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Method for detecting piperine in Chinese patent medicine for weight loss and health food

A technology of health food and detection method, which is applied in the field of medical detection, and can solve the problems of undetermined dosage, no screening and quantitative methods, etc.

Inactive Publication Date: 2019-08-02
苏州市药品检验检测研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The piperine monomer compound has not been approved by the state and can be used as a weight loss health food additive. Quantitative method, the present invention establishes a rapid test method for piperine, and conducts methodological verification for qualitative screening, confirmation and content determination of this substance

Method used

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  • Method for detecting piperine in Chinese patent medicine for weight loss and health food
  • Method for detecting piperine in Chinese patent medicine for weight loss and health food
  • Method for detecting piperine in Chinese patent medicine for weight loss and health food

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Embodiment 1

[0082] A method for detecting piperine in weight-loss Chinese patent medicines and health foods, comprising the following steps:

[0083] (1) Chromatographic conditions:

[0084] Chromatographic column: ACQUITY UPLC®HSS T3, 100mm×2.1mm, 1.8μm; detector: diode array detector; detection wavelength: 340nm; scanning wavelength range 190-400nm; mobile phase: A-0.1% formic acid solution, B- 0.1 % formic acid-acetonitrile; flow rate: 0.3mL / min; column temperature: 30℃; injection volume: 1μL; elution gradient program: 0-2min, 10%B; 7-9min, 90%B; %B;

[0085] Mass Spectrometry Conditions:

[0086] Electrospray positive ion mode; Capillary voltage: 3500V; Drying gas flow rate: 10.0L / min; Atomizing gas pressure: 40psig; Drying gas temperature: 350°C; Fragmentation voltage: 135V; Mass spectrometry; acquisition mode: targetMS; parent ion: 286.1453, retention time: 6.04min (chromatogram), 6.15min (mass spectrogram); collision energy: 15V, 30V; mass spectrometry scanning range: 50-1700; ...

Embodiment 2

[0096] A method for detecting piperine in weight-loss Chinese patent medicines and health foods, comprising the following steps:

[0097] (1) Chromatographic conditions:

[0098] Chromatographic column: ACQUITY UPLC®HSS T3, 100mm×2.1mm, 1.8μm; detector: diode array detector; detection wavelength: 340nm; scanning wavelength range 190-400nm; mobile phase: A-0.1% formic acid solution, B-0.1 % formic acid-acetonitrile; flow rate: 0.3mL / min; column temperature: 30℃; injection volume: 1μL; elution gradient program: 0-2min, 10%B; 7-9min, 90%B; %B;

[0099] Mass Spectrometry Conditions:

[0100] Electrospray positive ion mode; Capillary voltage: 3500V; Drying gas flow rate: 10.0L / min; Atomizing gas pressure: 40psig; Drying gas temperature: 350°C; Fragmentation voltage: 135V; Mass spectrometry; acquisition mode: targetMS; parent ion: 286.1453, retention time: 6.04min (chromatogram), 6.15min (mass spectrogram), collision energy: 15V, 30V; mass spectrometry scanning range: 50-1700;

...

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Abstract

The invention provides a method for detecting piperine in Chinese patent medicine for weight loss and health food. An ultra-high performance liquid chromatography-ultraviolet spectrum-time of flight mass spectrometry is adopted to carry out qualitative screening and content determination. Chromatographic conditions comprise that a chromatographic column is a C18 chromatographic column; a detectoris a diode array detector; a detection wavelength is 340nm; a scanning wavelength range is 190-400nm; a mobile phase includes A-0.1% formic acid solution and B-0.1% formic acid-acetonitrile; a flow rate is 0.3-0.4mL / min; a column temperature is 25-40 DEG C; a sample injection amount is 0.1-2muL; and an elution gradient program includes 10% B for 0-2min; 90% B for 7-9min;and 10% B for 9.1-10min. Byusing the method, exclusive, sensitive and rapid primary screening can be performed, and the piperine added in the Chinese patent medicine for weight loss and the health food is confirmed. The methodis mainly suitable for analysis of the piperine in tablets and capsule dosage forms.

Description

technical field [0001] The invention belongs to the field of medical detection and relates to a detection method of chemicals, in particular to a detection method of piperine in weight-loss Chinese patent medicines and health food. Background technique [0002] Piperine (CAS number: 94-62-2, molecular formula: C 17 h 19 NO 3 , molecular weight: 285.1), is a kind of alkaloid, which is the main component of the source of black pepper spiciness. It is used in some forms of traditional medicine and pesticides. Pharmacological studies have found that it has anti-oxidation, immune regulation, anti-tumor, and promotion of drug metabolism. In recent years, a Korean study confirmed that piperine has the effect of preventing fat accumulation in the body. [0003] The piperine monomer compound has not been approved by the state and can be used as a weight loss health food additive. Quantitative method, the present invention establishes a rapid test method for piperine, and conduc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 陈卫张斌郑佳新郑梅张珊珊陈丽波赵琪贾昌平钱叶飞
Owner 苏州市药品检验检测研究中心
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