Urine microalbumin detection kit employing immunity transmission turbidity method

A technology of immunoturbidimetry and urine microalbumin, which is applied in the field of urinary microalbumin immune turbidimetry detection kits, can solve the problems of many operation steps and poor repeatability, and achieve cheap price, accuracy and stability Good, easy-to-use effect

Inactive Publication Date: 2019-06-28
济南腾奔生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the most widely used detection method for urine microalbumin in China is still

Method used

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  • Urine microalbumin detection kit employing immunity transmission turbidity method
  • Urine microalbumin detection kit employing immunity transmission turbidity method
  • Urine microalbumin detection kit employing immunity transmission turbidity method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A conventional urine microalbumin detection kit includes reagent R1 and reagent R2.

[0025] Wherein the reagent R1 consists of:

[0026]

[0027] Reagent R2 consists of:

[0028]

[0029] The reagents R1 and R2 described in this embodiment need to be prepared by adding buffer substances first, and then adding other substances after adjusting to an appropriate pH value. The kit described in the present embodiment, when in use, its measuring method is to adopt the Mindray 800 automatic biochemical analyzer with double reagent function, utilize the end-point method to measure, the main wavelength of detection is 415nm, the operation is as follows:

[0030] Add 15 μl of normal saline, sample or calibrator, then add 250 μl of R1 reagent, read the absorbance A1 after pre-incubation for 5 minutes, then add 50 μl of R2 reagent, read the absorbance A2 after reacting for 5 minutes, and calculate ΔA.

[0031] ΔA=A2-A1

[0032] Urinary microalbumin content (mg / L) = (ΔA me...

Embodiment 2

[0035] Accuracy verification test: the urine microalbumin detection reagent in Example 1 was used as the experimental group, and a urine microalbumin detection kit with good accuracy and good stability recognized on the market was used as the control group for detection, and 20 Clinical urine samples were tested, and the test results were as follows: image 3 shown. Correlation curves of the two reagents were obtained (e.g. figure 1 Shown), the results show that the correlation coefficient of the two groups of kits is 0.9991, indicating that the correlation between the two is better. It proves that the added and changed components of the kit of the present invention will not affect its accuracy, and the kit still maintains a good accuracy.

Embodiment 3

[0037] Linear correlation verification test: Select a high-value sample with a urine microalbumin content of 350mg / L, serially dilute it with normal saline, and prepare 6 samples with different concentrations, the concentrations are 350mg / L, 280mg / L, and 210mg / L , 140mg / L, 70mg / L, 0mg / L. Utilize respectively embodiment 1 reagent and control group reagent to detect, the sample of each concentration is measured three times respectively, get average value respectively, and detection result is as follows: Figure 4 shown.

[0038] Such as Figure 4 Shown, the correlation coefficient of embodiment 1 and the detection result of matched group reagent is all greater than 0.990, and the correlation coefficient of embodiment 1 reagent detection result is slightly greater than the correlation coefficient of control group reagent detection result, this shows that reagent of the present invention has better linear correlation sex.

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Abstract

The invention discloses a urine microalbumin detection kit employing an immunity transmission turbidity method, and belongs to the technical field of clinical in-vitro detection reagents. The kit comprises a reagent R1 and a reagent R2. EDTA-Na2 is added to the reagent R2, so the stability of the reagent is improved, the linear correlation is good, the accuracy of the reagent is also good, and thereagent can be further popularized and used in the market.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a urine microalbumin immune transmission turbidimetry detection kit. Background technique [0002] Microalbuminuria refers to the presence of microalbumin in the urine, which is a normal protein in the blood, but only very small amounts of albumin appear in the urine under physiological conditions. Microalbuminuria can reflect abnormal protein leakage from the kidneys. Increased urinary microalbumin is more common in diabetic nephropathy, hypertension, and preeclampsia, and is an early sensitive indicator of kidney damage. Urinary microalbumin indicators are usually used to monitor the occurrence of nephropathy, and the detection of urinary microalbumin is the most sensitive and reliable diagnostic index for early detection of nephropathy. [0003] Urinary microalbumin can evaluate the degree of kidney damage: when the urine microalbumin is found to ...

Claims

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Application Information

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IPC IPC(8): G01N21/82G01N21/31G01N33/68
Inventor 罗维晓王晨光胡晓飞
Owner 济南腾奔生物技术有限公司
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