Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of soluble extracellular polymeric substance of bacillus microorganism

An extracellular polymer and Bacillus technology, applied in the field of microorganisms, can solve the problems of non-biodegradation, high synthesis cost, non-regeneration, etc., and achieve the effects of easy cultivation, low cost, and preventing the formation of calcium scale

Pending Publication Date: 2019-08-27
YUNNAN UNIV
View PDF6 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these substances are often non-biodegradable and non-renewable, and most organic corrosion inhibitors are toxic, easy to cause environmental pollution and high synthesis cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of soluble extracellular polymeric substance of bacillus microorganism
  • Preparation method and application of soluble extracellular polymeric substance of bacillus microorganism
  • Preparation method and application of soluble extracellular polymeric substance of bacillus microorganism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: This embodiment is the preparation and application of the soluble extracellular polymer of Bacillus cereus

[0037] The preparation method of the soluble extracellular polymer of microorganisms of the genus Bacillus, the specific steps are as follows:

[0038] (1) Adjust the pH value of the Luria-Bertani liquid medium to 7.0, place it at a temperature of 121 ° C for 30 minutes after high-temperature sterilization as a Luria-Bertani liquid fermentation medium, and use a solid Luria-Bertani medium to treat Bacillus cereus After the bacillus was activated for 24 hours, it was inoculated into a Luria-Bertani liquid fermentation medium and cultivated for 36 hours to obtain a Bacillus microbial fermentation liquid;

[0039] (2) centrifuging the Bacillus microbial fermentation liquid in step (1) at a temperature of 4° C., leaving it to stand and taking the supernatant, which is dialyzed through a dialysis bag to obtain a soluble extracellular polymer solution; wh...

Embodiment 2

[0057] Embodiment 2: This embodiment is the preparation and application of the soluble extracellular polymer of Bacillus cereus

[0058] The preparation method of the soluble extracellular polymer of microorganisms of the genus Bacillus, the specific steps are as follows:

[0059] (1) Adjust the pH value of the Luria-Bertani liquid medium to 7.4, place it at a temperature of 110°C for 35 minutes after high-temperature sterilization as a Luria-Bertani liquid fermentation medium, and use a solid Luria-Bertani medium to treat Bacillus cereus After the bacillus was activated for 30 hours, it was inoculated into a Luria-Bertani liquid fermentation medium and cultivated for 28 hours to obtain a Bacillus microbial fermentation liquid;

[0060] (2) centrifuging the Bacillus microbial fermentation liquid in step (1) at a temperature of 5° C., leaving it to stand to take the supernatant, which is dialyzed through a dialysis bag to obtain a soluble extracellular polymer solution; wherein...

Embodiment 3

[0063] Embodiment 3: This embodiment is the preparation and application of the soluble extracellular polymer of Bacillus cereus

[0064] The preparation method of the soluble extracellular polymer of microorganisms of the genus Bacillus, the specific steps are as follows:

[0065] (1) Adjust the pH value of the Luria-Bertani liquid medium to 8.0, and place it at a temperature of 100°C for high-temperature sterilization for 40 minutes as the Luria-Bertani liquid fermentation medium, using a solid Luria-Bertani medium to treat spores of cereus After the bacillus was activated for 48 hours, it was inoculated into a Luria-Bertani liquid fermentation medium and cultivated for 24 hours to obtain a Bacillus microbial fermentation liquid;

[0066] (2) Centrifuge the Bacillus microbial fermentation liquid in step (1) at a temperature of 8° C., leave it to stand to take the supernatant, and dialyze the supernatant through a dialysis bag to obtain a soluble extracellular polymer solution...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method and application of a soluble extracellular polymeric substance of a bacillus microorganism, and belongs to the technical field of microorganisms. The preparation method comprises the following steps: adjusting the pH (Potential of hydrogen) value of a Luria-Bertani liquid culture medium to be 7.0-8.0, performing high temperature sterilization and then using the treated Luria-Bertani liquid culture medium as a Luria-Bertani liquid fermentation culture medium, adopting a solid Luria-Bertani culture medium, performing activation culture on bacillus for24-48 h, then inoculating the Luria-Bertani liquid fermentation culture medium with the bacillus subjected to the activation culture, and culturing for 24-36 h to obtain a bacillus microorganism fermentation liquor; placing the bacillus microorganism fermentation liquor under the condition of the temperature of 4-8 DEG C and performing centrifugal treatment, standing and taking supernatant liquor, and dialyzing the supernatant liquor through a dialysis bag to obtain a soluble extracellular polymeric substance solution; performing freeze drying on the soluble extracellular polymeric substancesolution to obtain soluble extracellular polymeric substance powder; dissolving the soluble extracellular polymeric substance powder in distilled water to prepare a soluble extracellular polymeric substance water solution with a preset concentration for standby use. The soluble extracellular polymeric substance provided by the invention is used as a dual-functional biological corrosion inhibitor and scale inhibitor.

Description

technical field [0001] The invention relates to a preparation method and application of a soluble extracellular polymer of microorganisms belonging to the genus Bacillus, belonging to the technical field of microorganisms. Background technique [0002] Scaling is one of the main problems plaguing the use of industrial cooling circulation equipment and seawater desalination equipment. This is also a major concern in some industrial processes, namely energy production. Dirt covering the metal surface will not only lead to under-deposit corrosion, but also greatly reduce its heat transfer efficiency, cause energy loss and waste, and even bring many safety accidents. In France, non-productive expenditures related to scale-up are estimated at 1.5 billion euros per year; the same costs are about $800 million in the UK, $3 billion in Japan, and $9 billion in the United States; Caused economic losses of more than 70 billion yuan, far exceeding the world average [4] ; In addition,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12P19/34C12P19/04C12P13/02C23F11/10C02F5/12C12R1/085C12R1/11C12R1/125C02F103/02C02F103/08
CPCC12P21/00C12P19/34C12P19/04C12P13/02C02F5/12C23F11/10C02F2303/08C02F2103/023C02F2103/08
Inventor 屈庆李顺灵李蕾
Owner YUNNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com