Optical probe based on gold nanoparticle efficient assembly structure, and preparation method and application of optical probe

A gold nanoparticle, optical probe technology, applied in measurement devices, scientific instruments, instruments, etc., can solve the problems of long detection time, poor probe fluidity, etc., to improve detection sensitivity, good stability and resistance. Effects of Interference Ability

Active Publication Date: 2019-05-14
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Liu Guodong et al reported the detection of proteins by loading gold nanoparticles on silicon nanorods as probes for immunochromatographic test strips. Although the sensitivity has been improved, the loading of gold nanoparticles is only limited to the surface of silicon nanorods. , and the fluidity of the probe becomes worse, and the detection time becomes longer, so there is still room for improvement

Method used

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  • Optical probe based on gold nanoparticle efficient assembly structure, and preparation method and application of optical probe
  • Optical probe based on gold nanoparticle efficient assembly structure, and preparation method and application of optical probe
  • Optical probe based on gold nanoparticle efficient assembly structure, and preparation method and application of optical probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. Synthesis of gold nanoparticles:

[0049] Take 824 μL tetrachloroauric acid stock solution (1g tetrachloroauric acid dissolved in 4mL ethanol) in a 50mL three-necked flask, drain the ethanol, keep the bottle full of argon after degassing, then add 25mL toluene, 2.5 mL oleylamine, stirred rapidly at 100°C for 6h. After the reaction, an equal volume of ethanol was added, and after shaking, a precipitate was formed, then centrifuged, and the obtained precipitate was dissolved in 10 mL of chloroform, and set aside.

[0050] 2. Synthesis of thiol dendritic mesoporous silica sphere template:

[0051] First, 68 mg of TEA was added to 25 mL of water and stirred at 80° C. for 30 minutes, then 380 mg of CTAB and 168 mg of sodium salicylate were added and stirred for 1 hour. 4 mL of TEOS was injected into the above solution, and stirred slowly at 80° C. for 4 hours. The product was centrifuged and washed three times with ethanol, and finally dispersed in a mixed solution of ...

Embodiment 2

[0059] 1. Synthesis of gold nanoparticles:

[0060] Take 824 μL tetrachloroauric acid stock solution (1g tetrachloroauric acid dissolved in 4mL ethanol) in a 50mL three-necked flask, drain the ethanol, keep the bottle full of argon after degassing, then add 37.5mL toluene, 3.75mL oleylamine was stirred rapidly at 110°C for 6h. After the reaction, an equal volume of ethanol was added, and after shaking, a precipitate was formed, then centrifuged, and the obtained precipitate was dissolved in 10 mL of chloroform, and set aside.

[0061] 2. Synthesis of thiol dendritic mesoporous silica sphere template:

[0062] First, 68 mg of TEA was added to 25 mL of water and stirred at 80° C. for 30 minutes, then 380 mg of CTAB and 168 mg of sodium salicylate were added and stirred for 1 hour. 4 mL of TEOS was injected into the above solution and stirred slowly at 80 °C for 4 hours, the product was centrifuged and washed 3 times with ethanol. The above experiments were repeated, and the f...

Embodiment 3

[0070] 1. Synthesis of gold nanoparticles:

[0071] Take 824 μL tetrachloroauric acid stock solution (1g tetrachloroauric acid dissolved in 4mL ethanol) in a 50mL three-necked flask, drain the ethanol, keep the bottle full of argon after degassing, then add 25mL toluene, 2.5 mL of oleylamine was stirred rapidly at 110°C for 6h. After the reaction, an equal volume of ethanol was added, and after shaking, a precipitate was formed, then centrifuged, and the obtained precipitate was dissolved in 10 mL of chloroform, and set aside.

[0072] 2. Synthesis of thiol dendritic mesoporous silica sphere template:

[0073] First, 68 mg of TEA was added to 25 mL of water and stirred at 80° C. for 30 minutes, then 380 mg of CTAB and 218 mg of sodium salicylate were added and stirred for 1 hour. 4 mL of TEOS was injected into the above solution, and stirred slowly at 80° C. for 3 hours. The product was centrifuged and washed three times with ethanol, and finally dispersed in a mixed soluti...

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Abstract

The invention provides an optical probe based on a gold nanoparticle efficient assembly structure, and a preparation method and an application of the optical probe. Firstly, a high-load treelike mesoporous silicon ball / gold nanoparticle assembly is prepared by utilizing a sulfydryl-metal affinity effect in an organic phase; n-octyltrimethoxysilane / methanol / ammonia water serves as a hydrolysis system; silylation modification of a hydrophobic assembly is realized; the ultra-high load capacity of a treelike mesoporous silicon ball carrier to gold nanoparticles in a modification process is ensured; microspheres with stable properties and excellent performances are prepared through hydrolytic condensation of organosilane and growth process of Stober; and the microspheres are applied to an immunochromatographic test strip after being further carboxylated and functionalized by using an anti-methamphetamine, so that methamphetamine can be detected rapidly, specifically, accurately and sensitively within 15 minutes.

Description

(1) Technical field [0001] The invention relates to an optical probe based on an efficient assembly structure of gold nanoparticles and a preparation method thereof, as well as an application in rapid drug detection. (2) Background technology [0002] Methamphetamine (MA), commonly known as methamphetamine, is a highly toxic stimulant drug. Long-term overdose can lead to confusion, hallucinations, weight loss, organ failure, and blood infection with bacteria and viruses. As the problem of methamphetamine abuse has become particularly prominent in recent years, it is very important to quickly and accurately detect methamphetamine in order to combat drug abuse. Now common detection methods include high performance liquid chromatography, gas-mass spectrometry, thin-layer chromatography, capillary electrophoresis, solid-phase extraction and so on. These methods have the advantages of high detection sensitivity and strong specificity, but these methods also have the disadvantage...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/543
Inventor 黄亮金洁宁胡军汪晶
Owner ZHEJIANG UNIV OF TECH
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