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Method for detecting allergens in cosmetics

A detection method and allergen technology, applied in the field of allergen detection in cosmetics, can solve the problems of insufficient anti-interference ability of complex matrix, false positive, cumbersome detection process, etc., to reduce the probability of false positive occurrence, high accuracy and sensitivity , The effect of simplifying the pre-processing steps

Active Publication Date: 2019-08-27
青岛元达生物科技有限公司
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Problems solved by technology

At present, the main detection methods for these allergen components include liquid chromatography, gas chromatography, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry, but there are some problems in the detection and analysis of traditional detection methods: (1) The anti-interference ability of the complex matrix is ​​insufficient, and false positives often occur; (2) Due to the different chemical properties of different types of allergens, the extraction method is often only established for a certain type of allergen, and there is no method for simultaneous extraction of several types of allergens (3) For different types of allergens, researchers often set different instrument parameters and establish different instrument detection methods, which cannot complete the screening of all components in one test, making the detection process too cumbersome

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  • Method for detecting allergens in cosmetics
  • Method for detecting allergens in cosmetics
  • Method for detecting allergens in cosmetics

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Experimental program
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Effect test

Embodiment 1

[0022] Instruments: obitrap high-resolution mass spectrometer (ThermoFisher, USA); CR22G II high-speed refrigerated centrifuge (Hitachi, Japan).

[0023] Reagents: cinnamyl alcohol, cinnamaldehyde, methylisothiazolinone, 2-amino-3-hydroxypyridine, 4-amino-2-hydroxytoluene, 2,4-diaminophenoxyethanol hydrochloride, N,N- Bis(2-hydroxyethyl)-p-phenylenediamine sulfate, 2-chloro-p-phenylenediamine sulfate, phenylmethylpyrazolone, 4-amino-3-nitrophenol, 4-nitro-phthalate Diamine, p-methylaminophenol sulfate, 6-hydroxyindole, N-phenyl-p-phenylenediamine, 2,7-naphthalenediol, 1,5-naphthalenediol were purchased from Shanghai Anpu, and diazoxane Urea was purchased from Acstandard in the United States, and the rest were purchased from Dr. Ehrenstorfer in Germany, with a purity of >95%. Toluene-2,5-diamine sulfate and 2-chloro-p-phenylenediamine sulfate are prepared with 2g / L ascorbic acid aqueous solution; 2-nitro-p-phenylenediamine, toluene-3,4- Diamine was prepared with methanol, and...

Embodiment 2

[0037] Screening Confirmation and Quantitative Analysis of Example 2 Actual Samples

[0038] According to the method determined in this paper, 8 kinds of commercially available hair dyes and 15 kinds of cosmetics were tested, and the detected fragrances, preservatives and dye components were consistent with the components indicated on the label, and their contents were all lower than those in my country's "Safety and Technical Specifications for Cosmetics" (2015 Edition)" limit requirements. 2-amino-3-hydroxypyridine and 2,4-diaminophenoxyethanol hydrochloride were detected in hair dye samples and methylisothiazolinone and methyl chloride were detected in body wash Taking isothiazolinone as an example, the screening and confirmation process of compounds in actual samples is introduced. First, carry out FullMS / ddMS2 detection on the sample, and compare the detection data with the retention time, accurate mass number of parent ion and secondary fragment ion in the database throu...

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Abstract

The invention provides a method for detecting allergens in cosmetics. The method comprises the steps of preparing a mixed standard solution of 56 allergen substances by a mixed solution of methanol and a 2g / L ascorbic acid aqueous solution, wherein single standard solutions are independently prepared for seven groups of isomers such as eugenol, isoeugenol and the like; carrying out mass spectrometry analysis in modes of positive-negative ion primary mass spectrometry full scanning and data-dependent secondary mass spectrometry scanning of a high-resolution mass spectrum of an electrostatic field orbitrap; collecting an accurate mass number of primary parent ions, retention time and secondary fragment ion information to establish a high-resolution mass spectrum database; carrying out mass spectrometry analysis on a to-be-detected sample by adopting extraction of the mixed solution of the methanol and the 2g 2g / L ascorbic acid aqueous solution; by utilizing the database, establishing screening and quantitative analysis methods by using Tracerfinder software; and detecting the allergen substances in the sample. The method is rapid, high in sensitivity and high in specificity.

Description

technical field [0001] The invention belongs to the technical field of compound analysis and detection, and in particular relates to a method for detecting allergens in cosmetics. Background technique [0002] Cosmetics are indispensable daily necessities in people's lives. However, due to the allergens such as spices, preservatives and dyes contained in cosmetics, as well as the diversity and specificity of the human immune system, consumers often cause skin and adnexal lesions due to the use of cosmetics, which is commonly referred to as Said cosmetic skin disease. According to the monitoring results of human adverse reactions to cosmetics in China, in recent years, with the increase in the types of cosmetics, the incidence of clinical cosmetic skin diseases has increased year by year. According to statistics, the vast majority of cosmetic allergies are caused by spices, preservatives and dyes. At present, the main detection methods for these allergen components include ...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 李兆杰鞠玲燕高飞徐成钢刘玉敏魏玮
Owner 青岛元达生物科技有限公司
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