption, high yield, and shortening fermentation time Effect

Active Publication Date: 2019-09-27
弘恒泰(天津)科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although a variety of fungal and mycobacterial sources are available for ergothioneine extraction, current research has focused on the production of ergothioneine by macrofungal species such as Pleurotus pleurotus, Pleurotus pulmonica, wild Pleurotus pleurotus, Pleurotus rugosa and monkey Head mushrooms, etc., but most of the fungi are suitable for low temperature growth, and the growth is slow and the fermentation time is long

Method used

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  • Application of high-temperature-resistant pleurotus ostreatus in production of ergothioneine
  • Application of high-temperature-resistant pleurotus ostreatus in production of ergothioneine
  • Application of high-temperature-resistant pleurotus ostreatus in production of ergothioneine

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preparation example Construction

[0060] The preparation method of PDA slope medium is: take 200g peeled potatoes, cut into small pieces and put them into the pot, add 980ml water, heat to boiling on the heater, keep for 20min, filter on the measuring cup with 2 layers of gauze while it is hot. Obtain the filtrate, discard the filter residue, add 20g glucose and 25g agar to the filtrate, heat and melt it to make the volume to 1000mL, the pH value is natural, and dispense the corresponding 7mL / test tube or 70mL / eglanto bottle into test tubes or eggplant bottles, stopper Put on the rubber stopper, autoclave at 121±1℃ for 30 minutes, then spread the slope for use.

[0061] The RSL-HM2 strain grows faster on PDA medium (potato dextrose agar medium). A piece of mycelium grows for 10 days under dark conditions at 32℃. The diameter of the colony is about 80mm. The hyphae are white and aerial hyphae. Lush and light brown on the back, the colony shape is like figure 1 Shown. Under the microscope, the hyphae showed a dens...

Embodiment 1

[0069] When the solid substrate is the basic fermentation medium, the basic fermentation medium is 45g maltodextrin, 20g casein peptone, 4g potassium dihydrogen phosphate, 2g magnesium sulfate heptahydrate, 1g ammonium dihydrogen phosphate, 1g cysteine, 1g A mixture of methionine and 1mg vitamin B1.

Embodiment 2～13

[0071] When the solid substrate is a carbon source optimized medium, the carbon source optimized medium is 45g carbon source, 20g casein peptone, 4g potassium dihydrogen phosphate, 2g magnesium sulfate heptahydrate, 1g diammonium phosphate, 1g cysteine, A mixture of 1g methionine and 1mg vitamin B1, the carbon sources are: glycerin, glucose, fructose, galactose, mannitol, sorbitol, lactose, sucrose, maltose, maltodextrin, dextrin, starch or corn flour, see table for details 3.

[0072] table 3

[0073] Example Carbon source Example 1 glycerin Example 2 glucose Example 3 fructose Example 4 Galactose Example 5 Mannitol Example 6 Sorbitol Example 7 lactose Example 8 sucrose Example 9 maltose Example 10 Maltodextrin Example 11 dextrin Example 12 starch Example 13 corn flour

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Abstract

The invention discloses an application of high-temperature-resistant pleurotus ostreatus in production of ergothioneine. The pleurotus ostreatus is collected in the CGMCC (China General Microbiological Culture Collection Center) of the CCCCM (China Committee for Culture Collection of Microorganism) on October 18, 2018, with the collection number of CGMCC:16378. When applied to production of ergothioneine, the high-temperature-resistant pleurotus ostreatus has the advantages as follows: 1, the high-temperature-resistant pleurotus ostreatus shortens the fermentation time of the ergothioneine; 2, the highest culture temperature of the ergothioneine in the fermentation production process can reach 32 DEG C, energy consumption caused by culture temperature decrease can be reduced, and the production cost can be reduced; 3, the yield of the ergothioneine is higher, the production and preparation costs of the ergothioneine are reduced, and a solid foundation is laid for industrial production and commercial application of the ergothioneine.

Description

Technical field [0001] The invention belongs to the technical field of high temperature resistant Pleurotus ostreatus, and specifically relates to the application of high temperature resistant Pleurotus ostreatus in the production of thioneine. Background technique [0002] Ergothioneine is a rare and natural chiral amino acid with strong antioxidant function. Compared with other antioxidants, it has more stable physical, chemical and biochemical properties. Ergothioine has the functions of scavenging free radicals, maintaining DNA biosynthesis, detoxification, anti-radiation, cellular immunity and energy regulation in the organism. It can be expected that ergothioine has important application value in cosmetics, food and biomedicine industries. . [0003] Thioine is a natural compound produced by actinomycetes such as Mycobacterium smegmatis and filamentous fungi such as Neurospora crassa. It has recently been discovered that the fission yeast Schizosaccharomyces pombe can also p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04C12N1/14C12R1/645
CPCC12P13/04C12N1/145C12R2001/645
Inventor 孟昭晖
Owner 弘恒泰(天津)科技发展有限公司
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