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Aptamer-nickel-iron cyanide nanoparticle-RGO electrode preparation method and application

A nucleic acid aptamer and nanoparticle technology, applied in the field of electrochemically modified electrodes, can solve the problems of high cost, complicated operation and time-consuming, and achieve the effects of convenient operation, high sensitivity and simple method

Active Publication Date: 2019-08-02
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a nucleic acid aptamer-nickel ferricyanide nanoparticle-RGO electrode and its preparation method, which is used for rapid, highly sensitive and highly selective detection of PCB77, so as to overcome the existing detection method of PCB77 Complicated operation, time-consuming and high cost

Method used

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  • Aptamer-nickel-iron cyanide nanoparticle-RGO electrode preparation method and application
  • Aptamer-nickel-iron cyanide nanoparticle-RGO electrode preparation method and application
  • Aptamer-nickel-iron cyanide nanoparticle-RGO electrode preparation method and application

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Embodiment 1

[0031] This embodiment provides a preparation of an electrochemically modified electrode for detecting PCB77.

[0032] The preparation method of PDDA-protected RGO is: measure 3.0mL of 1.2mg / mL graphene oxide solution in a reaction bottle, add 55.0ul of 10.5wt% PDDA and mix well. Then, adjust the pH to 10 by 0.13mL 28% ammonia water . Add 50.0 uL of 80% hydrazine hydrate, stir vigorously for 20 min, and then set the volume to 10 mL. Put the reaction bottle in a 60° C. water bath and heat for 3.5 h to obtain a stable black suspension RGO.

[0033] The synthesis method of nickel-iron nanoparticles-RGO composite material is as follows: use 0.1M HCl to adjust the pH of the RGO solution to 3, and adjust the concentration to 20.0mM K 3 [Fe(CN) 6 ] and Ni(NO 3 )2 Under rapid stirring, add to the RGO solution, first Fe(CN) 6 3- Due to electrostatic interaction, it is adsorbed on the surface of RGO, and then the same amount of Ni(NO 3 ) 2 After working with the solution, continu...

Embodiment 2

[0036] The present embodiment provides an electrochemical analysis method for detecting PCB77, and the analysis steps are as follows:

[0037] (1)) Prepare a series of PCB77 standard solutions with different concentrations, ranging from 1.0 to 300.0 ng / L;

[0038] (2) With the nucleic acid aptamer-nickel ferricyanide nanoparticles-RGO electrode as the working electrode, the platinum electrode as the counter electrode, and the silver-silver chloride electrode as the reference electrode, the three electrodes were placed in 0.1M phosphate buffer (pH 7.41) as the electrolyte solution, the standard solution prepared in step (1) was sequentially reacted with the nucleic acid aptamer-nickel ferricyanide nanoparticles-RGO electrode, the reaction time was 60min, and the different concentrations were recorded by differential pulse voltammetry The peak current corresponding to PCB77, the differential pulse voltammogram of the peak current changing with the concentration of PCB77 is as fo...

Embodiment 3

[0041] A 50.0ng / L PCB77 solution and an interfering substance solution with a concentration 100 times that of PCB77 were prepared, and the interfering substances were PCB81, p-nitrophenol, lead ions, mercury ions, nitrobenzene, and copper ions respectively. The selectivity of nucleic acid aptamer-nickel ferricyanide nanoparticles-RGO electrode to PCB77 was investigated. Using the test conditions in Example 2, the above solutions were respectively added into the test system, reacted for 60 min, and the peak current was recorded by differential pulse voltammetry. The relative change of its peak current is as Figure 5 As shown, the nucleic acid aptamer-nickel ferricyanide nanoparticles-RGO electrode is tested against PCB77 (the first bar graph), and secondly, the electrode is tested one by one for the interfering substances (the second to seventh bar graphs). The results show that the relative change of the current of the prepared electrode is only 53.7% when detecting PCB77. ...

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Abstract

The invention relates to an aptamer-nickel-iron cyanide nanoparticle-RGO electrode preparation method and electrochemical analysis method thereof for PCB77. The method is characterized by co-depositing nickel-iron cyanide nanoparticles with a diameter of about 5 nm onto the surface of reduced graphene oxide (RGO) in situ, modifying the obtained material on the surface of a gold electrode, and then, fixing a PCB77 aptamer to the nickel-iron cyanide nanoparticle-RGO composite material through covalent bonding reaction to obtain an aptamer-nickel-iron cyanide nanoparticle-RGO electrode. The prepared electrode is used for rapid and real-time detection of PCB77 in the environment. Compared with an existing PCB77 detection method, the method realizes high-sensitivity and high-selectivity detection of inert molecule PCB77 through combination of a high-sensitivity electrochemical analysis method and the aptamer having high specificity recognition capability; and detection limit can reach 0. 22ng / L. The method has the advantages of simple device and instrument, low analysis cost and being fast and simple and convenient and the like.

Description

technical field [0001] The invention relates to an electrochemically modified electrode, in particular to a nucleic acid aptamer-nickel ferricyanide nanoparticle-reduced graphene oxide (RGO) electrode and a preparation method thereof, and an electrochemical analysis method of the electrode to PCB77. [0002] technical background [0003] Polychlorinated biphenyls (PCBs) can exist stably in the environment for a long time through waste discharge during production and leakage, volatilization and incineration during use, and participate in the surface cycle, and then accumulate in the biological environment through the food chain. body and the human body. Chronic low-dose PCBs exposure can cause liver damage, reproductive abnormalities, nervous and endocrine system disorders and other problems. Therefore, PCBs are listed as the first class of pollutants to be controlled by the Stockholm Convention on Persistent Organic Pollutants. Toxicologists have found that some dioxin-like...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 范丽芳王贵珍梁桂芳梁文婷刘雪峰郭玉晶董川
Owner SHANXI UNIV
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