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Method for measuring 24 mycotoxins in infant nourishing rice flour

A technology for nutritious rice flour and a determination method, applied in the field of food analysis, can solve the problems that strong polar mycotoxins cannot be extracted at the same time, serious matrix effect, low sensitivity, etc., and achieve the effects of low cost, reduced matrix effect, and fast analysis speed.

Inactive Publication Date: 2019-05-21
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention aims at problems such as high cost, low sensitivity, serious matrix effect, fumonisin and other strong polar mycotoxins that cannot be extracted simultaneously in the existing pretreatment technology of infant nutritional rice flour, which is subjected to high-temperature puffing process, and provides a A Method for the Simultaneous Determination of 24 Mycotoxins in Infant Nutritional Rice Flour Using QuEChERS-PRIME HLB Purification Technology

Method used

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  • Method for measuring 24 mycotoxins in infant nourishing rice flour
  • Method for measuring 24 mycotoxins in infant nourishing rice flour
  • Method for measuring 24 mycotoxins in infant nourishing rice flour

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Embodiment 1

[0036] Example 1

[0037] 1. Instruments and materials

[0038] AB SCIEX Triple Quad 5500 Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometer (with Electrospray Ionization Source); Chromatographic Column: Waters ACQUITY UPLC HSS T 3 Chromatographic column (100mm×2.1mm, 1.8μm), BT 125D electronic balance (Sartorius, Germany); MS3 vortex mixer (IKA, Germany); SB-800DTD ultrasonic cleaner (Ningbo Xinzhi Biotechnology Co., Ltd.) ; Prepared by Mili-Q ultrapure water machine.

[0039] Aflatoxin mixed standard (AFB1, AFB2, AFG1, AFG2), aflatoxin M1, ochratoxin A, deoxynivalenol, zearalenone (O2si company in the United States); aflatoxin M2, Fumonisin B3, HT-2 toxin, 3-acetyl deoxynivalenol, 15-acetyldeoxynivalenol (Beijing Manhag Company); fumonisin B1, fumonisin Ochratoxin B2 (Canada TRC Company); Ochratoxin B, Versicolor, Zearalane (Israel Fermentek Company); T-2 toxin (US ROMER Company); O-methyl Versicolor ( Shanghai Zhenzhun Biotechnology Co., Ltd.); α-zear...

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Abstract

The invention discloses a method for measuring 24 mycotoxins in infant nourishing rice flour. Related target compounds comprise aflatoxin, nivalenol, fumonisin, ochratoxin, T-2 toxin and HT-2 toxin, zearalenone and a derivative thereof, sterigmatocystin and O-methylsterigmatocystin. The method comprises the steps of adding a mixed extraction solvent which is composed of acetonitrile, formic acid and water into rice flour; successively adding MgSO4 and a sodium acetate composite salt bag for enriching an object into an organic layer, performing purification by means of PRIME HLB and performingnitrogen blowing to approximately dry, performing redissolving and passing a film, and performing detection and analysis by an ultrahigh-performance liquid chromatography-serial mass spectrometer. Themethod according to the invention has advantages of simple and economical pre-treatment, time-saving and labor-saving effect, high sensitivity of the method, low detecting limit, high recovery rate,high flux and low matrix effect. The method is suitable for high-flux detection for mycotoxins in the infant nourishing rice flour.

Description

technical field [0001] The invention belongs to the technical field of food analysis, and relates to a method for determining 24 kinds of mycotoxins in infant nutrition rice noodles, in particular to the determination of 24 kinds of mycotoxins in infant nutrition rice noodles based on QuEChERS purification technology-ultra-high performance liquid chromatography tandem mass spectrometry Methods. Background technique [0002] Mycotoxins are a class of biotoxins widely present in grains and their products, and are toxic secondary metabolites produced by fungi. Because the toxicity of mycotoxins varies greatly, the target organs of action are also different. Mild mycotoxin poisoning can cause nausea, vomiting, dizziness, diarrhea, etc., and severe cases can lead to liver and kidney failure, and even death. Different mycotoxins produce different substrates and often exist in multiple substrates. For example, corn is often contaminated by various mycotoxins such as zearalenone,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/54
Inventor 郑红于文江尹丽丽孙立臻刘艳明薛霞王骏周传静孙姗姗赵慧男朱辉
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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