Clostridium welchii and application thereof

A technology of bacillus and strains, applied in the field of microorganisms, can solve the problems of soil without governance value, soil pollution, and threats to higher organisms

Inactive Publication Date: 2019-08-09
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heavy metals cannot be biodegraded, but they are bioaccumulative and can directly threaten higher organisms including humans. Relevant experts pointed out that heavy metal pollution to soil is irreversible, and the contaminated soil has no treatment value, and can only be avoided by adjusting planting varieties

Method used

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  • Clostridium welchii and application thereof
  • Clostridium welchii and application thereof
  • Clostridium welchii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Screening and identification of a strain of Bacillus wischii

[0015] 1. Domestication of Eichhornia crassipes

[0016] The water hyacinth used in the experiment was collected from Nanhu Lake, Hongshan District, Wuhan. Rinse the collected water hyacinth with tap water and put it in tap water for pre-cultivation for one week. Select water hyacinths of the same size and growth and rinse them with deionized water. Then put Eichhornia crassipes in Hoagland nutrient solution containing 2.0mg / L Cd ion for 4 weeks. Obtain domesticated water hyacinth plants.

[0017] 2. Isolation of a strain of Bacillus wechneri

[0018] Obtain the domesticated plants and select the roots with high enrichment for strain screening: Wash the roots of Eichhornia crassipes stored in the refrigerator at -80℃ with deionized water three times, then perform surface disinfection, and immerse the intact plant tissues in 75 % Ethanol solution for 2 min, then soak in 3% sodium hypochlorite solution f...

Embodiment 2

[0022] Example 2 The maximum tolerance concentration of strain LX5 to heavy metals

[0023] The solid medium containing heavy metal ions was prepared separately, with a concentration starting from 0.1 mM / L and increasing by 0.5 mM. Streak and inoculate on fresh LB solid medium, culture for 48h at 30℃ for activation, pick a single colony and inoculate it into LB liquid medium, culture with shaking at 30℃, 120r / min for 24h, collect bacteria with OD600 of 1.5 Liquid press 100, 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 Proportional dilution, respectively dipping in each concentration of bacterial liquid spot inoculation on the heavy metal solid medium. Cultivate at 30°C and observe and record the growth of bacteria every 24h. The minimum heavy metal concentration that inhibits its obvious growth within 120h, that is, the maximum tolerable concentration of the bacteria, is shown in Table 1.

[0024] Table 1-Statistical Table of Tolerance Concentration of Strains of the Invention

[0025] ...

Embodiment 3

[0026] Example 3 Strain LX5 improves the concentration of heavy metals in Eichhornia crassipes

[0027] Eichhornia crassipes was cultured for 15 days in Hoagland nutrient solution containing 2.0mg / L Cd ion, and the strain LX5 was added after the enrichment ability of foreign bacteria-side heavy metals was not added.

[0028] As a result, compared with the control group, the treatment group with the exogenous added strain LX5 increased the Cd content in the roots from 0.498mg / L to 0.729mg / L, an increase of 46.3%; the stems and leaves increased from 0.090mg / L to 0.149mg / L, an increase of 64.4%.

[0029] Table 2

[0030] Cd(mg / L) Root Stem and leaf test group0.7290.149 Control group 0.4980.09

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Abstract

The invention provides clostridium welchii. The preservation number of the clostridium welchii is CCTCC NO.M2019204. A microorganism is screened from a domesticated water hyacinth root by an inventor,it is found that the strain has very strong resistance to heavy metals, and the strain is identified as the clostridium welchii by colony morphology, biochemistry and 16S rDNA sequencing analysis. The clostridium welchii can improve the enrichment capacity of water hyacinths to heavy metals including at least one of Cd, Zn, Mn, Ni, Cu and Pb. Meanwhile, IAA and ACC deaminase can be released to promote the growth of the water hyacinths.

Description

Technical field [0001] The present invention relates to the field of microorganisms, in particular to a strain of Bacillus welch and its application. Background technique [0002] The original meaning of heavy metals refers to metals with a specific gravity greater than 5 (metals with a density greater than 4.5 grams per cubic centimeter), including gold, silver, copper, iron, lead, etc. Heavy metal pollution is different from the pollution of other organic compounds. Many organic compounds can reduce or eliminate their harmfulness through physical, chemical or biological purification of nature itself. The heavy metals are enriched and difficult to degrade in the environment. At present, due to the mining, smelting, and processing of heavy metals in China, many heavy metals such as lead, mercury, cadmium, and cobalt have entered the atmosphere, water, and soil, causing serious environmental pollution. For example, heavy metals discharged with wastewater can accumulate in algae...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A01N63/00A01P21/00C12R1/07C02F101/20
CPCA01N63/00C02F3/34C02F2101/20C12N1/205C12R2001/07
Inventor 万小琼徐腾起李赟泽朱端卫赵建伟周文兵华玉妹刘广龙胡金龙肖乃东
Owner HUAZHONG AGRI UNIV
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