A kind of extraction method of trilobatin

Abstract

The invention discloses a method for extracting trilobatin, which comprises the following steps: step 1, taking leaves of Cordyceps sinensis, pulverizing, adding water for heating extraction, heating and extracting and filtering while hot to obtain filtrate; step 2, freezing and refrigerating the filtrate; Step 3, take out the frozen filtrate, then thaw at a temperature below 40°C, and filter to obtain a precipitate; Step 4, dry the precipitate; Step 5, put the dried precipitate into a heated ethanol solution to dissolve, and the temperature of the ethanol solution is 60 °C. -80°C; then the ethanol solution is cooled to -20-40°C to obtain crystals; in step 6, the crystals are dried to obtain pure yellow trilobatin. The invention only needs to be filtered after freezing and redissolving, and then heat-saturated and crystallized to obtain high-purity trilobatin, which is short in time, simple in operation, easy in industrialized production, more environmentally friendly, and less harmful to the operator's body.

Description

technical field [0001] The invention belongs to the field of medicine, and particularly relates to a method for extracting trilobatin. Background technique [0002] Lithocarpus litseifolius (Hance) Chun is an evergreen plant of the Fagaceae family. It is mostly distributed in the low mountains and dense clumps in the south of the Yangtze River. It has medicinal and edible effects and can be used as tea, sugar and medicine plant i. Traditional folk experience shows that Mu Jiang Ye Ke has the functions of clearing heat and diuresis, nourishing the liver and nourishing the stomach, resolving phlegm, expelling wind and lowering blood pressure. Modern medical research has proved that Alpinia officinalis has anti-oxidative, immunosuppressive, memory-improving, plant growth and development, hypoglycemic and other pharmacological effects. Among them, trilobulin is one of the main active components of Cordyceps sinensis, which has anti-diabetic, hypoglycemic, anti-HIV-1 infection,...

AI Technical Summary

Problems solved by technology

At present, there are many domestic researches on phlorizin in Alpinia officinalis, but less research on trilobatin

The medicinal health mechanism of trelobatin has not been fully developed and utilized

Most of the patents use ethanol aqueous solution for extraction and generally use chromatography for further separation, which takes a long time; or uses high-speed countercurrent chromatography, which requires high testing and extraction costs

For example, the steps of separating trilobatin monomer by Zhang Yazhou and others are cumbersome and time-consuming

In addition, traditional recrystallization mostly uses methanol solvent, and methanol is very toxic

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