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Combined extraction method of purslane polysaccharide and total flavone on basis of micellar medium treatment

A polysaccharide extraction and purslane technology, applied in the field of joint extraction of purslane high-purity polysaccharides and total flavonoids extracts, can solve the problem of extraction of high-purity purslane polysaccharides and flavonoids, waste of active substances, difficult separation, etc. question

Active Publication Date: 2019-08-09
NANJING XIAOZHUANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Although the prior art reports on the extraction of polysaccharides and flavonoids from purslane, as the two main active components of purslane, there has been no simultaneous extraction of high-purity purslane in large quantities from the whole herb of purslane. Polysaccharides and flavonoids of purslane were reported, while the extraction of a single component resulted in the waste of the remaining active substances
Therefore, on the basis of the prior art, it is necessary to provide a combined process method for the separation and purification of purslane polysaccharides and total flavonoids that is efficient, concise, and easy to realize industrial production, so as to solve the problem of extracting polysaccharides and flavonoids from purslane. Existing waste of components, difficult separation of polysaccharides and flavonoids, and low extraction purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] (1) Take 1 kg of the clean purslane herb after dedusting and drying, dry it at 55°C for 3 hours, and pass through a 60-mesh sieve to obtain purslane powder, wrap it in filter paper, wrap it with gauze for external use, and use 90 %volume ethanol 10L immersion for 6h, Soxhlet extraction in a Soxhlet extractor at 55°C for 3h.

[0088] (2) After the Soxhlet extraction is completed, take out the filter bag, dry the powder to obtain degreased purslane medicinal powder; filter the extract with a microfiltration membrane, distill the filtrate under reduced pressure to recover the ethanol solvent, and obtain the initial extraction of total flavonoids The concentrated solution of the substance is about 510ml; the concentrated solution is diluted to 1L with 75% ethanol, shaken and extracted with 400ml petroleum ether for 3 times, each time for 15min, and the fat-soluble pigments such as chlorophyll and carotene are removed at the same time to obtain the degreased of the extract. ...

Embodiment 2

[0102] (1) Take 5 kg of clean purslane herb after dedusting and drying, grind it through a 60-mesh sieve, wrap it in filter paper, wrap it with gauze for external use, soak it for 6 hours with 50 L of ethanol with 95% volume, and put it in a Soxhlet extractor. Soxhlet extraction was carried out at 60°C for 4 hours, and the raw materials were extracted in three batches;

[0103] (2) After the Soxhlet extraction is completed, take out the filter bag to obtain the degreased purslane medicinal material powder, dry the powder for subsequent use; filter the extract with a microfiltration membrane, and distill the filtrate under reduced pressure to reclaim the ethanol solvent to obtain the total flavonoids The concentrated solution of the primary extract is about 2L; the concentrated solution is diluted to 3.5L with 75% ethanol, and extracted with 2L petroleum ether for 3 times for degreasing treatment, each time for 15min, to obtain the degreased extract.

[0104] (3) The degreased ...

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PUM

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Abstract

The invention provides a high-efficiency combined extraction method of high-purity purslane polysaccharide and total flavone, and relates to a method for deproteinizing and decoloring an extracting solution. The high-efficiency combined extraction method can achieve efficient and rapid extraction of purslane polysaccharide and total flavone from portulaca oleracea L. in a large scale, and single-step operation of specific micellar medium extraction is adopted for jointly removing protein and pigment components in a polysaccharide extraction step, and correspondingly the defect is avoided thatprotein removal and pigment removal need to be independently carried out during purslane polysaccharide purification in the prior art; the extraction flow is shortened, and the method is suitable forlarge-scale extraction.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a preparation method for jointly extracting high-purity polysaccharides and total flavonoids from purslane. Background technique [0002] Purslane (Portulaca oleracea L.) is an annual succulent herb belonging to the family Portulaca oleracea. It is widely distributed and has a large yield. It is a common Chinese herbal medicine and wild vegetable. It is one of the wild plants designated by the Ministry of Health as medicinal and edible. As a traditional Chinese medicine, purslane has the effects of clearing away heat and detoxification, cooling blood and reducing swelling. Modern medical research shows that it also has the effects of anti-atherosclerosis, anti-bacterial, anti-inflammatory, and hypoglycemic effects. [0003] The main active substances rich in purslane are polysaccharides and flavonoids, in addition, it also contains a large amount of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P31/04A61P31/10A61P29/00C08B37/00
CPCA61K36/185A61K2236/333A61K2236/51A61K2236/55A61P29/00A61P31/04A61P31/10C08B37/0003
Inventor 张边江唐宁陈全战
Owner NANJING XIAOZHUANG UNIV
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