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Application of Vietnamese ginsenoside R4 in preparation of medicament for treating neurodegenerative diseases

A neurodegenerative, Vietnamese ginseng technology applied in the field of medicine to achieve the effect of reducing cell damage

Active Publication Date: 2019-05-17
HUAIYIN TEACHERS COLLEGE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vietnamese ginsenoside R4 is one of the active ingredients of saponins, but whether Vietnamese ginsenoside R4 can have a neuroprotective effect on neurons damaged by PD disease has not been reported at home and abroad.

Method used

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  • Application of Vietnamese ginsenoside R4 in preparation of medicament for treating neurodegenerative diseases
  • Application of Vietnamese ginsenoside R4 in preparation of medicament for treating neurodegenerative diseases
  • Application of Vietnamese ginsenoside R4 in preparation of medicament for treating neurodegenerative diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 MTT method detects the toxic effect of R4 on normal PC12 cells

[0044] Experimental materials: Rat pheochromocytoma PC12 was purchased from the American Type Culture Collection (ATCC)

[0045] Experimental procedure: Digest PC12 cells in the logarithmic phase with 0.25% trypsin-EDTA, and then add high-quality protein supplemented with 10% fetal bovine serum, 5% horse serum, 100 μg / mL streptomycin, and 100 units / mL penicillin. Sugar DMEM medium to pipette the cells down to single cells; 5 Cells / mL were inoculated on 96-plates, and drug treatment was performed according to different experimental groups. Among them, the blank group is DMEM complete medium without PC12 cells; the control group is the cell culture medium without R4 and 6-OHDA; liquid.

[0046] The culture medium of each group was in CO 2 Cultivate in the incubator for 24 hours, remove the cell culture medium with a vacuum pressure pump, add 100 μL of MTT stock solution with a final concentrati...

Embodiment 2

[0049] Example 2 MTT method to detect the influence of R4 on the viability of PC12 cells induced by 6-OHDA

[0050] Experimental procedure: Cells in the logarithmic phase were divided into 3×10 5 Cells / mL were seeded on 96-plates and cultured for 24 hours. Set up control group, blank group, 250μM 6-OHDA treatment group, 250μM 6-OHDA+25μM R4 treatment group, 250μM 6-OHDA+50μM R4 treatment group, 6-OHDA+100μM R4 treatment group. In the experimental group, different concentrations of R4 were added for pretreatment for 30 minutes, and then 250 μM 6-OHDA was added for 24 hours. The cell survival rate of each group was measured by MTT method, and the specific steps were as described in Example 1.

[0051] Experimental results such as figure 2 shown by figure 2 It can be seen that the cell survival rate of the 6-OHDA treatment group was 58.76%. Compared with the 6-OHDA treatment group, the cell survival rate of the R4 pretreatment group with different concentrations increased s...

Embodiment 3

[0052] Example 3 Effect of R4 on PC12 Cell Morphology Induced by 6-OHDA

[0053] Experimental steps: Scanning electron microscopy (SEM) was used to observe the morphology of PC12 cells induced by 6-OHDA, cell grouping settings: normal control group, 250 μM 6-OHDA treatment group, 250 μM 6-OHDA+25 μM R4 treatment group, 250 μM 6-OHDA+ 50 μM R4 treatment group, 250 μM 6-OHDA+100 μM R4 treatment group. After cell drug treatment, fix the cells with 2.5% glutaraldehyde fixative, incubate in the incubator for 1 h, wash the cells with PBS solution for 3 times, each time for 5 min, and finally use 30%, 50%, 70%, 90% , 100% ethanol gradient elution to wash the cells twice, 5 min each time. Take the treated cells and observe their cell morphology under SEM.

[0054] See the experimental results image 3 shown by image 3 (a) (Normal control group) The results show that normal PC12 cells have protrusions, and the cells are closely connected to each other to form a network structure; ...

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Abstract

The invention provides application of Vietnamese ginsenoside R4 in preparation of a medicament for treating neurodegenerative diseases, in particular to the application in preparation of a medicamentfor treating Parkinson's disease. According to the application, by designing an in-vitro Parkinson model, Vietnamese ginsenoside R4 is proposed for the first time and proves to be capable of reducingcell damage, and the resistance effect on Parkinson disease is achieved by inhibiting oxidative stress of cells and regulating cell apoptosis. Therefore, the application of Vietnamese ginsenoside R4 as an effective medicament for treating Parkinson disease provides a basis for developing and expanding the medicament application of Vietnamese ginsenoside R4.

Description

technical field [0001] The present invention relates to the field of medicines, in particular to the application of Vietnamese ginsenoside R4 in the preparation of drugs for treating neurodegenerative diseases, especially the application in the preparation of drugs for Parkinson's disease. Background technique [0002] Neurodegenerative disease is a disease of central nervous system dysfunction caused by the loss of neurons or myelin sheath, which is mainly in the middle and old age. Alzheimer's disease, Parkinson's disease, and Huntington's disease are relatively common neurodegenerative diseases. [0003] Parkinson's disease (PD) is a neurodegenerative disease that occurs in the elderly population and ranks second only to Alzheimer's disease. It is caused by the selective death or loss of dopaminergic neurons in the substantia nigra and intracellular Lewis body formation. The clinical features of PD disease are slowness of movement, posture and gait disturbance, resting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/58A61P25/00A61P25/16A61P7/00A61P39/06A61P43/00
Inventor 胡卫成罗艳艳李甫沈婷袁巧云
Owner HUAIYIN TEACHERS COLLEGE
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