Method for enhancing tobacco cut stem fermentation process

A technology of fermentation process and shredded tobacco stems, which is applied in the field of microorganism and tobacco processing, can solve the problems of affecting product acceptance and reducing aroma, and achieve the effect of softening smoke, increasing smoke aroma, and improving aroma

Inactive Publication Date: 2019-06-21
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of shredded stems as auxiliary materials in snus will also bring unfavorable factors that reduce the aroma. Therefore, the use of biological fermentation methods to improve the quality of shredded stems is a key factor in determining the style characteristics of snus products and reducing prices, thereby affecting the consumer market. acceptance of the product

Method used

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  • Method for enhancing tobacco cut stem fermentation process
  • Method for enhancing tobacco cut stem fermentation process
  • Method for enhancing tobacco cut stem fermentation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This example is the identification of the isolated Bacillus amyloliquefaciens GZU03.

[0029] The Bacillus amyloliquefaciens GZU03 colony and microscope morphology observation: figure 1 It is a microscope (1000X) observation photo of Bacillus amyloliquefaciens GZU03, the gram-stained bacterial body of the bacterial strain is purple, and is a gram-positive bacterium. figure 2 It is the plate colony form of Bacillus amyloliquefaciens GZU03. On the casein plate, it can produce hydrolysis circles and form round, low concave, sticky small colonies. Its color is milky white or light yellow, with regular edges and no luster. , slightly elevated, opaque, colony diameter 2.0mm ~ 3.0mm.

[0030] The entire 16S rRNA sequence of the Bacillus amyloliquefaciens GZU03 is shown in the sequence table.

[0031] Physiological and biochemical characteristics of the Bacillus amyloliquefaciens GZU03 are identified, the physiological and biochemical characteristics of the Bacillus amyloliq...

Embodiment 2

[0042] The present embodiment is the preparation method of Bacillus amyloliquefaciens GZU03 preparation, specifically comprises the following steps:

[0043] (1) Weigh 1.25g of tryptone, 0.625g of yeast extract, 6.25g of NaCl, and 0.625g of glucose, add distilled water to 1000mL, and sterilize by high-pressure steam at 121°C for 20min, and insert the Bacillus amyloliquefaciens after cooling GZU03 was cultured at 37°C and 180r / min for 18h±2h to obtain seed liquid;

[0044] (2) centrifuge 1000mL of the seed liquid at 4°C and 8000r for 10min, collect the precipitate, and wash the precipitate with 8.5g / L sterilized physiological saline, then add 1000mL of sterilized physiological saline to the precipitate, Shake well to obtain the Bacillus amyloliquefaciens GZU03 preparation.

[0045] The sterilized physiological saline needs to be sterilized by high pressure steam at 121° C. for 20 minutes.

Embodiment 3

[0047] In the present embodiment, take 20g cut tobacco stems by weighing in a sealed bag with a size of 14cm×20cm, draw 2mL of the Bacillus amyloliquefaciens GZU03 preparation (i.e. 10% inoculation amount) on the cut tobacco stems, and put 4mL of the sugar-salt solution on the tobacco stems Silk, fully mixed to obtain sample 1.

[0048] The preparation steps of the sugar-salt solution are as follows: Weigh the MgCl 2 5g, KCl 0.5g, CaCl 2 0.5g, 0.5g NaCl, 2g glucose, add water to 1000mL, dissolve to obtain a sugar-salt solution.

[0049] Weigh 20g of cut tobacco stems into a sealed bag with a size of 14cm×20cm, draw 2mL of sterile physiological saline and 4mL of sugar-salt solution into the cut tobacco stems, and mix well to obtain Comparative Sample 1.

[0050] The sample 1 and the control sample 1 were cultivated in the incubator at 37°C at the same time, and the ventilation was carried out regularly every day, and when the humidity decreased, a sugar-salt solution must b...

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Abstract

The invention discloses a method for enhancing a tobacco cut stem fermentation process. A bacillus amyloliquefaciens GZU03 preparation is used for processing tobacco cut stems in the fermentation process, and is prepared from bacillus amyloliquefaciens GZU03. The bacillus amyloliquefaciens GZU03 is preserved in CCTCC (China Center for Type Culture Collection) on November 9, 2018 and the preservation number is CCTCC M 2018762. The method for enhancing the tobacco cut stem fermentation process, disclosed by the invention, has the beneficial effects that the bacillus amyloliquefaciens GZU03 preparation can enhance the tobacco cut stem fermentation process, and has the effects of increasing cigarette aroma, softening smoke, increasing exquisiteness, reducing irritation, obviously improving smoke quality and improving the practical safety of snus. Meanwhile, the invention further discloses a method for improving the activity of protease and amylase in the tobacco cut stem fermentation process.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and tobacco processing, and in particular relates to a method for strengthening the fermentation process of shredded tobacco stems. Background technique [0002] Smokeless tobacco cut stem products (Smokeless tobacco products, STPs) do not burn when used, do not produce harmful pyrolysis products, produce no smoke, and can be used in public places. They are low-harm and environmentally friendly new cut tobacco stem products. An important form of composition. [0003] Snus is an important product form of STPs, and it is the product category with the fastest growing market share. It has attracted the attention of major cut tobacco stem companies in the world, and has invested a lot of money and energy in related research and development in this field. [0004] Among the raw materials for snus products, tobacco stem is an important auxiliary material component, which is generally used in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/18C12N1/20C12R1/07
Inventor 田永峰杨柳王昆淼朱瑞芝段沅杏赵伟赵杨韩熠张霞刘志华陈永宽缪明明
Owner CHINA TOBACCO YUNNAN IND
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