Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Inducing medium and method for inducing fibroblasts to secrete antimicrobial peptide, fibroblasts and application

A technology of fibroblasts and induction medium, applied in the field of cell engineering, can solve the problems of inability to cure, poor effect, and inability of fibroblasts to secrete antimicrobial peptides, so as to improve the skin condition, improve the ability, and improve various skin problems. Effect

Inactive Publication Date: 2019-08-23
西安飞如生物科技有限公司
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibiotics and hormonal drugs have the disadvantages of drug resistance, strong side effects, and large individual differences.
These two types of treatment methods all have the disadvantages of long treatment cycle, short validity period, and inability to cure the disease. Especially when antibiotics are used for treatment, with the increase in the use of antibiotics, the emergence of various drug-resistant bacteria makes the effect of antibiotic treatment more and more effective. Difference
The antimicrobial peptide itself is a biological macromolecule. If it is applied externally, it cannot be absorbed through the skin. Oral administration will lead to the degradation of the antimicrobial peptide.
Directly transplanted dermal fibroblasts cannot secrete antimicrobial peptides and have no effect on alleviating inflammatory skin problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Inducing medium and method for inducing fibroblasts to secrete antimicrobial peptide, fibroblasts and application
  • Inducing medium and method for inducing fibroblasts to secrete antimicrobial peptide, fibroblasts and application
  • Inducing medium and method for inducing fibroblasts to secrete antimicrobial peptide, fibroblasts and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] This embodiment provides an induction medium for inducing skin fibroblasts to produce antimicrobial peptides. According to volume percentage, the induction medium contains 95% high-sugar DMEM / F12 and 5% HPL; the induction medium also contains SB431542 at a final concentration of 2.5 μmol / L.

Embodiment 2

[0088] This embodiment provides an induction medium for inducing skin fibroblasts to produce antimicrobial peptides. According to volume percentage, the induction medium contains 90% high-sugar DMEM / F12 and 10% HPL; the induction medium also contains SB431542 at a final concentration of 2.5 μmol / L.

Embodiment 3

[0090] This embodiment provides an induction medium for inducing skin fibroblasts to produce antimicrobial peptides. According to volume percentage, the induction medium contains 85% high-sugar DMEM / F12 and 15% HPL; the induction medium also contains SB431542 at a final concentration of 2.5 μmol / L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an inducing medium and method for inducing fibroblasts to secrete antimicrobial peptide, the fibroblasts and application, and relates to the field of cell engineering. The inducing medium contains a basic medium, platelet lysate and a TGF-beta signal channel inhibitor. The inducing medium can activate the adipose differentiation proficiency of the skin fibroblasts and secrete the antimicrobial peptide in the adipose differentiation process. When the inducing medium is used for inducing the fibroblasts to secrete the antimicrobial peptide, operation is easy, the controllability is high, and the problem of lacking the fibroblasts capable of secreting the antimicrobial peptide in vitro in the prior art is solved.

Description

technical field [0001] The invention relates to the field of cell engineering, in particular to an induction medium and method for inducing fibroblasts to secrete antimicrobial peptides, fibroblasts and applications. Background technique [0002] Human skin fibroblasts, located in the dermis of the skin, are a kind of cells with certain differentiation potential originating from the mesenchyme. At present, it is believed that skin fibroblasts have the function of secreting collagen, elastic fibers and other extracellular matrix, so they have a wide range of applications in the fields of medical plastic surgery and skin damage repair. [0003] The latest research shows that skin fibroblasts have the potential to differentiate into fat in the dermis of the skin, and can secrete antimicrobial peptides during the differentiation process, which is crucial to the innate immune function of the skin. Antimicrobial peptide is a new type of antibacterial protein, which has many advan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12P21/00A61K35/33A61K38/02A61P17/00A61P17/10A61P31/04A61K8/99A61K8/64A61Q19/00A61Q19/08
CPCA61K8/64A61K8/99A61K35/33A61K38/02A61P17/00A61P17/10A61P31/04A61Q19/00A61Q19/08C12N5/0656C12N2500/84C12N2501/15C12P21/00
Inventor 李相其
Owner 西安飞如生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com