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High-magnetic micro-particle-size nano magnetic beads and preparation method thereof

A technology of nano-magnetic beads and magnetic nanoparticles, applied in the field of DNA detection, can solve the problems of low magnetic response and small specific surface area, achieve uniform particle size, small particle size, and promote the effect of separation and enrichment process

Active Publication Date: 2019-07-23
艾铂图生物(江苏)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to propose a nano-magnetic bead with high magnetic particle size and its preparation method, aiming to solve the technical problems of large particle size, wide distribution range, small specific surface area and low magnetic response in the prior art.

Method used

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  • High-magnetic micro-particle-size nano magnetic beads and preparation method thereof
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  • High-magnetic micro-particle-size nano magnetic beads and preparation method thereof

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Embodiment Construction

[0039] Embodiments of the present invention will be described in detail below, examples of which are shown in the drawings, wherein like reference numerals designate like elements or elements having the same function throughout. The embodiments described below by referring to the accompanying drawings are exemplary and are intended to explain the present invention, but cannot be construed as limitations to the present invention. Based on the embodiments in the present invention, those skilled in the art do not make creative work premise All other embodiments obtained below all belong to the protection scope of the present invention.

[0040] In order to solve the above technical problems, the present invention proposes a method for preparing nano magnetic beads with high magnetic particle size. refer to figure 1 As shown, the method includes the following steps:

[0041] Step S10, mixing ferric chloride, cobalt chloride and nickel chloride in equal proportions to obtain a me...

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Abstract

The invention discloses a preparation method of high-magnetic micro-particle-size nano magnetic beads. The preparation method comprises the following steps: mixing iron chloride, cobalt chloride and nickel chloride in an equal ratio to obtain a metal salt mixture; mixing ethylene glycol with the metal salt mixture sufficiently under the condition of mechanical stirring to obtain a mixture of the ethylene glycol and metal salt; adding sodium acetate into the mixture of the ethylene glycol and the metal salt, and continuing to stir to obtain a mixed solution; pouring the mixed solution into a non-metallic reaction kettle; placing the reaction kettle in a temperature-controlled oven, and controlling the oven to operate according to a preset procedure to make the mixed solution be subjected toa thermal reaction to form iron-cobalt-nickel alloy Fe-Co-NiOx magnetic nanoparticles; magnetically separating the iron-cobalt-nickel alloy Fe-Co-NiOx magnetic nanoparticles, and washing and drying;coating the surface layers of the iron-cobalt-nickel alloy Fe-Co-NiOx magnetic nanoparticles with a carbon layer and a silicon dioxide layer sequentially, and covalently grafting polyethylene glycol onto an outermost layer. The preparation method disclosed by the invention solves the technical problems of relatively large particle size, wide distribution range, small specific surface area and lowmagnetic response of magnetic beads in the prior art.

Description

technical field [0001] The invention relates to the field of DNA detection, in particular to a nano magnetic bead with a high magnetic particle diameter and a preparation method thereof. Background technique [0002] Cell-free DNA (nucleic acid) research is a hot field in genome molecular diagnosis research. It has been gradually applied clinically to all stages of tumors from initial diagnosis to treatment and development, including: early screening, evaluation of tumor heterogeneity, metastasis and recurrence Risk and prognosis assessment, real-time monitoring of treatment response and drug resistance, tumor staging and grading, and guidance of treatment options, etc. [0003] At present, the methods of extracting free DNA mainly include the magnetic bead method. The principle of the magnetic bead method to extract nucleic acid is: using superparamagnetic nanoparticles, using the method of absorbing nucleic acid under high-salt conditions and low pH, and then separating nu...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01D15/08B01J20/30
CPCB01D15/08B01J20/06B01J20/103B01J20/20B01J20/262B01J20/28009B01J20/28019
Inventor 张博葛东亮万昊赵淑婷张飞张海燕
Owner 艾铂图生物(江苏)有限公司
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