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A strain of Bacillus subtilis sl-3a, a tobacco stalk degrading bacterial agent and its preparation method and application

A technology of Bacillus subtilis and tobacco stalks, applied in the field of microorganisms, can solve the problems of bacterial pollution and inability to have strong cellulose degradation ability

Active Publication Date: 2020-10-20
FUYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing cellulose-degrading bacterial agents can only degrade the cellulose in the straw. After the composting is completed, the bacteria have not been completely killed. If the post-processing is not done properly, it will cause bacterial pollution. The ability to degrade cellulose can also release potassium and phosphorus in the soil in the later stage, which has a good application prospect
However, there is no report on the discovery of cellulose-degrading bacteria with comprehensive biological functions from tobacco stalk waste.

Method used

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  • A strain of Bacillus subtilis sl-3a, a tobacco stalk degrading bacterial agent and its preparation method and application
  • A strain of Bacillus subtilis sl-3a, a tobacco stalk degrading bacterial agent and its preparation method and application
  • A strain of Bacillus subtilis sl-3a, a tobacco stalk degrading bacterial agent and its preparation method and application

Examples

Experimental program
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Effect test

preparation example Construction

[0034] The present invention also provides a method for preparing the tobacco stalk-degrading bacterial agent described in the above technical solution, comprising the following steps: inoculating the Bacillus subtilis SL-3A in a sodium carboxymethylcellulose fermentation medium for 2-4 days of fermentation , to obtain the bacterial agent for degrading tobacco stalks. In the present invention, the sodium carboxymethylcellulose fermentation medium preferably uses peptone as the sole nitrogen source and sodium carboxymethylcellulose as the sole carbon source, and the C / N ratio of the sole carbon source to the sole nitrogen source is 2:1. In the present invention, the inoculum volume fraction of the Bacillus subtilis SL-3A is preferably 6-10%, more preferably 8%; the temperature of the fermentation culture is preferably 25-45°C, more preferably 37°C, pH The value is preferably 6.0-8.0, more preferably 7, and the fermentation culture further includes shaking, and the rotating spe...

Embodiment 1

[0038] Determination of the ability of SL-3A to degrade cellulose

[0039] 1.1 Experimental materials

[0040] 1.1 Test strains

[0041] The bacterial strain screened from tobacco stalk waste: SL-3A was used as the experimental material.

[0042] 1.2 Medium

[0043] (1) Common media for bacterial strain purification

[0044] LB medium, LB liquid medium;

[0045] (2) Degradation cellulase screening medium

[0046] Carboxymethylcellulose sodium medium (g / L): MgSO 4 ·7H 2 O 0.1, KH 2 PO 4 0.5, CaCl 2 0.1,K 2 HPO 4 2. Sodium carboxymethyl cellulose 10, (NH 4 ) 2 SO 4 2. Agar 18, pH 7.0-7.4;

[0047]Carboxymethyl cellulose enzyme activity (CMCase) detection medium (g / L): peptone 10, beef extract powder 10, sodium chloride 1.5, sodium carboxymethyl cellulose 10, KH 2 PO 4 1.0, MgSO 4 ·7H 2 O 0.3, pH 7.0;

[0048] Filter paper enzyme activity (FPase) detection medium (g / L): peptone 10, beef extract 10, sodium chloride 1.5, filter paper 0.05, KH 2 PO 4 1.0, ...

Embodiment 2

[0072] Study on the Growth-promoting Function of SL-3A Strain

[0073] In this example, the experiments of SL-3A dissolving phosphorus and potassium are studied.

[0074] 2.1 Materials

[0075] 2.1.1 Tested strains

[0076] Bacteria SL-3A was provided by the Microbiology Laboratory of Fuyang Normal University.

[0077] 2.1.2 Medium

[0078] (1) Common media for bacterial strain purification

[0079] LB medium, LB liquid medium;

[0080] (2) Determination medium for phosphorus solubilizing effect

[0081] Organophosphate solid medium (g / L): glucose 10g, (NH 4 ) 2 SO 4 0.5, NaCl 0.3, KCl 0.3, MgSO 4 ·7H 2 O 0.3, FeSO 4 ·7H 2 O 0.03, MnSO 4 4H 2 O 0.03, CaCO 3 5. Lecithin 2, agar 20, pH 7.0-7.5.

[0082] Organic phosphorus liquid medium (g / L): glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, CaCO 3 5g, lecithin 2g, pH 7.0-7.5. (During the experiment, replace lecithin with fresh egg...

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Abstract

The invention relates to a strain of Bacillus subtilis SL-3A and an application thereof, belonging to the technical field of microbes. The Bacillus subtilis SL‑3A of the present invention has a preservation number of GDMCC NO: 60629. The bacillus subtilis SL-3A of the present invention not only has a powerful function of degrading cellulose, but also has the function of decomposing phosphorus and potassium.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus subtilis SL-3A, a tobacco straw degrading bacterial agent, a preparation method and an application. Background technique [0002] Crop waste is large in quantity and widely distributed. If it is not properly disposed and utilized, it will not only be a waste of resources, but also cause serious pollution to the environment. Rational development and utilization of agricultural waste resources is conducive to the harmonious development of man and nature. It can not only reduce agricultural non-point source pollution, but also improve rural environmental conditions and improve the quality of life of farmers. [0003] With the enhancement of people's awareness of environmental protection and the country's vigorous advocacy of environmental protection, people gradually reduce the use of chemical fertilizers in agricultural production, begin to explore the development...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/00C05F11/08C05F17/20C12R1/125
CPCC05F11/00C05F11/08C05F17/00C12N1/205C12R2001/125Y02W30/40
Inventor 梅金飞盛良全吴福芳徐华杰刚利萍余梅霞戴亚
Owner FUYANG NORMAL UNIVERSITY
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