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Eimeria tenella SO7-IL-2 protein and preparation method thereof

A technology of SO7-IL-2 and Eimeria, which is applied in the field of genetic engineering, can solve the problems of drug-resistant insect strains, decreased egg production rate, and harm to the chicken industry, so as to reduce the degree of disease and achieve good results. strong protective effect

Pending Publication Date: 2019-09-20
JILIN UNIV
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Problems solved by technology

Because the disease can cause the growth and development of the chicken body, the drop of egg production rate and death, etc., it will bring huge harm and loss to the chicken industry.
Drug prevention is currently the most effective measure to control coccidiosis in production, but long-term use of drugs to control coccidiosis will lead to many problems such as drug-resistant strains and drug residues

Method used

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  • Eimeria tenella SO7-IL-2 protein and preparation method thereof
  • Eimeria tenella SO7-IL-2 protein and preparation method thereof
  • Eimeria tenella SO7-IL-2 protein and preparation method thereof

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Effect test

Embodiment 1

[0025] Preparation of recombinant yeast protein pPICZαA-S07-IL-2:

[0026] According to GenBank SO7, the chicken IL-2 gene sequence and the multiple cloning site sequence of the cloning vector pMD18-T and the expression vector pPICZαA, the Primer5.0 software was used to design primers for the amplification of the SO7 and IL-2 genes, and then SO7 A linker (GGTGGAGGCGGTTCAG) was added to the upstream of the gene and the downstream of the IL-2 gene. See Table 1 for the synthetic primers of SO7 and IL-2 gene sequences.

[0027] Table 1 Primer sequences

[0028]

[0029] The target gene SO7-IL-2 ( figure 1 ) purified with pMD18-T vector and carried out enzyme digestion and sequencing identification ( figure 2 ), connect the target gene SO7-IL-2 to the yeast vector pPICZαA, and electrotransform the ligated product into X33 competent cells, mix 10 μg of the linearized plasmid linearized by SacI and 100 μL of yeast competent cells, and mix gently; ice bath After 5 min, transfe...

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Abstract

The invention provides Eimeria tenella SO7-IL-2 protein and a preparation method thereof. The Eimeria tenella SO7-IL-2 protein based a Pichia pastoris vector is novel Eimeria tenella recombinant yeast protein. Chickens are immunized by protein obtained by recombinant yeast protein total crushing and induction expression, and the recombinant protein extracted by the total crushing method is high in protecting effect. By detecting humoral immunity and cellular immunity level, the results show that the immunity level of the chickens immunized by the protein obtained by recombinant yeast protein total crushing and induction expression is evidently higher than that of control, and the protein can be used for preventing Eimeria tenella diseases.

Description

technical field [0001] The invention provides an Eimeria tenella SO7-IL-2 protein and a preparation method thereof, which is based on the Eimeria tenella SO7-IL-2 protein carried by Pichia pastoris, and is a new type of Eimeria tenella SO7-IL-2 protein. The recombined yeast protein of coccidia mesa is used for the prevention of coccidiosis lentilis and belongs to the technical field of genetic engineering. Background technique [0002] Eimeria tenella is an important parasite of chickens. Apicomplexan parasites in the cells mainly parasitize in the cecum of chickens, which can cause weight loss, serious intestinal lesions and death of chickens, and seriously harm Development of the poultry industry. The disease occurs in chicks aged 15-50 days, the incidence rate is as high as 50%-70%, and the mortality rate is about 20%-30%. Estimates of the annual global economic loss from Eimeria tenaea are approximately US$ 3 billion. Because the disease can cause the growth and devel...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/11C12N15/81A61K39/012A61P33/02C12R1/84
CPCA61K39/012A61P33/02C07K14/455C07K14/55C07K2319/00C12N15/11C12N15/815
Inventor 张西臣陈艳红宫鹏涛赵权李建华王晓岑张楠李新李赫杨举
Owner JILIN UNIV
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