A kind of method and its application of rapid detection acid aldehyde content in oil

A technology for rapid determination of oleic acid, applied in the field of analysis and detection, can solve the problems of time-consuming accuracy and sensitivity, complex carbonyl valence derivatization, and tedious pretreatment, and achieves the effects of reliable and high results, fast reaction speed, and easy promotion.

Active Publication Date: 2020-03-24
JIANGNAN UNIV
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  • Description
  • Claims
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Problems solved by technology

[0007] For the determination of oil rancidity in the prior art, the derivatization of the determination of the carbonyl value is more complicated, and the heating reaction is required for at least 30 minutes; the titration method is commonly used for the determination of the acid value, but it is time-consuming and has low accuracy and sensitivity; The determination of acid value requires cumbersome pretreatment, and the equipment is expensive and not easy to popularize.

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  • A kind of method and its application of rapid detection acid aldehyde content in oil
  • A kind of method and its application of rapid detection acid aldehyde content in oil
  • A kind of method and its application of rapid detection acid aldehyde content in oil

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Embodiment 1

[0060] The ratio of embodiment 1 chromogenic agent and fading agent

[0061] Take seven 15mL colorimetric tubes, and number them as 1, 2, 3, 4, 5, 6, and 7, add 0.1mL, 2mM chromogenic reagent solution in each, and then add them to 1~7 colorimetric tubes in sequence Add 0.2mL of sodium bisulfite solution (discoloring agent, concentrations are 240mM, 120mM, 100mM, 80mM, 60mM, 40mM, 20mM), dilute to 10mL with ethanol, and measure the relationship between absorbance and time as figure 2 shown.

[0062] It can be seen that the greater the concentration of sodium bisulfite, the better the fading effect, and No. 6 and No. 7 cannot make the color developer fade because the concentration of sodium bisulfite is too low. After the color is completely faded, add the acid-aldehyde mixed solution to be a mixed solution of 60-2mM hydrochloric acid and hexanal, and observe the color development of the solution after fully reacting, such as image 3 As shown, it can be known that the No. 1 ...

Embodiment 2

[0063] The influence of embodiment 2 temperature on fading

[0064] Take 1mL, 2mM developer solution, add 2mL, 60mM sodium bisulfite solution to fade the color, and place them in water baths at 5, 10, 20, 25, 30, 35, 40, and 45°C in sequence. It can be found that when the temperature When the temperature is above 40°C, it can fade within 15 minutes. When the temperature is higher than 40°C, the stable structure obtained by the reaction of sodium bisulfite and the color developer will be destroyed and the color cannot be completely faded. Therefore, the color fading temperature is selected from 5 to 40°C, preferably from 5 to 30°C.

Embodiment 3

[0066] R in chromogen I 1 , R 2 , R 3 Both are H atoms, R 4 , R 5 , R 6 It is methyl, and the decolorizing agent is sodium bisulfite. After the color is completely faded, the standard curves of acid and aldehyde are respectively drawn at the maximum wavelength. Assay mixture standard curve was used for calibration. The standard curve equations for acids and aldehydes are as Figure 4 (a) and Figure 4 (b) shown.

[0067] Take 1-1.5g of almond oil oxidized at 120°C for different times, weigh it in a 10mL colorimetric tube, dilute to 2mL with chloroform, add 5mL of decolorization solution and 2mL of ethanol, and finally dilute to 10mL with chloroform. Measure the absorbance at 546nm and 494nm after developing color for 5min, and then according to Figure 4 Obtained standard curve, obtain almond oil respectively at 120 ℃, the change curve of acid and aldehyde content with time, such as Figure 5 shown.

[0068] At the same time, use the national standard titration meth...

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Abstract

The invention discloses a method for rapidly detecting content of acid and aldehyde in oil and an application of the method and belongs to the field of analysis and detection. By use of the characteristic that a color developing agent can react with sulfite to fade, a colorless structure after fading can be damaged after acid or aldehyde is added and the color developing agent recovers the original color, the content of acid and aldehyde in oil is detected with a standard curve and an absorbance determination method, so that whether oil is rancid is evaluated rapidly. According to the method,the detection process is rapid, simple and convenient without tedious pretreatment processes, a little color developing agent is used, the reaction speed is high, whether oil has oxidative rancidity can be rapidly evaluated, the detection cost is low, and detection is economical and feasible; the content of acid and aldehyde in oil can be determined under the room-temperature condition, secondaryoxidation is avoided, and the detection result is more accurate. In the method, the standard curve obtained through the same color developing agent can be applicable to multiple oils, and the result is reliable; in the determination process, all that is required is to determine the absorbance without expensive precise instruments, and the method is easy to popularize.

Description

technical field [0001] The invention relates to a method for quickly detecting the acid aldehyde content in oil and its application, belonging to the field of analysis and detection. Background technique [0002] Oils and fats undergo oxidation or hydrolysis under the influence of light, heat, metal catalysts, microorganisms and water to form lipid hydroperoxides (primary oxidation products). Lipid hydroperoxides are easily decomposed to form secondary oxidation products such as aldehydes, ketones, acids, esters, alcohols, etc. in the presence of high temperature or metals. These secondary oxidation products will seriously affect the flavor of oils and fats. The valence may cause human gastrointestinal discomfort, diarrhea and liver damage, and the content of aldehydes will bring some unpleasant smells, so in the process of oil rancidity, the determination of the total acid aldehyde content in the process of oil oxidation is very important for evaluating the rancidity of oil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 朱振中向思敏王魏魏
Owner JIANGNAN UNIV
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