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Dual-mode immunoassay method of ovarian cancer tumor marker

A tumor marker and immune analysis technology, which is applied in the field of dual-mode immune analysis of ovarian cancer tumor markers, to achieve the effect of improving photoelectric conversion efficiency and electrical conductivity

Active Publication Date: 2019-06-14
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, the more commonly used ovarian cancer biomarkers mainly include cancer antigen 125 (CA125) and human epididymis protein 4 (HE4), but the above two biomarkers are only suitable for the diagnosis of benign ovarian cancer

Method used

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  • Dual-mode immunoassay method of ovarian cancer tumor marker
  • Dual-mode immunoassay method of ovarian cancer tumor marker
  • Dual-mode immunoassay method of ovarian cancer tumor marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A dual-mode immunoassay for ovarian cancer tumor markers (e.g. figure 1 shown):

[0033] (1) Indium tin oxide (ITO) electrode pretreatment of indium tin oxide (ITO) electrode: first put the indium tin oxide (ITO) electrode in an ultrasonic water bath to clean until it is clean, and then use ethanol, dilute acid and Wash thoroughly with water;

[0034] (2) Add dropwise 0.6mL of carbon nanohorn suspension with a concentration of 3mg / ml on the surface of a clean ITO electrode, dry under infrared light, and cool to room temperature;

[0035] (3) 0.6 mL of anatase TiO with a concentration of 3 mg / mL was sequentially modified on the surface of the above electrode 2 Mesoscopic crystal suspension and 0.6 mL of rutile TiO at a concentration of 3 mg / mL 2 The mesoscopic crystal suspension was dried under an infrared lamp and cooled to room temperature, and this was repeated three times to obtain a CNHs / Anatase / Rutile modified electrode;

[0036] (4) Mix the mercaptophenylboron...

Embodiment 2

[0045] A dual-mode immunoassay method for ovarian cancer tumor markers, the steps are as follows:

[0046] (1) The three-electrode system was used for the measurement. The CNHs / Anatase / Rutile / Cu NCs / Ab / Ag modified electrode prepared in Example 1 was used as the working electrode, Ag / AgCl was used as the reference electrode, and the platinum wire electrode was used as the counter electrode. Use a photoelectrochemical workstation for detection, set the voltage to 0.1V, switch the lamp every 10s, and the monochromatic light excitation light emitted by the xenon lamp is filtered by the monochromator before use;

[0047] (2) In a PBS solution with a concentration of 0.3mol / L at pH=7.5, detect 1×10 by photoelectrochemical workstation -6 ng / mL–10 ng / mL a series of lipolysis-activated lipoprotein receptor standard solutions with different concentrations, and draw the working curve by recording the different current signals generated before and after switching the light. Figure 2 A is...

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Abstract

The invention discloses a dual-mode immunoassay method of an ovarian cancer tumor marker. A sensing interface is constructed by taking a carbon nanohorn and copper nano-cluster functionalized mixed phase (anatase / rutile) titanium dioxide mesoscopic crystal as a substrate, therefore, a lipolysis activated lipoprotein receptor antibody (Ab) (an early ovarian cancer tumor marker antibody) is immobilized; a free lipolysis activated lipoprotein receptor (an early ovarian cancer tumor marker) can be specifically recognized and combined with the immobilized lipolysis activated lipoprotein receptor antibody to the sensing interface, so as to cause the weakening of a photocurrent signal of an electrode and weaken the capability of the electrode to catalyze and decompose hydrogen peroxide; and hydrogen peroxide solutions with different concentrations can also oxidize and reduce methylene blue so as to enable the methylene blue to present blue with different chromaticities. Based on the phenomenon, a dual-mode analysis method for the colorimetric and photoelectrochemical response of the lipolysis activated lipoprotein receptor can be established.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and in particular relates to a dual-mode immunoassay method for ovarian cancer tumor markers. Background technique [0002] Photoelectrochemical (PEC) detection uses light as the excitation signal and photocurrent as the detection signal. By using different forms of energy as the excitation signal and detection signal, the excitation and detection signals do not interfere with each other, so the background signal is low and can be obtain higher sensitivity. In the construction process of photoelectrochemical sensors, the choice of photosensitive materials is crucial to the response of the signal. Among the currently used materials, TiO 2 Nanomaterials are ideal materials for photocatalytic and photoelectrochemical sensors because of their unique photocatalytic activity, non-toxicity, and excellent chemical and physical stability. TiO 2 Mesoscopic cry...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N21/78G01N27/30G01N27/327G01N27/416
Inventor 戴宏陈妍洁张书培衣欢宋建榕郑祥钦
Owner FUJIAN NORMAL UNIV
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