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Human cervical adenocarcinoma cell line and preparation method and application thereof

A technology for ovarian cancer cells and ovarian cancer, applied in the field of cell biology, can solve the problems of limited research, chemotherapy failure of chemotherapy drugs, etc., and achieve the effects of weak migration ability, stable drug resistance multiple, and shortened culture cycle

Active Publication Date: 2019-06-25
TIANJIN MEDICAL UNIV CANCER INST & HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, in the process of chemotherapy, the resistance of tumor cells to chemotherapy drugs is the main reason for the failure of chemotherapy.
Among the existing ovarian cancer cell lines, the lack of cell line models that are resistant to multiple chemotherapeutic drugs greatly limits the research in this field. Therefore, it is urgent to find a human ovarian cancer cell line to solve these problems

Method used

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  • Human cervical adenocarcinoma cell line and preparation method and application thereof
  • Human cervical adenocarcinoma cell line and preparation method and application thereof
  • Human cervical adenocarcinoma cell line and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Construction of Cell Lines

[0052] 1. Collection of Ovarian Ascites Samples

[0053] During the collection and transportation of liquid samples obtained from surgery, all reagents and instruments must be sterile, and they must be transported to the laboratory for processing within 6 hours after being separated from the body. Samples should be labeled "Ascites, peritoneal fluid, or peritoneal washings". And record the relevant information in the form. It is suggested that the patient’s name should not be recorded as the ID number, and the information on the form should be transferred to the experimental record book to ensure that the relevant clinical information is consistent with any tumor cell lines established from the sample.

[0054] 2. Pre-inoculation of primary ovarian cancer cells

[0055] 1) Under aseptic operation, transfer the ascites to a 50mL centrifuge tube with a sterile conical bottom, and centrifuge at 1000rpm at 4°C for 7min;

[0056] 2...

Embodiment 2

[0069] Example 2: Construction of Cell Lines

[0070] 1. Collection of Ovarian Ascites Samples

[0071] During the collection and transportation of liquid samples obtained from surgery, all reagents and instruments must be sterile, and they must be transported to the laboratory for processing within 6 hours after being separated from the body. Samples should be labeled "Ascites, peritoneal fluid, or peritoneal washings". And record the relevant information in the form. It is suggested that the patient’s name should not be recorded as the ID number, and the information on the form should be transferred to the experimental record book to ensure that the relevant clinical information is consistent with any tumor cell lines established from the sample.

[0072] 2. Pre-inoculation of primary ovarian cancer cells

[0073] 1) Under aseptic operation, transfer the ascites to a 50mL centrifuge tube with a sterile conical bottom, and centrifuge at 1000rpm at 4°C for 7min;

[0074] 2...

Embodiment 3

[0094] Example 3: Subculture and cryopreservation of cell lines

[0095] 1. Change the liquid

[0096] Inoculate the primary culture and change the medium with fresh screening medium every 3-4 days. When changing the medium, use a sterile Pasteur pipette to suck away the old medium, and then replace it with a new straw to add new complete medium.

[0097] 2. Avoid cross-contamination

[0098]To reduce or avoid the possibility of contamination of newly cultured cell lines with cells from other sources, use the following precautions:

[0099] If conditions permit, when the primary cultured cell line is established, the laboratory will not cultivate other established cell lines. If conditions do not permit, try to ensure that the newly established primary cultured cell line is cultured in an independent ultra-clean bench and incubator. The medium used is separately packed in a sterile container and marked separately. Each culture operation is carried out in a cell culture ultra...

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Abstract

The present invention provides a human cervical adenocarcinoma cell line, which is characterized by naming as a primary cervical adenocarcinoma drug-resistant strain W038, and is deposited in the China Center for Type Culture Collection with a preservation number being CCTCC No: C2017246. The primary cervical adenocarcinoma drug-resistant strain W038 has weak invasion ability and weak migration ability, and can be used as a double-negative control group for invasion and migration. The in-vitro soft agar colony formation test proves that the tumorigenicity proves that the cloning ability is strong, and the in-vitro susceptibility test that the strain is resistant to a variety of drugs, which is consistent with clinical features. The preparation method disclosed by the invention can purify cancer cell lines to over 99%, wherein the screened medium component can selectively kill histocyte, has high selectivity for tumor cell growth, and can increase the proliferation rate of tumor cells and shorten the culture period. The method can be used not only to purify the cervical adenocarcinoma lines, but also to purify and prepare other cancer cell lines.

Description

technical field [0001] The invention relates to the field of cell biology, in particular to a human ovarian cancer cell line and its preparation method and application. Background technique [0002] Ovarian cancer is one of the three major malignant tumors of the female reproductive system. The incidence of ovarian cancer in different countries ranges from 1 / 100,000 to 17 / 100,000. The incidence of ovarian cancer in my country is second only to cervical cancer and uterine body cancer, ranking third. . Ovarian cancer is the second most common malignancy in women in the United States after uterine corpus cancer. Due to the lack of effective early diagnosis methods, most patients are diagnosed at an advanced stage when symptoms appear. The death rate of epithelial ovarian cancer ranks first among gynecological malignancies. Although patients improved significantly after surgery and chemotherapy, the long-term prognosis remained poor. Most patients (75%-80%) are diagnosed with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02C12R1/91
Inventor 王珂于敏
Owner TIANJIN MEDICAL UNIV CANCER INST & HOSPITAL
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