Method for extracting flavone substances in Camellia nitidissima

An extraction method, Camellia japonica technology, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problems of unfavorable development and utilization of camellia japonica, low scavenging ability, low extraction rate, etc., and achieve the improvement of comprehensive utilization value, anti-oxidation Good ability, good effect of clearing ability

Inactive Publication Date: 2019-09-06
QIANNAN NORMAL UNIV FOR NATTIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the extraction rate of camellia camellia is low, and the flavonoids extracted have poor antioxidant capacity and weak scavenging ability, which is not conducive to the development and utilization of camellia camellia
[0004] In summary, the prior art has the technical problems of low extraction rate of flavonoids in Camellia japonica, poor antioxidant capacity and low scavenging ability of extracted flavonoids, which are not conducive to the development and utilization of Camellia japonica

Method used

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  • Method for extracting flavone substances in Camellia nitidissima
  • Method for extracting flavone substances in Camellia nitidissima
  • Method for extracting flavone substances in Camellia nitidissima

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0155] Example 1. A method for extracting flavonoids in golden camellia, prepared according to the following steps:

[0156] a. After drying the picked fresh leaves of Camellia chrysantha at 100°C for 20 minutes, crush them to 50 meshes, store them at 2°C to obtain product A;

[0157] b, product A is extracted with ethanol, the ethanol volume fraction is 50% during extraction, the solid-to-liquid ratio of product A to ethanol is 1:10g / mL, the extraction temperature is 40°C, and the extraction time is 40min, to obtain Golden camellia flavonoid extract.

Embodiment 2

[0158] Example 2. A method for extracting flavonoids in golden camellia, prepared according to the following steps:

[0159] a. Dry the picked fresh leaves of Camellia japonica at 105°C for 30 minutes, crush them to 60 meshes, store them at 3°C ​​at low temperature, and obtain product A;

[0160] b, product A is extracted with ethanol, the ethanol volume fraction is 60% during extraction, the solid-to-liquid ratio of product A to ethanol is 1:20g / mL, the extraction temperature is 50°C, and the extraction time is 50min, to obtain Golden camellia flavonoid extract.

Embodiment 3

[0161] Example 3. A method for extracting flavonoids in golden camellia, prepared according to the following steps:

[0162] a. Dry the picked fresh leaves of Camellia japonica at 110°C for 40 minutes, crush them to 50 mesh, store them at 2°C at low temperature, and obtain product A;

[0163] b, product A is extracted with ethanol, the volume fraction of ethanol is 70% during extraction, the solid-liquid ratio of product A to ethanol is 1:30g / mL, the extraction temperature is 60°C, and the extraction time is 60min, to obtain Golden camellia flavonoid extract.

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Abstract

The invention discloses a method for extracting flavone substances in Camellia nitidissima. The method includes the steps of a, drying the Camellia nitidissima, and crushing to obtain a product A; b,extracting the product A with ethanol to obtain Camellia nitidissima flavone substance extract, wherein the volume fraction of the ethanol during extraction is 50-90%, the solid-liquid ratio of the product A to the ethanol is 1:(10-50)g/mL, extraction temperature is 40-80 DEG C, and extraction time is 40-80 minutes. The method is high in Camellia nitidissima flavone substance extraction rate, theextraction rate of the optimal extraction process is 5.99%, the extracted flavone substances are good in oxidation resistance and good in eliminating ability, the DPPH eliminating rate of the extracted flavone substances can reach 80%, the ABTS eliminating rate of the extracted flavone substances can reach 85%, and the method is of great significance to the increasing of the additional value of the Camellia nitidissima and can provide a certain theoretical foundation for the further increasing of the comprehensive utilization value of the Camellia nitidissima.

Description

technical field [0001] The invention relates to a method for extracting flavonoids, in particular to a method for extracting flavonoids in camellia japonica. Background technique [0002] Camellia nitidissima C.W.Chi, belonging to the family Theaceae and Camellia genus, is extremely rare. 90% of wild camellias in the world are only distributed in Lanshan, Fangchenggang City, Guangxi, and Luodian, Duyun City, Guizhou; the national first-class protection One of the plants, it is called the magical oriental magic tea abroad, and is known as the queen of the tea family "the giant panda in the plant kingdom". [0003] Golden camellia tea leaves are rich in flavonoids, tea polyphenols and tea polysaccharides, which have anti-oxidation, lowering blood sugar, lowering blood pressure, anti-tumor, protecting liver and gallbladder, lowering cholesterol, dilating coronary blood vessels, enhancing heart contraction, and reducing heart beat It has high ornamental value and medicinal valu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61P39/06
CPCA61K36/82A61K2236/15A61K2236/17A61K2236/333A61P39/06
Inventor 周才碧宋丽莎周才元刘丽明石悦周新杨璐陶旭王佳菜
Owner QIANNAN NORMAL UNIV FOR NATTIES
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