A method for rhamnolipid modified biochar to strengthen Spartina to remediate oil-contaminated soil
A rhamnolipid and oil-polluted technology, applied in the restoration of polluted soil, carbon preparation/purification, etc., can solve the problems of low bioavailability of organic pollutants, increase of petroleum hydrocarbons, low biological efficiency, etc., and achieve recovery And improve the effect of biodiversity, strong nitrogen fixation ability, and more rhizosphere microorganisms
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[0029] (1) The rice husk was repeatedly washed with deionized water to remove surface dust, and dried in a drying oven at 60° C. for 12 hours. Crush the washed and dried rice husks to a particle size of about 2 mm, place the crushed rice husks in a ceramic crucible (full), and use N 2 Close the lid immediately after removing the air. After sealing the crucible and lid with tinfoil paper, pyrolyze in a muffle furnace at 700°C for 3 hours with limited oxygen. The biochar obtained by pyrolysis was passed through a 50-mesh sieve, washed with 1mol / L HCl solution, and then washed repeatedly with deionized water until the pH of the biochar solution was close to neutral. Put the washed biochar in an oven, dry it at 80°C, and store it in a glass sample bottle, which is recorded as 700BC.
[0030] (2) Prepare 10mmol / L rhamnolipid aqueous solution, then add the biochar obtained in 10g step (1) and 50mL rhamnolipid aqueous solution into a 100mL Erlenmeyer flask, place on a magnetic stir...
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