Method for keeping cordyceps sinensis fresh through modified atmosphere packaging containing argon
A technology of cordyceps sinensis and modified atmosphere packaging, which is applied in the field of preservation of cordyceps sinensis to achieve the effect of slowing down the decline of soluble protein content, maintaining better color and fragrance, and inhibiting the decline of weight loss rate
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[0065] In the present invention, the chitosan-glucose complex plays the role of forming a protective film with functions such as antibacterial, anti-oxidation, and respiration inhibition in the coating solution. In the present invention, the preparation method of described chitosan glucose complex preferably comprises the following steps:
[0066] A1, dissolving chitosan and glucose in glacial acetic acid, is mixed with the glacial acetic acid solution containing volume concentration 0.5~2% chitosan and volume concentration 0.5~2% glucose;
[0067] A2, will contain the glacial acetic acid solution of volume concentration 0.5~2% chitosan and volume concentration 0.5~2% glucose at 103.4KPa, 121.3 ℃ (1.05kg / cm 2 ) under the condition of reacting for 15 to 20 minutes, and freeze-drying to obtain the chitosan-glucose complex.
[0068] In the present invention, in the step A1, the preferably formulated glacial acetic acid solution contains chitosan at a volume concentration of 1-1....
Embodiment 1
[0078] Preservative solution was prepared: 1 mmol / L sorbitol, 1 mmol / L iso-Vc, 6 μmol / L phytic acid, and the concentration of L-cysteine was 0.4 mg / kg.
[0079] Preparation of the ethanol extract of the Cordyceps sinensis fermentation liquid: inoculate the Cordyceps sinensis strain into a liquid fermentation medium (including potatoes, soybeans, sucrose, glucose, peptone, yeast extract, mineral salts and vitamins) after activation, and cultivate at 26°C for 7 days. Take the supernatant by centrifugation to obtain the Cordyceps fermented liquid; add ethanol to the Cordyceps fermented liquid until the volume percent concentration of ethanol is 75%, stop, collect the supernatant by centrifugation, distill under reduced pressure to remove the ethanol in the supernatant, freeze-dry , to obtain the ethanol extract of Cordyceps sinensis fermentation broth.
[0080] The preparation of chitosan-glucose complex: configuration mass percent concentration 1% chitosan, the glacial acetic ...
Embodiment 2
[0086] Preservative solution was prepared: 3mmol / L sorbitol, 3mmol / LVc, 9μmol / L phytic acid, and the concentration of L-cysteine was 0.8mg / kg.
[0087] Preparation of the ethanol extract of the Cordyceps sinensis fermentation liquid: inoculate the Cordyceps sinensis strain into a liquid fermentation medium (including potatoes, soybeans, sucrose, glucose, peptone, yeast extract, mineral salts and vitamins) after activation, and cultivate at 25°C for 7 days. Take the supernatant by centrifugation to obtain the Cordyceps fermented liquid; add ethanol to the Cordyceps fermented liquid until the volume percentage concentration of ethanol is 70%, stop, collect the supernatant by centrifugation, distill under reduced pressure to remove the ethanol in the supernatant, freeze-dry , to obtain the ethanol extract of Cordyceps sinensis fermentation broth.
[0088] The preparation of chitosan-glucose complex: configuration mass percent concentration 1% chitosan, the glacial acetic acid m...
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