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A Bletilla striata polysaccharide-carboxymethyl chitosan composite hydrogel and its preparation

A technology of carboxymethyl chitosan and composite hydrogel, applied in bandages, medical science, etc., can solve the problems of high price and poor spreadability, and achieve good adhesion, strong water retention and biocompatibility Good results

Active Publication Date: 2021-07-09
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are human epidermal reorganizing factor hydrogels for wound repair on the market, but the price is relatively expensive.
There are also studies reporting chemically cross-linked hydrogel dressings, but their spreadability is poor, and the chemical cross-linking agents used have certain cytotoxicity.

Method used

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  • A Bletilla striata polysaccharide-carboxymethyl chitosan composite hydrogel and its preparation
  • A Bletilla striata polysaccharide-carboxymethyl chitosan composite hydrogel and its preparation
  • A Bletilla striata polysaccharide-carboxymethyl chitosan composite hydrogel and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of Bletilla striata polysaccharide-carboxymethyl chitosan composite hydrogel

[0037] Process flow chart such as figure 1 As shown, it mainly includes the following steps:

[0038] (1) Preparation of Bletilla striata polysaccharide solution: Weigh 1 g of Bletilla striata polysaccharide and dissolve it in 10 mL of deionized water to prepare Bletilla striata polysaccharide solution;

[0039] (2) prepare carboxymethyl chitosan solution: take by weighing 1g carboxymethyl chitosan and be dissolved in 10mL deionized water, make carboxymethyl chitosan solution;

[0040] (3) Preparation of Carbomer 940 gel matrix: Weigh 0.5g of Carbomer 940 and dissolve it in 40mL of deionized water, and wait for it to fully swell overnight at room temperature;

[0041] (4) Bletilla striata polysaccharide solution in the present embodiment: carboxymethyl chitosan solution: the proportioning of Carbomer 940 is respectively 1. 4:1:1, 2. 4:1:2, 3. 1:2:1.

[0042] (5) Preparation of ...

Embodiment 2

[0046] Structural Characterization Determination

[0047] Scanning electron microscope detection: Put Bletilla striata polysaccharide-carboxymethyl chitosan composite hydrogel into a 24-well plate, freeze at -40°C for 24 hours, break off, and dry in vacuum for 48 hours. The freeze-dried sample was pasted on the conductive adhesive, and sprayed with gold for 20 minutes in a vacuum environment. The cross-sectional microstructure was observed through a scanning electron microscope at a voltage of 10KV.

[0048] Fourier transform infrared spectrum detection: Bletilla striata polysaccharide, carboxymethyl chitosan, carbomer 940 and a small amount of KBr were mixed and ground respectively, and pressed into thin slices. Freeze-dried gel samples were analyzed by Fourier transform infrared spectroscopy at a wavelength of 500-4000 cm with the aid of the ATR accessory. -1 range to scan.

[0049] X-ray single crystal diffractometer detection: Grind the freeze-dried gel sample; respecti...

Embodiment 3

[0052] Evaluation of physical and chemical performance indicators

[0053] Rheological performance test: use M101 rheometer to test the storage modulus (G'), loss modulus (G") and dynamic complex viscosity (η') of the composite hydrogel. Parameters: temperature 37°C, frequency 0.01 -10HZ.

[0054] Water loss test: Take a certain mass of composite hydrogel in a 2mL EP tube, and accurately weigh its initial mass (W 0 ). Put the composite hydrogel sample in a silica gel desiccator, place it at 37°C for 18h, take it out every 1h and accurately weigh the mass (W t ). The water loss rate is calculated by the following formula:

[0055]

[0056] The results clarified the physicochemical properties of Bletilla striata polysaccharide-carboxymethyl chitosan composite hydrogel, in which, Figure 6 It shows that the storage modulus of the composite hydrogel is much greater than the loss modulus, that is, the composite hydrogel has solid-state elastic characteristics, and the stora...

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Abstract

The invention relates to a Bletilla striata polysaccharide-carboxymethyl chitosan composite hydrogel and its preparation. The preparation mainly includes: first configuring a Bletilla striata polysaccharide solution and a carboxymethyl chitosan solution, The proportion is mixed with fully swollen carbomer 940, triethanolamine is used to adjust the pH of the gel system, and then a certain amount of bletilla striata polysaccharide solution and deionized water are added, and fully stirred to obtain a complex polysaccharide hydrogel. The prepared Bletilla striata polysaccharide-carboxymethyl chitosan composite hydrogel is formed by physical cross-linking of hydrogen bonds, has a porous structure and an amorphous structure, and its rheological properties show solid-state elastic characteristics and good water retention; It has good biocompatibility and has obvious repairing effect on wound damage. The preparation method of the invention is simple, it is prepared by physical mixing under certain solution conditions, avoids the use of toxic crosslinking agents, the preparation process is safe and easy to operate, and the cost is low, and the raw materials are easy to obtain.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a bletilla striata polysaccharide-carboxymethyl chitosan composite hydrogel and its preparation. Background technique [0002] Accidents that occur in daily life, such as car accidents, burns, sports friction, etc., cause skin damage. The wound healing process is long and complicated, and there is a risk of bacterial infection and suppuration. Hydrogel dressings can maintain wound moisture; adhere to sensitive underlying tissue coverage; relieve pain and promote epithelial formation and cell migration to reduce the risk of scar formation, which has a positive impact on the wound healing process. At present, human epidermal reorganizing factor hydrogels for wound repair are available on the market, but they are more expensive. There are also studies reporting chemically cross-linked hydrogel dressings, but their spreadability is poor, and the chemical cross-linking agents us...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08J3/24C08L5/00C08L5/08A61L26/00
Inventor 刘杨黄滢蓓史福林杨宇肖湘伦镜盛
Owner SHANTOU UNIV
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