A kind of bio-organic fertilizer fermentation agent
A technology of bio-organic fertilizer and fermentation inoculum, applied in the field of bio-organic fertilizer fermentation inoculum, can solve problems such as inability to synchronously ferment poultry manure and poultry feathers, inability to complete the fermentation and transformation of poultry feathers, and underutilization of poultry feathers, etc. Pathogenic microorganisms, less total nutrient loss of compost, and the effect of solving production difficulties
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Embodiment 1
[0036] The bio-organic fertilizer fermenting agent of the present invention, its active component comprises following bacterial species: preservation number is Bacillus amyloliquefaciens K1 of CICC.NO.10888, preservation number is Bacillus subtilis D1 of preservation number CICC.NO.10073, preservation number is Bacillus subtilis D2 with CICC.NO.10090, Bacillus licheniformis S1 with preservation number CICC.NO.10037, Bacillus licheniformis S2 with preservation number CICC.NO.10092, thermophile with preservation number ACCC.NO.30346 Sporotrichum T1.
[0037] The preparation method of seed bacterial agent of the present invention is:
[0038] 1) Inoculate Bacillus amyloliquefaciens K1 in medium I with a pH of 7.0 prepared with 3 g of peptone, 2 g of beef extract, 3 g of NaCl, 10 g of agar, and 0.8 L of distilled water, and culture on a shaking table at 35°C 1 day, the shaking speed was 100rpm, and the Bacillus amyloliquefaciens seed agent N1 was obtained;
[0039] 2) Inoculate ...
Embodiment 2
[0048] The bio-organic fertilizer fermenting agent of the present invention, its active component comprises following bacterial species: preservation number is Bacillus amyloliquefaciens K1 of CICC.NO.10888, preservation number is Bacillus subtilis D1 of preservation number CICC.NO.10073, preservation number is Bacillus subtilis D2 with CICC.NO.10090, Bacillus licheniformis S1 with preservation number CICC.NO.10037, Bacillus licheniformis S2 with preservation number CICC.NO.10092, thermophile with preservation number ACCC.NO.30346 Sporotrichum T1.
[0049] The preparation method of seed bacterial agent of the present invention is:
[0050] 1) Inoculate Bacillus amyloliquefaciens K1 in medium I with a pH of 7.0 prepared with 4 g of peptone, 3 g of beef extract, 4 g of NaCl, 12 g of agar, and 0.9 L of distilled water, and culture on a shaking table at 36°C For 2 days, the shaking speed was 120rpm, and Bacillus amyloliquefaciens seed agent N1 was obtained;
[0051] 2) Inoculate...
Embodiment 3
[0061] The bio-organic fertilizer fermenting agent of the present invention, its active component comprises following bacterial species: preservation number is Bacillus amyloliquefaciens K1 of CICC.NO.10888, preservation number is Bacillus subtilis D1 of preservation number CICC.NO.10073, preservation number is Bacillus subtilis D2 with CICC.NO.10090, Bacillus licheniformis S1 with preservation number CICC.NO.10037, Bacillus licheniformis S2 with preservation number CICC.NO.10092, thermophile with preservation number ACCC.NO.30346 Sporotrichum T1.
[0062] The preparation method of seed bacterial agent of the present invention is:
[0063] 1) Inoculate Bacillus amyloliquefaciens K1 in medium I with a pH of 7.0 prepared with 5 g of peptone, 4 g of beef extract, 5 g of NaCl, 14 g of agar, and 1 L of distilled water, and culture it on a shaking table at 37°C for 3 days, the shaking speed was 140rpm, and the Bacillus amyloliquefaciens seed agent N1 was obtained;
[0064] 2) Inoc...
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