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Neutrophil gelatinase-associated lipocalin detection kit

A lipocalin and neutrophil technology, applied in biological testing, measuring devices, material testing products, etc., can solve the problems of reducing the stability of the detection kit, limiting the linear detection range, restricting the promotion of clinical applications, etc. The effect of improving detection sensitivity and improving stability

Inactive Publication Date: 2019-08-16
芜湖森爱驰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the immune activity of the antibody in the conventional NGAL immunoturbidimetric assay kit gradually decays with the storage time, which reduces the stability of the kit, limits the linear detection range, and restricts the promotion of clinical applications.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0027] A neutrophil gelatinase-associated lipocalin detection kit, comprising independent reagents R1 and R2, the reagent R1 is composed of the following substances: phosphate buffer 100mmol / L; glutathione 1mmol / L L; TritonX-1000.8g / L; sodium azide 0.5g / L; bovine serum albumin 22g / L; guanidine thiocyanate 0.5g / L, and the pH value of the reagent R1 is 6.5-7.5;

[0028] Described reagent R2 is made up of following material: phosphate buffer saline 135mmol / L; Dextran sulfate sodium 10g / L; Blocking agent 3mg / L (the composition of described blocking agent is as follows: fish skin gelatin protein 2wt%, two Ethylenetriaminepentaacetic acid 3wt%, NH2-PEG 2.5wt% and chitosan 3.5wt%, phosphate buffer saline 89wt%, the concentration of phosphate buffer saline is 120mmol / L); Sucrose 8mg / L; Neutral The nanosphere 45mg / L (diameter is 250nm) of granulocyte gelatinase-associated lipocalin antibody; Sodium azide 0.03g / L; Wherein, the component concentration in the reagent R2 is the final conce...

Embodiment 2

[0031] A detection kit for neutrophil gelatinase-associated lipocalin, comprising independent reagents R1 and R2, wherein the reagent R1 consists of the following substances: phosphate buffer 100mmol / L; glutathione 1.2mmol / L; Triton X-1000.5g / L; p-hydroxybenzoic acid 0.8g / L; bovine serum albumin 25g / L; guanidine isothiocyanate 0.8g / L, and the pH value of the reagent R1 is 6.5-7.5;

[0032] Described reagent R2 is made up of following material: phosphate buffer saline 140mmol / L; PEG-8000 12g / L; Blocking agent 5mg / L (the composition of described blocking agent is as follows: fish skin gelatin protein 2.5wt%, two sub- Ethyltriaminepentaacetic acid 3.5wt%, NH2-PEG 3wt% and chitosan 4wt%, phosphate buffer saline 87wt%, the concentration of phosphate buffer saline is 120mmol / L); Sucrose 6mg / L; Neutrophil The nanosphere 50mg / L (diameter is 200nm) of gelatinase-associated lipocalin antibody; p-hydroxybenzoic acid 0.04g / L; Wherein, component concentration is the final concentration of...

Embodiment 3

[0035] A neutrophil gelatinase-associated lipocalin detection kit, comprising independent reagents R1 and R2, the reagent R1 is composed of the following substances: 90mmol / L of phosphate buffer; 0.8mmol of glutathione / L; Tween-201g / L; ethyl p-hydroxybenzoate 0.2g / L; bovine serum albumin 20g / L; guanidine thiocyanate 0.2g / L, and the pH value of the reagent R1 is 6.5-7.5;

[0036] Described reagent R2 is made up of following material: phosphate buffer saline 120mmol / L; PEG-4000 10g / L; Blocking agent 6mg / L (the composition of described blocking agent is as follows: fish skin gelatin protein 1.5wt%, disubstrate Ethyltriaminepentaacetic acid 4wt%, NH2-PEG 4wt% and chitosan 2.5wt%, phosphate buffer saline 88wt%, the concentration of phosphate buffer saline is 120mmol / L); Sucrose 10mg / L; Neutrophil The nanosphere 36mg / L (diameter is 200nm) of gelatinase-associated lipocalin antibody; Ethyl p-hydroxybenzoate 0.02g / L; Wherein, the component concentration in the reagent R2 is the final...

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Abstract

The present invention relates to the field of biological detection technology, and in particular to a neutrophil gelatinase-associated lipocalin detection kit, the neutrophil gelatinase-associated lipocalin detection kit comprises mutually independent reagent R1 and reagent R2, and the detection kit is characterized in that: the reagent R1 comprises the following substances: 80-120 mmol / L of phosphate buffer solutions; 0.5-1.5mmol / L of glutathione; 0.02-1.5g / L of surfactant; 0.05-2g / L of preservatives; 15-30g / L of bovine serum albumin; 0.02-1g / L of dissociation agents, and the reagent R1 hasa pH of 6.5 to 7.5; and the reagent R2 comprises the following materials: 100-150 mmol / L of phosphate buffer solutions; 8-15 g / L of coagulant; 2-8mg / L of blocking agents; 5-15mg / L of sucrose; 20-50mg / L of nano-microspheres of neutrophil gelatinase-associated lipocalin antibodies; 0.01-0.05g / L of preservatives; wherein component concentration in the reagent R2 is final concentration of the component in the reagent, and pH of the reagent R1 is 6.5 to 7.5. The neutrophil gelatinase-associated lipocalin detection kit has high detection sensitivity.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a neutrophil gelatinase-associated lipocalin detection kit. Background technique [0002] Neutrophil gelatinase-associated lipocalin (NGAL) is a growth factor in physiological state, mainly involved in the occurrence and growth of early renal epithelium. The expression level is low in normal tissues including kidney, lung, stomach and colon epithelial tissues. In the process of ischemic and toxic kidney injury, NGAL in the renal tubular epithelial cells will increase significantly, and within the first two hours, the level of NGAL in urine and blood will increase significantly, so NGAL is the early stage of acute kidney injury. It is a sensitive marker, and its expression level is related to renal ischemia time; NGAL is also a potential new marker to reflect chronic kidney damage. In addition, NGAL levels were significantly higher in the initial and relapse phases ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893
Inventor 王晓波朱雪姣朱海峰
Owner 芜湖森爱驰生物科技有限公司
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