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Piglet feed including bacteriostatic yeast culture, and preparation method and applications thereof

A technology of yeast culture and piglet feed, which is applied in the field of piglet feed and its preparation, can solve problems such as insufficient in-depth research, and achieve the effects of improving growth performance, increasing breeding efficiency, and reducing the death rate

Inactive Publication Date: 2019-09-06
北京中农弘科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yeast culture also contains many "unknown growth factors", and there are many reports about the positive effect of yeast culture on piglet diarrhea in Chinese and foreign academic circles, but there are not many specific in-depth studies

Method used

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  • Piglet feed including bacteriostatic yeast culture, and preparation method and applications thereof
  • Piglet feed including bacteriostatic yeast culture, and preparation method and applications thereof
  • Piglet feed including bacteriostatic yeast culture, and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Separation and screening of Saccharomyces cerevisiae HKB-36

[0030] (1) Medium: wort medium. Take 500g of malt, crush it and put it into a beaker, add 2000mL of distilled water, stir in a water bath at 45°C for 30min, increase the temperature to 70°C, and keep it for 1h; filter through 8 layers of gauze, and wash the beaker and filter tank with willow tree. When the filtrate reaches 2000mL, boil it again, cool it, and store it at 10°C for later use.

[0031] (2) Separation of yeast: take beer, wine, kimchi, fermented fruit and vegetable juice, dough and kumiss, etc. as raw materials, respectively collect 400mL or 400g raw material samples (mashed solids), place them in sterile Erlenmeyer flasks, Cover with 8 layers of gauze and ferment naturally at 25°C-30°C for 2-3d. Under aseptic conditions, shake the fermentation culture liquid sample evenly, use a sterile pipette to draw an appropriate amount of fermentation liquid into the sterilized wort culture liqui...

Embodiment 2

[0040] The preparation of embodiment 2 antibacterial yeast culture

[0041] (1) slant bacterial strain activation: inoculate frozen preserved Saccharomyces cerevisiae HKB-36 on the slant medium containing malt extract powder 145g / L, chloramphenicol 0.08g / L and agar 2%, let it stand at a constant temperature of 30°C, Cultivate for 48 hours to obtain activated yeast strains.

[0042] (2) Preparation of seed liquid: Inoculate the activated yeast strain with 1 loop into a liquid medium containing malt extract powder 145g / L, chloramphenicol 0.08g / L, and a pH value of 5.8-6.8. ℃-36℃ constant temperature shaker culture, rotation speed 120-200r / min; culture 18-30h.

[0043] (3) Liquid aerobic fermentation: liquid aerobic fermentation: inoculate the prepared seed liquid with 10%-20% inoculation amount into a new container containing malt extract powder 145g / L, chloramphenicol 0.08g / L, pH The expanded culture is carried out in a fermenter with a liquid medium whose value is 5.8-6.8, t...

Embodiment 3

[0046] The in vitro antibacterial experiment of embodiment 3 bacteriostatic yeast culture

[0047] Adopt the Oxford cup method to carry out antibacterial test, observe respectively the antibacterial effect of the yeast culture of bacteriostatic type yeast culture of the present invention and other 5 kinds of Saccharomyces cerevisiae to Escherichia coli, Staphylococcus aureus and Salmonella, and in addition to physiological Saline was used as a blank control, and ampicillin was added as a positive control.

[0048] Experimental results such as image 3 shown. image 3 Among them, d3 represents the bacteriostatic yeast culture of the present invention obtained from the fermentation of Saccharomyces cerevisiae HKB-36, and d3H, d5, d5H, d10 and d10H are respectively the yeast strains HKB-6, HKB-10, HKB-27, HKB-33 Common yeast cultures obtained by fermentation with HKB-46 respectively. The unmarked under the ampicillin in the middle is the normal saline blank control.

[0049] ...

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Abstract

The invention relates to piglet feed including bacteriostatic yeast culture, and a preparation method and applications thereof. The piglet feed includes, by weight, 50-70 parts of corns, 5-20 parts ofsoybean meal, 5-15 parts of extruded soybeans, 2-10 parts of whey powder, 2-4 parts of fish meal, 0-5 parts of plasma protein powder, 1-4 parts of soya-bean oil, 0.5-1.0 part of stone powder, 0.5-1.5parts of calcium hydrogen phosphate, 0.2-0.5 part of salt, 0.5-1.5 parts of premix, and 0.5-2.5 parts of bacteriostatic yeast culture. A preparation method and applications of the piglet feed are also included. The piglet feed has characteristics of inhibiting the growth of escherichia coli, staphylococcus aureus, salmonella and other harmful bacteria, so that the intestinal health of piglets canbe effectively improved, flora balance can be maintained, the immune functions of the piglets can be enhanced, diarrhoea can be prevented, the growth performance of the piglets can be enhanced, and the using of antibiotics in the feed can be reduced.

Description

technical field [0001] The invention relates to the field of feed industry, in particular to piglet feed containing antibacterial yeast culture and its preparation method and application. Background technique [0002] The growth performance of weaned piglets (including body weight gain or body weight at the end of nursery) is closely related to piglet health, subsequent growth and development, and even time to market. Due to changes in the environment, feed, management and other factors, weaned piglets are subject to greater stress, and are prone to symptoms such as diarrhea, poor appetite, and slow growth during production, which greatly affects the production efficiency of the pig industry. [0003] Diarrhea in weaned piglets is mainly due to the insufficiency of the intestinal mucosal barrier in piglets, which is vulnerable to the invasion of foreign pathogens during weaning, causing intestinal mucosal damage and diarrhea. Diarrhea is an urgent problem to be solved in pi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/30A23K50/60A23K10/16A23K10/12
CPCA23K50/30A23K50/60A23K10/16A23K10/12
Inventor 杨凤娟刘明张相飞欧淑琦
Owner 北京中农弘科生物技术有限公司
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