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Application of cyclosporine A in drugs in combination with hyperthermia for treatment of cervical cancer

A technology of combined application and cervical cancer, applied in the field of medicine, can solve the problems of unreported effects of cervical cancer cells and tumors, and achieve the effects of inhibiting proliferation and inducing apoptosis, inducing apoptosis, and being suitable for large-scale production.

Inactive Publication Date: 2019-05-14
HEBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In summary, the pharmacological effects of CysA may far exceed people's current understanding, and its effect on cervical cancer cells and tumors has not been reported

Method used

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  • Application of cyclosporine A in drugs in combination with hyperthermia for treatment of cervical cancer
  • Application of cyclosporine A in drugs in combination with hyperthermia for treatment of cervical cancer
  • Application of cyclosporine A in drugs in combination with hyperthermia for treatment of cervical cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The effect of combined treatment of CysA and hyperthermia on the survival rate of human cervical cancer HeLa cells detected by trypan blue staining

[0029] Experimental principle

[0030] Trypan blue dye does not permeate normal, intact cell membranes of live cells, so live cells do not stain. However, the increased permeability of the cell membrane of dead cells allows the dye to enter the cell through the cell membrane, making the dead cells stained blue. Trypan blue staining is the most commonly used experimental method to detect cell viability.

[0031] Experimental Instruments and Materials

[0032] Cell culture incubator (Thermo Fisher), trypan blue dye (Thermo Fisher), microscope (BIO-TEC), fetal bovine serum (Biological Industries), DMEM (high glucose) medium (GIBCO), cyclosporine A (MCE) , 6cm cell culture dish (NEST), HeLa cell line (Shanghai Institute of Cells, Chinese Academy of Sciences), electric thermostatic water bath (DK-8D type).

[0033] Experime...

Embodiment 2

[0039] MTT method to detect the effect of CysA combined with hyperthermia on the viability of human cervical cancer HeLa cells

[0040] Experimental principle

[0041] The detection principle is that succinate dehydrogenase in the mitochondria of living cells can make exogenous 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) is reduced to water-insoluble blue-purple crystalline formazan (Formazan) and deposited in cells, while dead cells have no such function. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and its light absorption value is measured at a wavelength of 490nm with a microplate reader. The amount of MTT crystal formation is proportional to the number of cells. According to the measured absorbance value (OD value), the number of living cells is judged. The larger the OD value, the stronger the cell activity. MTT method is a fast, simple and semi-automatic method for quantitative analysis of cell proliferation and drug cytotoxicit...

Embodiment 3

[0050] Western Blot detection of the effect of combined treatment of CysA and hyperthermia on the expression of apoptosis regulatory proteins in human cervical cancer HeLa cells

[0051] Experimental principle

[0052] Western Blot uses polyacrylamide gel electrophoresis, the detected object is protein, the "probe" is an antibody, and the "color development" uses a labeled secondary antibody. SDS-PAGE can separate protein samples and transfer them to a solid support such as polyvinylidene fluoride membrane (PVDF). Solid phase supports can adsorb proteins and keep the types of polypeptides separated by electrophoresis and their biological activities unchanged. The transferred PVDF membrane is called a blot, which is treated with a protein solution (such as 5% skim milk powder solution) to block the hydrophobic binding sites on the PVDF membrane. Treat the PVDF membrane with the antibody of the target protein (primary antibody)—only the protein to be studied can specifically b...

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Abstract

The invention discloses application of cyclosporin A in drugs in combination with hyperthermia for treatment of a cervical cancer. Combination treatment of the cyclosporine A and thermoexcitation on HeLa cells can significantly induce apoptosis of cancer cells and reduce the survival rate of the cancer cells, thereby achieving the effect of treating tumors, and important ideas are provided for clinical treatment of the tumors.

Description

technical field [0001] The invention relates to the application of cyclosporine A in combination with hyperthermia to treat cervical cancer, and belongs to the field of medicine. Background technique [0002] Cervical cancer is one of the most common female reproductive tract malignancies. Although domestic vaccines for the prevention of cervical cancer are already on the market, the incidence of cervical cancer in China is still on the rise and remains high due to reasons such as high price, long cycle, and unknown long-term effect. Every year, the number of new cases and deaths of cervical cancer has exceeded 1 / 4 of the world's total. Its morbidity and mortality rank first among gynecological malignancies in China, and the age of onset of cervical cancer tends to be younger. [0003] At present, the treatment of cervical cancer patients is still based on comprehensive treatment such as surgery, radiotherapy, and neoadjuvant chemotherapy. The patient's survival and qualit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/13A61K41/00A61P35/00
Inventor 谭克樊玉梅邵静宇
Owner HEBEI NORMAL UNIV
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