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Traditional Chinese medicine composition for adjuvant treatment of liver cancer and preparation method and application thereof

An adjuvant therapy and composition technology, applied in the field of medicine, can solve the problems of increased biotransformation and detoxification, allergic reactions, adverse reactions, etc., and achieve the effects of improving inhibition, increasing apoptosis, and good safety.

Inactive Publication Date: 2019-05-17
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to altered drug absorption and efflux, increased biotransformation and detoxification in the liver, the main reason limiting the use of cisplatin is drug resistance
At the same time, there are certain adverse reactions, including liver and kidney toxicity, neurotoxicity affecting vision and hearing, bone marrow suppression, allergic reactions, bleeding, etc.

Method used

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  • Traditional Chinese medicine composition for adjuvant treatment of liver cancer and preparation method and application thereof
  • Traditional Chinese medicine composition for adjuvant treatment of liver cancer and preparation method and application thereof
  • Traditional Chinese medicine composition for adjuvant treatment of liver cancer and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of Chinese medicine composition extract, it comprises the following steps:

[0027] Take astragalus 20g, curcuma 10g, flea 20g, agrimony 20g, asparagus 10g, coix seed 10g, flat wood 15g, diffusa 15g, pinellia 15g, sarsaparilla 15g, ganoderma lucidum 20g and rattan Combine 15 g of pear roots, add water with volume 10 times the weight of the medicinal materials, soak for 30 minutes, then extract by decoction twice, each time for 0.5 hours, filter, take the filtrate, combine the filtrate obtained by the two extractions, and concentrate to obtain 1.2g / mL crude drug concentration of the traditional Chinese medicine composition extract, stored in a 4 ℃ refrigerator for subsequent use.

Embodiment 2

[0029] The traditional Chinese medicine composition extract prepared in Example 1 was used to carry out the effect of combined use of the chemotherapeutic drug cisplatin on the growth and metastasis of orthotopic transplanted tumors in nude mice with human liver cancer, as follows:

[0030] 1. Experimental method

[0031] Subcutaneous implantation of liver cancer cells: Culture human liver cancer HepG2 cells to the logarithmic growth phase, digest and make 2.5×10 7 cells / ml of cell suspension. 0.2ml of tumor cell suspension was implanted subcutaneously in nude mice, and visible tumors appeared in about 1 week.

[0032] Establishment of orthotopic xenograft model of human liver cancer in nude mice: the subcutaneous tumor grows to about 1cm in about 3 weeks 3 The tumor-bearing nude mice were sacrificed immediately, the tumor body was removed under aseptic conditions, put into RPMI1640 culture medium, the connective tissue was removed, and the tumor tissue was divided into 1mm ...

Embodiment 3

[0051] Adopt the Chinese medicine composition extract prepared in the embodiment 1, carry out to the impact of combined use chemotherapy drug cisplatin on human liver cancer nude mice immune function and myelosuppression, specifically as follows:

[0052] 1. Experimental method

[0053] The modeling and drug administration groupings are the same as the implementation case 2.

[0054] Detection of spleen index: the spleen of nude mice was removed and weighed, spleen index=spleen mass (mg) / nude mouse body mass (g).

[0055] Peripheral blood leukocyte detection: blood is collected from the retrobulbar venous plexus, and about 0.2 mL of blood is taken, placed in an EDTA-K2 anticoagulant tube, mixed upside down 4-5 times, and then the peripheral blood leukocytes are counted using an automatic blood cell analyzer .

[0056] 2. Experimental results

[0057] 2.1 The effect of traditional Chinese medicine composition combined with cisplatin on the spleen index of nude mice with huma...

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Abstract

The invention discloses a traditional Chinese medicine composition for adjuvant treatment of a liver cancer. The traditional Chinese medicine composition is prepared from 10-20 parts of astragali radix, 5-15 parts of curcuma zedoary, 10-30 parts of manyleaf paris rhizome, 10-20 parts of hairyvein agrimony, 5-10 parts of radix asparagi, 5-10 parts of coix seeds, 15-30 parts of ardisiae japonica, 15-30 parts of oldenlandia diffusa, 15-30 parts of rhizoma pinelliae and 15-30 parts of chinaroot greenbrier, 10-40 parts of ganoderma lucidum and 15-30 parts of Chinese actinidia roots. It is shown according to experimental results that the traditional Chinese medicine composition combined with the chemotherapeutic drug cisplatin can obviously inhibit the growth and metastasis of liver cancer orthotopic transplanted tumors, can reduce the toxic and side effects of the chemotherapeutic drug, prevent the damage of the cisplatin to immune organs and improve the inhibition effect of the cisplatin on bone marrow. The traditional Chinese medicine composition combined with the chemotherapeutic drug cisplatin induces apoptosis of the human hepatoma cells HepG2, which indicates that the combined useof the traditional Chinese medicine composition and the chemotherapeutic drug cisplatin can produce effect-enhancing and toxicity-reducing action, and the traditional Chinese medicine can be used asan adjuvant radio therapy and chemotherapy medicine for clinically treating tumors.

Description

technical field [0001] The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition for adjuvant treatment of liver cancer, which belongs to the technical field of medicine. Background technique [0002] Hepatocellular carcinoma is the fifth most common cancer worldwide and the third leading cause of cancer mortality. One of the toughest and deadliest forms of cancer, most cases are diagnosed at advanced stages, with underlying liver dysfunction and often resistant to conventional chemotherapy and radiation treatments. It is still necessary to find an effective strategy to prolong the lifespan of HCC patients. The chemotherapeutic drug cisplatin, once known as the "penicillin in the field of antineoplastic drugs", exerts cytotoxicity by binding to genomic DNA in the nucleus, and has been used to treat various cancers. However, the main reason limiting the use of cisplatin is drug resistance due to altered ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9066A61P35/00A61P35/04A61P37/02A61P19/00A61K33/243
Inventor 唐德才姚映芷顾俊菲尹刚张硕
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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