Pine flower active peptide, composition containing pine flower active peptide, fungus slices and preparation process thereof
A technology of active peptides and compositions, applied in the field of compositions containing the active peptides, can solve problems such as physical disadvantage, easy rebound, intestinal dysfunction, etc., to prolong the enzymatic hydrolysis time, be easy to operate, and reduce the enzymatic hydrolysis. effect of speed
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Embodiment 1~6
[0038] A kind of pine flower active peptide is obtained by hydrolyzing pine flower protein isolate successively with pepsin and alkaline protease, and the specific steps are as follows:
[0039] Step 1: Enzymatic hydrolysis:
[0040] Step A: First, add water to the pine flower protein isolate, and prepare a pine flower protein solution with a mass concentration of 2g / 100mL as a substrate; use hydrochloric acid to adjust the pH of the substrate solution to 4.2, and heat it to 37 degrees Celsius. Add 1100U / g of pepsin to the substrate solution, enzymatically hydrolyze it for 15min under the condition of 37 degrees Celsius and pH4.2, then use sodium hydroxide to adjust the pH of the substrate solution to 8.0, and heat up to 54 degrees Celsius. 2606U / g of alkaline protease was added thereto, enzymatically hydrolyzed for 30min at 54°C and pH8.0, and then inactivated in a boiling water bath for 15min; enzymatic hydrolyzate A was obtained;
[0041] Step B: the enzymolysis time of pe...
Embodiment 2
[0048] Embodiment 2 is: collect the pine flower active peptide of 1~3kDa;
Embodiment 3
[0049] Embodiment 3 is: collect the Songhua active peptide of 3~5kDa;
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