Environment-friendly biological oil extraction method

An environmentally friendly and biological technology, applied in the field of bio-flooding, can solve the problems of reduced flow rate of heat sources, lack of large-scale promotion, and poor application conditions of chemical agents, so as to improve crude oil recovery, improve activation effect, reduce The effect of cost input

Active Publication Date: 2019-05-21
环能新科技术服务(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are obvious defects: in the middle and late stages, the effect will be significantly reduced due to factors such as excessive heat source flow, fingering phenomenon, and deterioration of formation conditions; the cost is too high, and it is not suitable for large-scale promotion
At present, although chemical flooding technology is widely used, it is faced with poor application conditions of chemical agents (temperature and salt resistance, groundwater salinity, etc.), high construction technology requirements, difficult oil-water separation, difficult sewage treatment, and There are many challenges such as serious damage to the formation; especially when the energy such as disturbance cannot enter the ground, it is difficult to form sufficient emulsification
Gas flooding is a technology that injects a certain amount of carbon dioxide, nitrogen or hydrocarbon gas into the oil reservoir to increase oil recovery by reducing the viscosity of crude oil, expanding the volume of crude oil, and forming miscible or immiscible flooding. It is a deep, low-permeability technology. Effective recovery technology for oil reservoirs, but this technology has high cost, low sweep efficiency, low recovery factor, and is not suitable for large-scale use

Method used

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  • Environment-friendly biological oil extraction method
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  • Environment-friendly biological oil extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Nutrient ingredient screening experiment and analysis experiment.

[0051] (1) Take an appropriate amount of crude oil from the oil field test area, and use glycerin, yeast extract, ethanol, sucrose, molasses, MgSO 4 , MgCl, KCl, K 2 SO 4 , NH4Cl, NaNO 3 , (NH 4 ) 2 HPO 4 、K 2 HPO 4 、Na 2 SO 4 , FeSO 4 , CaCl 2 , CaCO 3 , NaCl and Na 2 MoO 4 Select nutrients and cultivate them separately:

[0052] ①Add glycerin, yeast extract and ethanol to the crude oil, divide it into 2 parts, add sucrose or molasses to each part respectively, and culture on a shaker at 60°C and 200rpm for 10 days, measure the emulsification phenomenon and discharge of crude oil in different culture systems. The size of the oil circle and the concentration of the bacteria, compared and screened out the suitable ingredient to be molasses;

[0053] ② Add glycerin, yeast extract, ethanol and molasses to the crude oil, divide it into 2 parts, and add MgSO to each part respectively 4 Or Mg...

Embodiment 2

[0064] Strain acquisition.

[0065] (1) Take an appropriate amount of reservoir formation water and insert it into the enrichment medium, which is an inorganic salt medium: (NH 4 ) 2 SO 4 4.5g / L, starch 2.0g / L, Na 2 HPO 4 2.0g / L, MgSO 4 ·7H 2 0.5g / L, NaCl1.5g / L, FeCl 2 0.5g / L, 0.3g / L yeast powder, 2.0g / L asphalt, 5.0g / L liquid paraffin, pH 7.2-7.5, sterilized at 121°C, cultured on a shaker at 60°C at 200rpm for 15-20 days.

[0066] (2) Gradually dilute the enriched culture solution in step (1), and evenly spread the bacterial solutions with dilution factors of 104 and 105 on the LB plate medium. Cultivate at 60°C for 1 to 2 days, select the cultured colonies, transfer them to liquid LB medium for expansion, insert the expanded seeds into the above medium for 20 days, and select single bacteria with the ability to emulsify liquid paraffin save.

[0067] Pick the single colonies screened by the above method and inoculate them in the crude oil fermentation medium (waste ...

Embodiment 3

[0072] Production of bioactives and determination of their capacity.

[0073] (1) Inoculate CGMCC No.14770 strains in LB liquid medium with 0.5% crude oil, culture at 62°C at 200r / min for 24 hours, then inoculate the bacteria in 1L of different crude oil medium at 62°C at 200r / min Carry out aerobic and anaerobic shaker culture for 10 days; medium 1 includes crude oil 10g / L, molasses 2 / L, NaNO 3 2g / L, yeast extract 1g / L; K 2 HPO 4 1.2g / L, MgSO 4 0.5g / L, KCl0.5g / L, CaCO 3 0.02g / L,Na 2 MoO 4 0.01g / L, ethanol 0.01%, FeSO 4 0.01-0.05g / L, pH7.0~8.0, medium 2 includes crude oil 8g / L, molasses 2 / L, NaNO 3 2g / L, yeast extract 1g / L; K 2 HPO 4 1.2g / L, MgSO 4 0.5g / L, KCl0.5g / L, ethanol 0.01%, FeSO 4 0.01-0.05g / L, pH7.0-8.0. After the cultivation was completed, the fermentation broth was taken out and heated in a water bath at 60°C for 10 minutes, centrifuged at 10,000 g for 20 minutes to remove the bacteria, and the supernatant of the fermentation broth was taken, and after ex...

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Abstract

The invention discloses an environment-friendly biological oil extraction method, comprises the steps of injecting a biological activator into an oil field test area by using a high-pressure plunger pump through a water injection well, wherein the injection amount of the biological activator is 0.05 to 0.1 PV, the injection speed is 80-150 m<3> / d, the air injection amount is 2-3*10<5> Nm<3> / d, theinjection mode is continuous injection, the biological activator is prepared by mixing glycerol, molasses, NaNO3, yeast extract, K2HPO4, MgSO4, KCl, ethanol, FeSO4, CaCO3 and Na2MoO4, and the components and the weight ratio of the biological activator are determined through a screening experiment and an analysis experiment. The crude oil recovery rate can be improved, beneficial activities and metabolites of microorganisms are utilized, through the effects of degrading crude oil, reducing viscosity, increasing local pressure, increasing fluidity, changing oil-water interfacial tension, improving rock wettability and the like, remaining oil is further extracted, the technological content is high, economic benefits are good, and environmental protection and no pollution are achieved.

Description

technical field [0001] The invention belongs to the technical field of biological oil flooding, and in particular relates to an environment-friendly biological oil recovery method. Background technique [0002] Crude oil is crude oil with high asphaltene and colloid content and high viscosity. Its reserves exceed conventional crude oil and it is a very important strategic resource. The reason for the high viscosity of crude oil is the macromolecular stacking entanglement of heterocyclic and polycyclic aromatic hydrocarbons. The key problem in the development of crude oil is the reduction of viscosity or freezing point, that is, to destroy the structure or interaction of heterocyclic and polycyclic aromatic hydrocarbons in crude oil, or to reduce the content of heavy components. At present, crude oil development methods are thermal recovery, gas recovery, or adding organic solvents or chemical agents, etc. However, these methods are very expensive and pollute, and can only b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E21B43/22C12N1/38C12N1/20C12Q1/689C12Q1/04C12R1/07
Inventor 罗楚平夏文杰张勇李雪
Owner 环能新科技术服务(北京)有限公司
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