Method of isolating botulinum toxin from botulinum toxin-containing solution

Abstract

Provided is a method of isolating a botulinum toxin type A macro complex from a botulinum toxin-containing solution, the method including performing anion exchange chromatography and cation exchange chromatography.

Description

technical field [0001] Cross References to Related Applications [0002] This application claims the benefit of Korean Patent Application No. 10-2016-0127540 filed with the Korean Intellectual Property Office on October 4, 2016, the disclosure of which is incorporated herein by reference in its entirety. [0003] The present disclosure relates to methods of isolating botulinum toxin from a botulinum toxin-containing solution. Background technique [0004] From 1890 to the present, Clostridium strains that secrete neurotoxic toxins have been discovered, and the properties of the toxins secreted by these strains have been studied in the past 70 years (Schant, E.J. et al., Microbiol. Rev. 56 ; 80; 1992). [0005] The genus Clostridium includes more than 127 species, divided according to their morphology and function. Clostridium botulinum is an anaerobic and Gram-positive bacterium that produces powerful polypeptide neurotoxins that cause a neuroparalytic disease, botulism, ...

AI Technical Summary

Problems solved by technology

[0011] According to this method, there is a problem that during the purification of the botulinum toxin protein, the neurotoxic component (i.e. a neurotoxic molecule of about 150 kDa), the medium complex (300 kDa) or the large complex (500 kDa) is dissociated and separated from the botulinum toxin bacillus toxin protein removed

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